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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia VALENSI Jurnal Peternakan Integratif Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Jurnal Ilmiah Ilmu Terapan Universitas Jambi Jurnal Peternakan Integratif al Kimiya : Jurnal Ilmu Kimia dan Terapan Journal of Engineering and Technological Sciences

YAYA RUKAYADI

Departemen Sains Makanan, Fakultas Sains Dan Teknologi Makanan, Universiti Putra Malaysia, Serdang- Salangor 43400, Malaysia

Author-ID : 365316

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Seleksi, Karakterisasi Morfologi, dan Identifikasi Aktinobakteri Penghasil Mananase Asal Hutan Tanah Jambi untuk Produksi Mananoligosakarida Rahayu Wulan; Rika Indri Astuti; Yaya Rukayadi; Sri Estuningsih; Anja Meryandini
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.279

As the world's largest producer of crude palm oil, Indonesia also produces high palm kernel cake (PKC) by-products. PKC has a high mannan content, so it can be used to produce prebiotic mannan-oligosaccharides (MOS). Enzymatic MOS production can be carried out using actinobacterial mannanase's microbiological approach. The HJ45B-1 isolate was the best isolate, with a peak enzyme production of 0,338 U/mL on the 10th day of incubation. The mannanase enzyme was stable in storage at 27°C. MOS production using 1% PKC substrate produced MOS with the best degree of polymerization (2-4) with incubation for 1-3 hours. Morphological characteristics and molecular identification based on the 16S rRNA gene indicated that the HJ45B-1 isolate was Streptomyces spp. Keywords: actinobacteria, mannanase, mannan-oligosaccharides, palm kernel cake, Streptomyces

The Effects of Xanthorrhizol on the Morphology of Candida Cells Examined by Scanning Electron Microscopy YAYA RUKAYADI; JAE-KWAN HWANG
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

The effects of xanthorrhizol, a natural anticandidal agent isolated from the rhizome of temulawak or java turmeric (Curcuma xanthorrhiza Roxb.) on the morphology of four human pathogenic Candida species, i.e., C. albicans, C. glabrata, C. guilliermondii, and C. parapsilosis was examined by scanning electron microscopy (SEM). The SEM analysis showed that, unlike control cells representing normal oval to spherical with smooth surface, treatment of Candida strains with xanthorrhizol at 1 x MICs (minimum inhibitory concentration) significantly affected the external morphology, exhibiting deformation, and protrusions on the cell surface. The potent anticandidal activity of xanthorrhizol may support the use of medicinal plants for the treatment of candidal infections.

Time-Kill Assay of 4-Hydroxypanduratin A Isolated from Kaempferia Pandurata Against Foodborne Pathogens Soerya Dewi Marliyana; Didin Mujahidin; Yana M Syah; Yaya Rukayadi
Molekul Vol 12, No 2 (2017)
Publisher : Universitas Jenderal Soedirman

Time–kill assay was performed for 4-hydroxypanduratin A that was isolated from Kaempferia pandurata rizhome against four important foodborne pathogens, namely Bacillus cereus ATCC 21772, Bacillu subtilis ATCC 6633, Staphylococcus aureus ATCC 29737, and Proteus mirabilis ATCC 21100. The methods have been investigated in term of Minimum Inhibitory Concentration (MIC) and killing time curve using methods of Clinical and Laboratory Standards Institute (CLSI) guidelines. The results showed that 4-hydroxypanduratin A rapid acting in killing bacteria as follow: B. cereus : 1×MIC for 4 h, P. mirabilis: 4×MIC for 0.5 h, meanwhile B. subtilis and S. aureus were 1×MIC for 2 h. In conclusion, 4-hydroxypanduratin A showed strong antimicrobial activity against four important foodborne pathogens.

A New Flavonoid from Malaysian Dipterocarpus cornutus Wan Zuraida Wan Mohd Zain; Liliwirianis Nawi; Norizan Ahmat; Che Puteh Osman; Yaya Rukayadi
Indonesian Journal of Chemistry Vol 21, No 5 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.61800

Dipterocarpus cornutus Dyer is commonly known as ‘keruing’. It belongs to the family of Dipterocarpaceae, an important timber family in South East Asia. D. cornutus is listed as critically endangered on IUCN Red List. Since no comprehensive study has been documented on the chemical constituents of D. cornutus, there is an urgent need to study this plant comprehensively. Phytochemical study of the stem bark of D. cornutus afforded a new flavonoid (1) and nine known compounds, which consist of flavonoids (2, 3), oligostilbenoids (4, 5, 7, 8, 9, 10), and coumarin (6). The finding of the study contributes to the chemotaxonomic differentiation in the plants of the tribe Dipterocarpae.

THE ROLE OF OMICS RESEARCH IN GERMPLASM CONSERVATION Yaya Rukayadi
Prosiding Seminar Nasional Biotik Vol 9, No 2 (2021): PROSIDING SEMINAR NASIONAL BIOTIK IX 2021
Publisher : Prosiding Seminar Nasional Biotik

The word omics refers to a field of study in biological sciences that ends with -omics, such as genomics, transcriptomics, proteomics, or metabolomics. The ending -ome is used to address the objects of study of such fields, such as the genome, proteome, transcriptome, or metabolome, respectively. In relation to the conservation of germplasm, genomics-based plant germplasm research has been carried out and has been proven to be able to conserve germplasm. Recently, to conserve germplasm using only genomics-based plant germplasm research, it is felt to be incomplete, because not all genes can be expressed under certain conditions. For this reason, other omics such as proteomics and metabolomics play an important role in the conservation of germplasm. In this paper, the role of other omics research, especially metabolomics is described.

Lignans from Phyllanthus niruri L. and Their Antifusarium Properties Neneng Windayani; Yaya Rukayadi; Yana Maolana Syah; Tri Cahyanto
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 8, No. 2, November 2022
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v8i2.25057

In this study, two lignan compounds were isolated from acetone extract of Phyllanthus niruri L. nirtetralin B (1) and phyllanthin (2) using several chromatographic methods followed by molecular structure elucidation mainly based on 1D and 2D of 1H and 13C NMR spectrum. The isolated compounds were tested for their antimicrobial properties against the plant pathogenic fungus, Fusarium oxysporum, using the agar plate well diffusion method. The microdilution method determined the minimum inhibitory concentration (MIC) and the minimum fungicide concentration (MFC). In addition, the microconidia germination inhibition test was carried out using the agar diffusion method. As a result, compound 1 had MIC and MFC values of 4 and 16 μg/mL, respectively. While compound 2 showed the same MIC and MFC values of 16 μg/mL. Further testing on the inhibition of germination of F. oxysporum microconidia showed that compound 2 inhibited microconidia germination 100% at a concentration of 2 × MIC. In comparison, compound 1 at the same concentration was only able to inhibit germination by 29%. This study revealed that compound 2 is a potential new fungicide derived from local medicinal plants. However, further research is needed to identify the interaction mechanism between the test compound and the fungal pathogen F. oxysporum to develop new antifungal agents.

Increase in Polyphenolic Substances from Fermented Robusta Coffee Pulp (Coffea canephora L.) by Using Indigenous Actinomycetes Eka Putri; Yaya Rukayadi; Titi Candra Sunarti; Anja Meryandini
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.457-465

The fermentation of the coffee fruit processing into coffee beans leaves the fermented coffee pulp which still contains polyphenol secondary metabolites. This study aimed to analyze the total flavonoid content (TFC) and total phenolic content (TPC) of fermented robusta coffee pulp (Coffea canephora L.) by using indigenous Actinomycetes. The ability of fermented extracts to inhibit free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) also were done. In this study, coffee cherries were fermented in the solid-state fermentation cultivation (SSF) using isolates HJ4.5b, P2b(b).3 and P2b(b).18 which in previous studies were reported to have cellulolytic and xylanolytic activities. Determination of molecular identification base on 16S rRNA gen showed the isolates HJ4.5b and P2b(b).3 have similarities to the genus Streptomycetes, while P2b(b).18 has a homologous base arrangement with a rare actinomycetes genus Micromonospora. Fermentation using P2b(b).3 on the 9th day of fermentation indicated the highest percentage increase in TFC (295.54%) with IC50 18.41 µl/ml and having an antioxidant activity index (AAI) value of 2.14 which was included in the very strong antioxidant activity category.

Antibacterial and Anticancer Activities of Acetone Extract Caesalpinia sappan L. Tita Juwitaningsih; Nurhadi Syahputra; Eddiyanto Eddiyanto; Neneng Windayani; Yaya Rukayadi
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 9, No 2 (2022): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v9i2.20966

Sappan wood (Caesalpinia sappan L.) from the Caesalpiniaceae family is a plant that has been widely used as a health drink such as herbal medicine, in Sundanese called jamu or wedang secang. The drink containing C.sappan extract is traditionally believed to have the ability to reduce symptoms of colds, coughs, canker sores, and rheumatism, overcome fatigue and improve blood circulation. In this research, wood extract of C. sappan L in acetone solvent tested as antibacterial and anticancer. The antibacterial test was performed on the bacteria Streptococcus mutans ATCC 35668 and Enterococcus. faecalis ATCC 49619 with paper disc diffusion method. The microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The anticancer test is carried out in vitro on A549 lung cancer cells using the MTT method assay. The results showed that the wood extract of C. sappan L in acetone has weak antibacterial activity against S. mutans ATCC 35668 and E. faecalis ATCC 49619. The MIC is 1250 ug/mL, respectively. Aceton wood extract of C. sappan L. showed moderate anticancer activity with IC50 value 90,01µg/mL. Thus, the extract of C. sappan L. in acetone has the potential as a source of anticancer compounds.

Phytochemical Screening and Evaluation of Antibacterial, Anticandidal, and Sporicidal Properties of Euphorbia tirucalli Extract in Terengganu, Malaysia Abd Wahab, Noor Zarina; Malza, Nur Maizatul Najwa Malza; Rukayadi, Yaya
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.903-915

Euphorbia tirucalli, commonly known as the pencil cactus or milk bush plant, is used as an alternative medicine. The current study evaluated the phytochemical contents, antibacterial, anticandidal, and antisporicidal potential of the E. tirucalli methanolic extract. The antibacterial and anticandidal activity of E. tirucalli methanolic extract was determined by performing a disc diffusion assay, MIC, MBC, and MCC. The sporicidal activity was tested at different concentrations of extract and exposure times. Phytochemical analyses revealed the presence of alkaloids, saponins, cardiac glycosides, terpenoids, and tannins in E. tirucalli methanolic extract. Results demonstrated inhibition zones of the extract against Gram-positive bacteria were in range of 22.00-7.00 mm. Meanwhile, inhibition zones of the extract against Gram-negative bacteria were in range of 13.00-7.00 mm. All bacteria were inhibited with MIC values at 1.56-25.0 mg/ml and can be completely killed with MBC values of 20-200 mg/ml. Inhibition zones of E. tirucalli methanolic extract against Candida spp. were in the range of 20.00-8.00 mm. All Candida spp. were inhibited with MIC values at 1.56-100.00 mg/ml and MBC values of 100-300 mg/ml. All concentrations of the extract inhibited all Bacillus spp. spores at different exposure times. In conclusion, the methanolic extract of E. tirucalli exhibits antibacterial, anticandidal, and sporicidal activities. The findings indicated that the methanolic extract of E. tirucalli has good potential for prospective nature-based antimicrobial agents.

PUMAKKAL FORMULA FOR MAKING SHRIMP POND WASTE FERTILISER Agus Sutanto; Kartika Sari; Handoko Santoso; Hening Widowati; Hasminar Rachman Fidiastuti; Yaya Rukayadi
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 8 No. 1 (2024): Volume 8, Nomor 1, June 2024
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v8i1.32094

One way to decompose pond waste sediment is by using the liquid nutrient culture media (NB) called Pumakkal as a starter formula. However, bioremediation in shrimp ponds has received less attention, despite being massively promoted by the government. East Lampung has produced 11.6 million m3 of liquid and 4.077 m3 of sediment waste in shrimp ponds. They are rich in organic matter, but possibly poison the pond if uncurbed. This study investigated in what manner Pumakkal decomposed shrimp pond waste under three parameters: macronutrient and micronutrient contents, and pH. The experiment was conducted using a completely randomised design (CRD) with 15 factorial arrangements. The sample was 65 kg of shrimp pond waste and 65 litres of liquid waste, which were analysed with five treatments: three of liquid waste media (LW), sediment waste (SW), and mixed liquid and sediment waste (MLS). The results showed that the treatment of the CE 15-isolate in MLS significantly improved the fertiliser quality (p<0.05). Pumakkal CE worked the finest in MLS, producing the fertiliser with the best macronutrients: Nitrogen (N) 1,3%, Phosphorus (P)2,3%, and Potassium (K) 2,3%; C-organic 23%, C/N ratio 29; micronutrient: Fe:155 ppm, Cu: 51 ppm, Zn: 72 ppm, Mn; 51 ppm, B; 25 ppm, and Mo: 8 ppm, and pH 5-6. The mixture of liquid and pond sediment waste produces the best fertiliser suitable for plant fertiliser users. The study concludes that Pumakkal is effective in decomposing harmful waste sediment to support the bioremediation program.

Co-Authors Abd Wahab, Noor Zarina Agus Sutanto Anja Meryandini Anja Meryandini Ashari, Rozzamri Azman, Ezzat Mohamad Chan, Hao Yuan Che Puteh Osman Didin Mujahidin Eddiyanto Eddiyanto Eka Putri Gabrina Tonyes, Silvia Handoko Santoso Hasminar Rachman Fidiastuti Hening Widowati JAE-KWAN HWANG Kartika Sari Liliwirianis Nawi Lim, Sarina Abdul Halim Malza, Nur Maizatul Najwa Malza Muheri Indra Aja Nasution Neneng Windayani Norizan Ahmat Nurhadi Syahputra Rahayu Wulan Rika Indri Astuti Soerya Dewi Marliyana Sri Estuningsih Tita Juwitaningsih Titi Candra Sunarti Tri Cahyanto Wan Zuraida Wan Mohd Zain Windayani, Neneng Yan Ramona Yana M Syah Yana Maolana Syah Yenni Ciawi

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