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All Journal HAYATI Journal of Biosciences Archive of Community Health Buletin Udayana Mengabdi Bumi Lestari BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrointek Jurnal Teknik Pengairan: Journal of Water Resources Engineering Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) ISM (Intisari Sains Medis) : Jurnal Kedokteran Cassowary AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Acitya Wisesa: Journal of Multidisciplinary Research Jurnal Pengabdian Kepada Masyarakat Widya Mahadi Nata Palemahan: Journal of Environmental Engineering Innovations Metamorfosa: Journal of Biological Science Open Access DRIVERset
Yan Ramona
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Social Sciences Veterinary

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GC-MS Analysis of Active and Applicable Compounds in Methanol Extract of Sweet Star Fruit (Averrhoa carambola L.) Leaves Ni Putu Adriani Astiti; Yan Ramona
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.12

Abstract

The leaves of star fruit plants (Averrhoa carambola) have been traditionally used to cure many diseases, such as hypertension and fever. Besides, the leaves of this plant have also widely been used as the main raw material in lawar (a Balinese traditional food) making. In this research, the methanol extract of this plant leaves was analyzed and identified for active compound contents. The main objective of this research was to investigate types of possible active and applicable compounds contained in such leaves, previously extracted/macerated in methanol solution. Analysis was conducted by applying GC-MS instrumentation using methanol solution. The results showed that 10 possible active and applicable compounds (Butane, 1,1-diethoxy-3 methyl-(CAS)1.1-DII, Dodecanoic acid, methyk ester(CAS) methyl, Dodecanoic acid, methyl ester (CAS) Ethyl Laun, Pentadecanoic acid ethyl ester, Hexadecanoic acid methyl ester (CAS) Methyl pa, OCTADECA 9.12 DIENOIC ACID METHYL, 9-Octadecenoic acid methyl ester (E)-(CAS), Octadecanoic acid methyl ester, and (E) 9-Octadecanoic acid ethyl ester) were identified in methanol extract of such leaves. Among those compounds, Butane, 1,1-diethoxy-3-methyl and Hexadecanoic acid, methyl ester was two most abundance constituents with percentage of peak areas of 35.67% and 26.93%, respectively.
(RESISTANCE OF Lactobacillus spp. FBB TO SIMULATION OF UPPER PART INTESTINAL TRACT CONDITION FOR PROBIOTIC DEVELOPMENT) Ida Ayu Sri Sinca Maha Uni; Yan Ramona; I Nengah Sujaya
ARCHIVE OF COMMUNITY HEALTH Vol 2 No 2 (2013): Desember (2013)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

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Abstract

Lactobacillus spp. FBB (isolates 60, 72, dan 75), isolated from feces of healthy infants, were tested for resistance in a modified gastric juice in vitro, before being applied in in vivo tests. These isolates were exposed for 7 hours in the modified gastric juice (added with pepsin, pancreatin, and NaDC) with various pH of 2, 3, 4, or 8. Isolates of FBB that showed the most resistant properties in this test was identified by applying molecular method or by sequencing its 16S rDNA. In this test, the FBB isolates showed various degree of resistance to the modified gastric juice conditions. FBB72 was found to be more resistant than the two other isolates. This isolate appeared to have the ability to reach colon (viable when reach colon), although its density was very low (104 CFU/ml and 103 CFU/ml at pH 4 and 3, respectively). Based on its 16s rDNA nucleotide sequence, this FBB72 was found to be closely related to Lactobacillus rhamnosus GG ATTCC53103 with 99% similarity.
EFEK KONSUMSI BIO-YOGHURT DARI LACTOBACILLUS RHAMNOSUS SKG34 TERHADAP LIPID PROFILE PADA MANUSIA I Desak Putu Kartika Pratiwi; Komang Ayu Nocianitri; Wayan Redi Aryanta; Yan Ramona; I Dewa Made Sukrama; I Nengah Sujaya
ARCHIVE OF COMMUNITY HEALTH Vol 2 No 1 (2013): Juni (2013)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

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Abstract

This research was carried out to elucidate the eff ect of consumption bio-yoghurt produced byLactobacillus rhamnosus SKG34 on plasma lipid profi les in human. The study was performed usingpre and post analysis. Eleven healthy volunteers aged between 30-48 yeras old were participatedin this study. Each volunteers consumed 100g of bio-yoghurt containing L.rhamnosus SKG34 for 21 days. Blood samples were taken before the start of experiment and after 21 days of comsumption. Total cholesterol was measured using CHOP-POD method, while triglyceride determinedusing glycerol method, LDL and HD determined using homogenous method. Results of thestudy revealed that consumption of the bio-yoghurt lower values of plasma lipid concentrationsin hypercholesterolemia subjects. Total cholesterol, triglyceride and LDL was reduced within4.91%, 13.84%, and 8.24%, respectively, when compare to its concentrations before treatment.However, the plasma lipid concentrations in normal subjects were almost stable during thetreatment periods. Consumption of bio-yoghurt for 21 days had no eff ect on HDL-cholesterollevel. These results indicate that consumption of bio-yoghurt containing L.rhamnosus SKG34specifi cally lowered the concentrations of serum total cholesterol, LDL, and triglyceride onhypercholesterolemia subjects.
MODEL PEMBINAAN ‘IN-HOUSE TRAINING’ SEBAGAI UPAYA UNTUK MENINGKATKAN KEMAMPUAN GURU DALAM PENELITIAN DAN PENULISAN KARYA ILMIAH K. Dharmawan; Y. Ramona; N. N. Rupiasi
Buletin Udayana Mengabdi Vol 15 No 2 (2016): Jurnal Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Various attempts have been made by the government to improve the quality of teachers and quality ofeducation, such as allowances certification, training or the opportunity to continue to pursue education toMaster degree. However, these efforts have not able to improve the ability of research as mandated in theMinister Regulation PAN No. 16 Year 2009 Clause 11 that concerning the obligation of teachers in scientificpublications. To assist teachers in implementing the mandate, it is necessary to develop a model of coachingso that teachers can improve their competence and /or teacher professionalism in research. In this paper, it isdeveloped model guidance for teachers in improving the professionalism of teachers in research. The methodused in the development of this coaching model is a method of in-house training, the coaching done bysetting their respective schools, where teachers implement instructional programs. This paper is the result ofthe program community service activities are funded by DIKTI in the scheme of science and technology forsociety that was summarized in the form of coaching model through in-house training which consists of threestages approaches, namely scientific, competence, and publications. The results of this development showeda significant increase, approximately 30% on a teacher's ability to perform research of class action.
PENINGKATAN KUALITAS DAN NILAI FES NIRA AREN DI DESA TARO TEGALLALANG MELALUI DIVERSIFIKASI PRODUK OLAHAN D.A. Swastini; Y. Ramona; C.I.S. Arisanti
Buletin Udayana Mengabdi Vol 17 No 1 (2018): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.308 KB) | DOI: 10.24843/BUM.2018.v17.i01.p07

Abstract

Sucrose, electrolyte, vitamin, as well as probiotic bacterial contents of palm juice (Arrenga pinnata Merr) areuseful for the maintenance of the healthiness of human body. Due to its low glycemic index, palm sugar issafe to consume and has high potential for use as an alternative companion sugar for diabetes mellituspatients. In Taro village, almost 90% of the people there manage palm sugar plantations, but their ability toprocess and distribute their products is still limited. This program of Science and technology for the Societyaims to improve the quality and value of FEs palm juice by applying revitalization and diversificationmethods. Revitalization was conducted by socializing pre and postharvest preparation techniques andprocedures and the outcomes were measured by questionnaires. Diversification methods were conductedthrough training and advisory preparation of isotonic and probiotic drinks, as well as production of sugarcrystal. The results of this socialization showed that there was an increase in average knowledge of farmersfrom 22.57% (before socialization) to 73.87% (after socialization). Product diversification did not onlyincrease the value of FEs palm juice, but also saved energy and time of processing. As the product wassupported by credible results of laboratory tests, this product is currently marketed in some touristdestinations, such as Tegallalang, Gianyar.
PENDAMPINGAN KEGIATAN EKSRAKURIKULER KIR DALAM UPAYA MENINGKATKAN KUALITAS SEKOLAH SMA DWIJENDRA DENPASAR Komang Dharmawan; Y. Ramona; N. N. Rupiasih; I G. A. Widagda
Buletin Udayana Mengabdi Vol 19 No 3 (2020): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Youth Scientific Work (KIR) basically aims to trigger curiosity about natural phenomena related to science and technology. KIR can also increase the ability to think critically about natural phenomena and increase creativity that fosters creative ability and critical thinking. The purpose of this assistance is to introduce research methods for groups of teachers so students get better quality coaching. The mentoring method applied is In-House-Training, which is the implementation of mentoring at the relevant school. In this assistance 4 scientific works have been produced by high school students of Dwijendra Denpasar who are ready to be presented in KIR competitions both regionally and nationally.
MIKROENKAPSULASI PROBIOTIK LACTOBACILLUS RHAMNOSUS FBB81 DAN VIABILITASNYA SELAMA PENYIMPANAN Komang Ayu Nocianitri; I Nengah Sujaya; Yan Ramona
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Lactobacillus rhamnosus FBB81 is a probiotic candidate isolated from healthy infant feces that hasfunctional properties as an antioxidant. Probiotics are made in the form of a dry cell bymicroencapsulation technique to facilitate the application of probiotics in the development of functionalfood products. The purpose of this study was to determine the viability of Lb. rhamnosus FBB81encapsulated with maltodextrin and skim milk during storage. The microencapsulated product wasstored at two storage temperatures namely room temperature and cold temperature. The viability of Lb.rhamnosus FBB81 on the microencapsulated product was observed every 1 week for 8 weeks of storage.The results showed that the types of encapsulan and storage temperature affected the viability of Lb.rhamnosus FBB81 during storage. Skim milk and cold temperatures have a better ability than themaltodextrin and room temperature in maintaining the viability of probiotic Lb. rhamnosus FBB81during storage.
Antioxidant Activity of Lactobacillus spp as for Development of Antioxidantive Probiotic Komang Ayu Nocianitri; I Nengah Sujaya; Yan Ramona
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 1 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Probiotics are live microorganisms which when administered in adequate amount confer health benefit to the host. Our previous studies revealed that Lactobacillsus spp isolated from infant feces potential to be developed as probiotics. Since the functionality of probiotic is strain specific, it is worth to screen the antioxidant activity of those lactobacilli, which will be developed as antioxidative probiotic. The aim of this study was to screen antioxidant activities of Lactobacillus spp isolated from infant feces. A numbers of 20 Lactobacillus spp strains isolated from infant feces were used in this study. The antioxidant activities was determined in in vitro studies by measuring the lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity. The result showed that inhibitory activity of lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity exerted by of Lactobacillus spp was 10.12 - 83.02%, 16,50-46,73%, and 3.94 - 44.52%, respectively. The Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 showed the high antioxidant activity compared to the others lactobacilli. The antioxidant activity of Lactobacillus sp FBB 60 were 61,20%, 46,37%, 31,54%  and the activity of Lactobacillus sp FBB 81 were 57,01%, 29,31% 44,52 for the lipid peroxidation inhibitory activity, hydroxyl radical scavenging activity and ferrous chelating activity, respectively. This finding implied that the Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 are promising strain to be developed as antioxidative probiotic.
Antioxidant Activity of Lactobacillus spp as for Development of Antioxidantive Probiotic Komang Ayu Nocianitri; I Nengah Sujaya; Yan Ramona
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 1 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Probiotics are live microorganisms which when administered in adequate amount confer health benefit to the host. Our previous studies revealed that Lactobacillsus spp isolated from infant feces potential to be developed as probiotics. Since the functionality of probiotic is strain specific, it is worth to screen the antioxidant activity of those lactobacilli, which will be developed as antioxidative probiotic. The aim of this study was to screen antioxidant activities of Lactobacillus spp isolated from infant feces. A numbers of 20 Lactobacillus spp strains isolated from infant feces were used in this study. The antioxidant activities was determined in in vitro studies by measuring the lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity. The result showed that inhibitory activity of lipid peroxidation, hydroxyl radical scavenging activity, and ferrous chelating activity exerted by of Lactobacillus spp was 10.12 - 83.02%, 16,50-46,73%, and 3.94 - 44.52%, respectively. The Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 showed the high antioxidant activity compared to the others lactobacilli. The antioxidant activity of Lactobacillus sp FBB 60 were 61,20%, 46,37%, 31,54%  and the activity of Lactobacillus sp FBB 81 were 57,01%, 29,31% 44,52 for the lipid peroxidation inhibitory activity, hydroxyl radical scavenging activity and ferrous chelating activity, respectively. This finding implied that the Lactobacillus sp FBB 60 and Lactobacillus sp FBB 81 are promising strain to be developed as antioxidative probiotic.
Effectiveness of Sludge Microbial Consortia in the Bioremediation of Detergent-Containing Launderette Wastes Yan Ramona; Esti Arisetya Dewi; Yenni Ciawi; I Wayan Budiarsa Suyasa
Cassowary Vol 5 No 2 (2022): June
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v5.i2.162

Abstract

This project investigated the potential of microbial consortia obtained from several sources to reduce or eliminate detergent concentration in the wastes of launderette businesses found in the capital city of Bali. Before being used as active starters, the consortium of microbiota contained in the 3 waste sources was enriched in a medium containing (g/l); 1 glucose, 0.05 K2HPO4, 0.05 KH2PO4, 0.05 (NH4)2[Fe(SO4)2].6H2O, 0.01 MgSO4, 0.01 yeast extract, 1800 mL distilled water, and 200 mL of wastewater of launderette industries. An important parameter used to indicate microbial growth was the level of the volatile solid substrate (VSS) in the enrichment medium. The microbial culture that showed the highest VSS only was further investigated in the main experiments by observing its effectiveness in reducing detergent linear alkyl-benzenesulfonates (LAS) and the chemical oxygen demand (COD) of launderette wastes. In this project, microbial consortia developed from detergent-containing launderette wastes were the most active starter to decrease the COD and LAS detergent content following 7 days of exposure. This starter (microbial consortia developed from this launderette waste) showed the highest value of VSS when compared to those collected from other sources, and this was reached on day 4. In the main experiment, 85.5% and 91.9% reduction of LAS and the COD of the wastes, respectively, were observed following exposure of the launderette waste with this culture starter. This indicated that these microbial consortia have the possibility to be developed as a potential starter in larger scales of detergent bioremediation.
Co-Authors A. Line, Martin A.A. Ketut Darmadi Agestiawan, I. G. A. M. Agung Panji Dwipayana, I Dewa Alayna Himawan Anak Agung Gede Indraningrat Anak Agung Ketut Darmadi Anak Agung Ngurah Nara-Kusuma Ananda Sri Phartiswari Andriani, Luh Nyoman Ary Metta Anissa Maria Hidayati Ariwathi, Ni Luh Putu Ayu Amelia Bagas Susilo Batho, Yanuarius Felix Cok. Istri Dewiyani Cakrawati Cokorda Istri Sri Arisanti Dandy Bimantara Dewa Ayu Andriastini Dewa Ayu Swastini Dewantara, Putu Satrio Jhanardana Diah Kharismawati Djereng Diarsa, Made Okta Hapsari Putri Dwipayana, I Dewa Agung Panji Esti Arisetya Dewi Fainmarinat Selviani Inabuy Fainmarinat Selviani Inabuy Fernando Putra Flavia Fionessa Falenda Shanzet Fukuda Kenji Gabrina Tonyes, Silvia Gusti Ayu Dianti Violentina Gustu Widi Putra Hani, Ni Luh Helda Thorikul Affan I D.P. Kartika Pratiwi I Dewa Gede Cahyadi Kusuma I Dewa Made Sukrama I G. A. Widagda I Gusti Agung Gde Bayu Wirama I Gusti Ngurah Jemmy Anton Prasetya I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Oka Adi Parwata I Made Saka Wijaya I N.K. Widjaja I Nengah Sujaya I Putu Aditya Prayoga I Putu Oka Suartama I W BUDIARSA SUYASA I Wayan Suanda I Wayan Suardana I. M. A Sasmitha I.B. Oka Suyasa I.D.P.K. Pratiwi IBG Darmayasa Ida Ayu Ketut Ariningsih Ida Ayu Putu Suryanti Ida Ayu Sri Sinca Maha Uni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara Inabuy, Fainmarinat selviani Irma Damayanti K.R Suciptha Kadek Wegi Kurnilia KOMANG AYU NOCIANITRI Komang Dharmawan Kusuma, I Dewa Gede Cahyadi Lastini, Ni Putu Bintang Diah Meitini Proborini Wahyuni N. N. Rupiasi N.G.A Manik Ermayanti N.W.A. Utami Nadia Khairunnisa Nadya Treesna Wulansari Ni LUH ARPIWI Ni Luh Made Ema Juliasari Ni Luh Suriani Ni Made Susilawati Ni Made Teriyani Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Rupiasih Ni Putu Adriani Astiti Ni Putu Desy Aryantini Ni Putu Eka Leliqia Ni Putu Indiani Sahaning Karna Ni Putu Linda Laksmiani Ni Putu Suariani Ni Putu Widarini Ni Putu Widiantari Ni Wayan Eka Putri Gayatri Kastawa Ni Wayan Nursini NYOMAN SEMADI ANTARA Pande Gde Sasmita Julyantoro Pande Putu Ayu Sukmawati Putu Eka Sudaryatma Putu Rima Sintyadewi Retno Kawuri SANG KETUT SUDIRGA Satwika, Pande Gede Putra Sentot Joko Raharjo Septiari, I Gusti Ayu Agung Shetty, Kalidas Sri Wahyuni Sumartin N.W.E. Tadashu Urashima Teriyani, Ni Made Viviandari, I D. A. A Wayan Redi Aryanta Wulandari, Kristina YAYA RUKAYADI Yenni Ciawi Yoshitake Orikasa Yuji Oda Yuliastuti, Dwi Ariani Yulidia Iriani Yulidia Iriani Yuliya Ayu Lestari Yustiantara, Putu Sanna Zulfa, Rozifatul