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All Journal HAYATI Journal of Biosciences Archive of Community Health METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Farmasi Udayana Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari BIOTROPIA - The Southeast Asian Journal of Tropical Biology SIMBIOSIS Agrointek Jurnal Teknik Pengairan: Journal of Water Resources Engineering Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) ISM (Intisari Sains Medis) : Jurnal Kedokteran Advances in Tropical Biodiversity and Environmental Sciences Cassowary AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Acitya Wisesa: Journal of Multidisciplinary Research Jurnal Pengabdian Kepada Masyarakat Widya Mahadi Nata Palemahan: Journal of Environmental Engineering Innovations
Yan Ramona
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Social Sciences Veterinary

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GC-MS Analysis of Active and Applicable Compounds in Methanol Extract of Sweet Star Fruit (Averrhoa carambola L.) Leaves Ni Putu Adriani Astiti; Yan Ramona
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.12

Abstract

The leaves of star fruit plants (Averrhoa carambola) have been traditionally used to cure many diseases, such as hypertension and fever. Besides, the leaves of this plant have also widely been used as the main raw material in lawar (a Balinese traditional food) making. In this research, the methanol extract of this plant leaves was analyzed and identified for active compound contents. The main objective of this research was to investigate types of possible active and applicable compounds contained in such leaves, previously extracted/macerated in methanol solution. Analysis was conducted by applying GC-MS instrumentation using methanol solution. The results showed that 10 possible active and applicable compounds (Butane, 1,1-diethoxy-3 methyl-(CAS)1.1-DII, Dodecanoic acid, methyk ester(CAS) methyl, Dodecanoic acid, methyl ester (CAS) Ethyl Laun, Pentadecanoic acid ethyl ester, Hexadecanoic acid methyl ester (CAS) Methyl pa, OCTADECA 9.12 DIENOIC ACID METHYL, 9-Octadecenoic acid methyl ester (E)-(CAS), Octadecanoic acid methyl ester, and (E) 9-Octadecanoic acid ethyl ester) were identified in methanol extract of such leaves. Among those compounds, Butane, 1,1-diethoxy-3-methyl and Hexadecanoic acid, methyl ester was two most abundance constituents with percentage of peak areas of 35.67% and 26.93%, respectively.
(RESISTANCE OF Lactobacillus spp. FBB TO SIMULATION OF UPPER PART INTESTINAL TRACT CONDITION FOR PROBIOTIC DEVELOPMENT) Ida Ayu Sri Sinca Maha Uni; Yan Ramona; I Nengah Sujaya
ARCHIVE OF COMMUNITY HEALTH Vol 2 No 2 (2013): Desember (2013)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

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Abstract

Lactobacillus spp. FBB (isolates 60, 72, dan 75), isolated from feces of healthy infants, were tested for resistance in a modified gastric juice in vitro, before being applied in in vivo tests. These isolates were exposed for 7 hours in the modified gastric juice (added with pepsin, pancreatin, and NaDC) with various pH of 2, 3, 4, or 8. Isolates of FBB that showed the most resistant properties in this test was identified by applying molecular method or by sequencing its 16S rDNA. In this test, the FBB isolates showed various degree of resistance to the modified gastric juice conditions. FBB72 was found to be more resistant than the two other isolates. This isolate appeared to have the ability to reach colon (viable when reach colon), although its density was very low (104 CFU/ml and 103 CFU/ml at pH 4 and 3, respectively). Based on its 16s rDNA nucleotide sequence, this FBB72 was found to be closely related to Lactobacillus rhamnosus GG ATTCC53103 with 99% similarity.
EFEK KONSUMSI BIO-YOGHURT DARI LACTOBACILLUS RHAMNOSUS SKG34 TERHADAP LIPID PROFILE PADA MANUSIA I Desak Putu Kartika Pratiwi; Komang Ayu Nocianitri; Wayan Redi Aryanta; Yan Ramona; I Dewa Made Sukrama; I Nengah Sujaya
ARCHIVE OF COMMUNITY HEALTH Vol 2 No 1 (2013): Juni (2013)
Publisher : Program Studi Sarjana Kesehatan Masyarakat Universitas Udayana Berasosiasi Dengan Ikatan Ahli Kesehatan Masyarakat Indonesia (IAKMI)

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Abstract

This research was carried out to elucidate the eff ect of consumption bio-yoghurt produced byLactobacillus rhamnosus SKG34 on plasma lipid profi les in human. The study was performed usingpre and post analysis. Eleven healthy volunteers aged between 30-48 yeras old were participatedin this study. Each volunteers consumed 100g of bio-yoghurt containing L.rhamnosus SKG34 for 21 days. Blood samples were taken before the start of experiment and after 21 days of comsumption. Total cholesterol was measured using CHOP-POD method, while triglyceride determinedusing glycerol method, LDL and HD determined using homogenous method. Results of thestudy revealed that consumption of the bio-yoghurt lower values of plasma lipid concentrationsin hypercholesterolemia subjects. Total cholesterol, triglyceride and LDL was reduced within4.91%, 13.84%, and 8.24%, respectively, when compare to its concentrations before treatment.However, the plasma lipid concentrations in normal subjects were almost stable during thetreatment periods. Consumption of bio-yoghurt for 21 days had no eff ect on HDL-cholesterollevel. These results indicate that consumption of bio-yoghurt containing L.rhamnosus SKG34specifi cally lowered the concentrations of serum total cholesterol, LDL, and triglyceride onhypercholesterolemia subjects.
Perubahan Golongan Darah Dan Identifikasi Bakteri Yang Berperan Dalam Merubah Golongan Darah Bagas Susilo; I Ketut Junitha; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p13

Abstract

Blood is one of the most important component of human body. In a case of crime, blood found at the site can be used as an evidence to track back the victim or the person who did such criminal. The main objective of this research were to investigate the time needed for the convertion of blood types and to identify bacterial species with capability to convert blood types. This research was conducted at Forensic Laboratory and Microbiology Laboratory, Faculty of Mathematics and Science, Udayana University. The methods used for detection of blood types and identification of Bacterias were elution method and vitex method, respectively. The results showed that blood type A on iron surface and ceramic media was changed into fake O type on day 25, while blood types of B and AB on ironic media were converted into fake type of O after 30 days of exposure. Two species bacteria (Rhizobium radiobacter and Serratia ficaria) with ability to convert blood type were identified in this research. Key Word: Rhizobium radiobacter, Serratia ficaria, changes of blood types
Eksplorasi Dan Identifikasi Mikroba Pada Rhizosfer Tanaman Stroberi (Fragaria x ananassa Dutch.) Di Kawasan Pancasari Bedugul Gustu Widi Putra; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p09

Abstract

The main objective of this research was to isolate and identify microbes residing in the rhizosphere zone of strawberry (Fragaria x ananassa Dutch.), cultivated at Pancasari village, Bedugul Bali. This was an explorative and descriptive research conducted from April to July 2018. Soil samples were collected from the rhizosphere of strawberry plants and microbes residing in them were isolated by applying dilution and platting method, purified, and identified at the Food Microbology Laboratory, Faculty of Agricultural Technology, Udayana University. Important characteristics of the isolated microbes were compared to those specified in references, such as Bergey’s Manual of Determine Bacteriology 9th Edition (Holt et al., 1994) for bacterial isolates and Fungal Identification keys (Barnett and Hunter, 1972; dan Pitt and Hocking, 2009) for fungal isolates. Microbes successfully isolated and identified in this research included fungi and bacteria belong to Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, and Colletotrichum sp., Pseudomonas sp., and Bacillus sp. Keywords: rhizosphere, strawberry, microbes.
Hambatan in Vitro Cendawan Antagonis pada Fusarium sp., Penyebab Penyakit pada Tanaman Buah Naga (Hylocereus undatus (Haw.) Britton & Rose) Dewa Ayu Andriastini; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p14

Abstract

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.
Potensi Ekstrak Ethanol Daun Salam Dan Air Rebusan Daun Salam Untuk Memperbaiki Profil Lipid (Ldl-Kolesterol) Darah Pada Tikus Wistar Yulidia Iriani; Yan Ramona; Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p09

Abstract

Hyperlipidimia merupakan suatu kelainan yang memicu terjadinya penyakit kardiovascular yang jumlah penderitanya cenderung mengalami peningkatan. Kelainan ini disebabkan oleh pola makan masyarakat yang cenderung mengkonsumsi makanan tinggi lemak, tetapi rendah kadar serat. Pemakaian obat paten, seperti simvastatin merupakan pilihan utama saat ini untuk mengatasi masalah tersebut. Namun, pemakaian obat ini dalam waktu lama tidak dianjurkan karena dapat menyebabkan efek samping merugikan bagi tubuh, sehingga perlu dicari bahan alam yang mempunyai efek sama dengan obat tersebut. Pada penelitian ini, potensi hypolipidemia daun salam diuji pada tikus jantan galur Wistar karena secara empirik daun tanaman ini sering dipakai sebagai obat tradisional. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak etanol daun salam (EEDS) dan rebusan daun salam (ARDS) terhadap menurunkan LDL-Kolesterol tikus galur Wistar. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan delapan kelompok perlakuan yaitu perlakuan dengan aquades (kontrol negatif), simvastatin 0,36 mg/kgBB (kontrol positif), EEDS 252mg/kgBB (EEDS 252), 504mg/kgBB (EEDS 504), 756mg/kgBB (EEDS 756), ARDS 25%/kgBB (ARDS 25), 50% mg/kgBB (ARDS 50), dan 75% mg/kgBB (ARDS 75). Perlakuan diberikan selama satu minggu pada tikus yang sebelumnya diberi pakan kaya lemak selama satu bulan. Hasil penelitian menunjukkan terjadinya penurunan kadar LDL-Kolesterol secara signifikan (p?0.05) pada semua kelompok perlakuan dengan kisaran potensi sebesar 8,00±0,82% sampai 56,16 ± 5,69% (< kontrol negatif dengan penurunan sebesar 6,25 ± 0,50%). Bahkan pada perlakuan 756mg/kgBB EEDS (EEDS 756) menunjukkan hasil yang lebih baik (p?0.05) daripada kontrol positif (simvastatin). Efek hypolipidemia dari ekstrak daun salam ini diduga disebabkan oleh golongan senyawa-senyawa flavonoid, alkaloid, dan tannin yang terkandung dalam ekstrak daun salam. Kata kunci: Daun salam, LDL-Kolesterol, Tikus Wistar
IDENTIFIKASI ANTAGONIS DARI Xanthomonas campestris YANG DIISOLASI DARI RHIZOSPHERE PERKEBUNAN BROKOLI (Brassica oleracea var. italica) DI DESA KEMBANG MERTA, KABUPATEN TABANAN, BALI Nadya Treesna Wulansari; Yan Ramona; Meitini Wahyuni Proborini
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The main objectives of this research were to isolate and identify antagonists of Xanthomonas campestris from rhizosphere zone of broccoli plants. Soil samples were collected from broccoli farm located at Kembang Merta village, Tabanan, Bali. Isolation and identification of the antagonists were conducted at the Laboratory of Microbiology, Udayana University. Two fungal (Trichoderma harzianum and  Trichoderma viride) and two bacterial (Bacillus sp. and Pseudomonas sp.) antagonists potentially to be developed as biocontrol agents of Xanthomonas campestris were successfully identified in this research.
ISOLATION, SCREENING, AND CHARACTERIZATION OF PROBIOTICS (LACTIC ACID BACTERIA) ANTAGONISTIC AGAINST Candida albicans Ida Ayu Ketut Ariningsih; Yan Ramona; Nyoman Semadi Antara
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p19

Abstract

Candidacies in female reproductive tract are mainly caused by Candida albicans. This infection often causes serious problems, particularly on their reproductive tract (genital part). Until recently, control of this infection has relied on the use of antibiotics. However due to numerous bad side effects of antibiotics, lactic acid bacteria have been proposed as an alternative method to control the growth of Candida albicans. Therefore, this research was aimed to isolate, screen, and characterize lactic acid bacterial isolates (LAB) antagonistic against Candida albicans (the causative agent of candidacies infection in reproductive tract of human). LABs were isolated from various fermented foods, such as tape ketan and kimchi. Isolation of LABs was conducted by applying dilution and spread plate method on MRS agar medium supplemented with BCP indicator to distinguish LABs from non acid-producing bacteria. Colonies with indication to produce acid were screened for antagonistic activity against C. albicans on MRS agar and followed by characterization of those isolates (Gram stain, catalase production test, oxydase production, gas production test, resistance test to low pH conditions and to high level of NaDC (sodium deoxicolic), and test for ability to convert colic acid (CA) into deoxicolic acid (DCA)). The results showed that 46 LAB isolates were successfully isolated from samples of tape ketan and kimchi. Among those, 7 isolates showed antagonistic activity against C. albicans in in vitro tests. All these 7 candidates were also found to be resistance to low pH conditions (up to pH 2) and to high level of NaDC (up to 0.6 mM). Four most potential isolates were further testes for ability to convert colic acid into deoxycolic acid and none showed positive result, indicating that they all showed initial potential and safe for future human probiotic development (especially to be used to treat patients infected by C. albicans).
Mekanisme Kerja Antibiotik Ceftriaxone, Clarithromycin, dan Senyawa Aktif Quercetin pada Streptococcus pneumoniae dengan Metoda Docking In Silico Irma Damayanti; Yan Ramona; Sentot Joko Raharjo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p01

Abstract

Pneumolysin is a protein that plays an important role in cell death and bacterial colonization of Streptococcus pneumoniae. Ceftriaxone, clarithromycin, dan quercetin has been proven (in vivo and in vitro) to prevent the action of pneumolysin. The purpose of this study was to identify interactions between ceftriaxone, clarithromycin, and quercetin in inhibiting the pneumolysin activity through in silico. The 3D structure of pneumolysin was downloaded via RCSB PDB (PDB ID:PQQA), while 3D structure of ceftriaxone (ID:5479530), clarithromycin (ID:84029), and quercetin (ID:5280343) were downloaded via PubChem. Protein preparations were done with Discovery Studio, while ligands were prepared with OpenBabel. The docking process used HEX 8.0.0 and visualization analysis with Discovery Studio. Pneumolysin-ceftriaxone interaction produces 4 hydrogen bonds, 5 hydrophobic bonds, 1 electrostatic bond, and 2 unfavorable residue bonds with a bond energy value -329.98kJ/mol. Pneumolysin-clarithromycin interaction produces 3 hydrogen bonds, 1 electrostatic bond, and 3 unfavorable residue bonds with a bond energy value -311.12kJ/mol. Pneumolysin-quercetin interaction produces 2 hydrogen bonds, 4 hydrophobic bonds, and 2 electrostatic bonds with a bond energy value -246.52kJ/mol. Keyword: pneumolysin, ceftriaxone, clarithromycin, quercetin, in silico.
Co-Authors A. Line, Martin A.A. Ketut Darmadi Agestiawan, I. G. A. M. Agung Panji Dwipayana, I Dewa Alayna Himawan Anak Agung Gede Indraningrat Anak Agung Ketut Darmadi Anak Agung Ngurah Nara-Kusuma Ananda Sri Phartiswari Andriani, Luh Nyoman Ary Metta Anissa Maria Hidayati Ayu Amelia Bagas Susilo Batho, Yanuarius Felix Cok. Istri Dewiyani Cakrawati Cokorda Istri Sri Arisanti Dandy Bimantara Dewa Ayu Andriastini Dewa Ayu Swastini Diah Kharismawati Djereng Esti Arisetya Dewi Fainmarinat Selviani Inabuy Fernando Putra Flavia Fionessa Falenda Shanzet Fukuda Kenji Gusti Ayu Dianti Violentina Gustu Widi Putra Hani, Ni Luh Helda Thorikul Affan I D.P. Kartika Pratiwi I Dewa Gede Cahyadi Kusuma I Dewa Made Sukrama I G. A. Widagda I Gusti Agung Gde Bayu Wirama I Gusti Ngurah Jemmy Anton Prasetya I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Oka Adi Parwata I Made Saka Wijaya I N.K. Widjaja I Nengah Sujaya I Putu Aditya Prayoga I Putu Oka Suartama I W BUDIARSA SUYASA I Wayan Suanda I Wayan Suardana I. M. A Sasmitha I.B. Oka Suyasa I.D.P.K. Pratiwi IBG Darmayasa Ida Ayu Ketut Ariningsih Ida Ayu Putu Suryanti Ida Ayu Sri Sinca Maha Uni Ida Bagus Gede Darmayasa Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara Inabuy, Fainmarinat selviani Irma Damayanti K.R Suciptha Kadek Wegi Kurnilia KOMANG AYU NOCIANITRI Komang Dharmawan Kusuma, I Dewa Gede Cahyadi Meitini Proborini Wahyuni N. N. Rupiasi N.G.A Manik Ermayanti N.W.A. Utami Nadia Khairunnisa Nadya Treesna Wulansari Ni LUH ARPIWI Ni Luh Made Ema Juliasari Ni Luh Putu Ariwathi Ni Luh Suriani Ni Made Susilawati Ni Made Teriyani Ni Made Utami Dwipayanti Ni Nengah Dwi Fatmawati Ni Nyoman Rupiasih Ni Putu Adriani Astiti Ni Putu Desy Aryantini Ni Putu Eka Leliqia Ni Putu Indiani Sahaning Karna Ni Putu Linda Laksmiani Ni Putu Suariani Ni Putu Widarini Ni Putu Widiantari Ni Wayan Eka Putri Gayatri Kastawa Ni Wayan Nursini NYOMAN SEMADI ANTARA Pande Gde Sasmita Julyantoro Pande Putu Ayu Sukmawati Putu Eka Sudaryatma Putu Rima Sintyadewi Retno Kawuri SANG KETUT SUDIRGA Sentot Joko Raharjo Septiari, I Gusti Ayu Agung Shetty, Kalidas Sri Wahyuni Sumartin N.W.E. Tadashu Urashima Teriyani, Ni Made Viviandari, I D. A. A Wayan Redi Aryanta Wulandari, Kristina Yenni Ciawi Yoshitake Orikasa Yuji Oda Yulidia Iriani Yulidia Iriani Yustiantara, Putu Sanna Zulfa, Rozifatul