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All Journal The Indonesian Biomedical Journal Jurnal Kesehatan Tambusai Indonesian Journal of Pharmacology and Therapy
Emilia Vivi Arsita
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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GSTM1 Deletion Compensated in mRNA Expression and 4T1 Viability After Editing Using CRISPR/Cas9 Single and Double gRNA Emilia Vivi Arsita; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa
The Indonesian Biomedical Journal Vol 15, No 2 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i2.2131

Abstract

BACKGROUND: Glutathione S-transferase Mu-1 (GSTM1) is known to undergo polymorphism and plays role in drug metabolism including Paclitaxel (PTX), the first-line chemotherapy for breast cancer. However, the effect of GSTM1 polymorphism against chemotherapy in breast cancer is limited and unexplored. This study was conducted to explore the effects of single and double guide (gRNA) on the GSTM1 knocked out (KO) and its effect on the response of PTX in the 4T1 cell line.METHODS: The preparatory stage was done by culturing and electroporating 4T1 cells using Ribonucleoprotein of clustered regularly interspaced short palindromic repeats (CRISPR)/Caspase 9 (Cas9). KO validation was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR), Sanger sequencing, and ICE analysis. The 4T1 viability was examined by MTT Assay.RESULTS: The number of base pairs of GSTM1 after being engineered by single or double gRNA was 86 bases. The DNA quantity of GSTM1 engineered by gRNA was more than using double gRNAs. The mRNA expression of GSTM1 engineered by single gRNA was lower than using double gRNAs. IC50 values of PTX between wildtype and KO were not significantly different, in the range of 30 µM.CONCLUSION: The base-pair length of GSTM1 exon 4 that is knocked out with single and double gRNA have the same number of base pairs. The quantity of GSTM1 DNA and mRNA expression are contrary between single gRNA and double gRNA, and IC50 PTX values in the 4T1 cell line of the control group with single or double gRNA knocked out do not differ markedly. PTX efficiency as chemotherapy is not disturbed in the GSTM1 deletion genetic profile.KEYWORDS: GSTM1, gRNA, Paclitaxel, CRISPR, breast cancer
DAMPAK POLUSI UDARA DARI TRANSPORTASI DARAT TERHADAP KESEHATAN Faradila, Firda Ayu; Sinanto, Rendi Ariyanto; Nursetiawan, Nursetiawan; Axmalia, Astry; Arsita, Emilia Vivi
Jurnal Kesehatan Tambusai Vol. 6 No. 3 (2025): SEPTEMBER 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v6i3.46759

Abstract

Polusi udara merupakan masalah kesehatan lingkungan pada beberapa daerah di Indonesia. Polusi udara tertinggi terjadi di sekitar jalan raya, salah satu jenis polusi udara adalah asap dari kendaraan transportasi darat yang mengandung timbal. Polusi udara dapat berdampak negatif pada kesehatan dalam berbagai bentuk, seperti sesak napas, pusing, kematian, dan lain-lain. Penelitian ini bertujuan untuk mengetahui faktor-faktor apa saja yang mendukung dampak kesehatan, apa saja jenis penyakit yang ditimbulkan dan apa penanganan dari polusi udara. Metode dalam penelitian ini yaitu menggunakan literature review pada database portal garuda yang diakses pada tanggal 29 Januari 2025 sampai dengan 31 Maret 2025 dengan pelaporan naratif. Berdasarkan hasil penelusuran terdapat 33 artikel yang masuk kriteria inklusi. Hasil dalam penelitian ini didapatkan bahwa transportasi darat menghasilkan partikel halus, karbon monoksida, dan nitrogen dioksida yang mengganggu kualitas udara dan berimplikasi pada buruknya kesehatan manusia. Penyakit seperti gangguan pernapasan, penyakit jantung adalah beberapa contoh penyakit jangka panjang yang disebabkan oleh polusi udara. Penanganan yang dapat dilakukan untuk mengatasi persoalan tersebut adalah optimalisasi penggunaan transportasi umum, penggunaan knalpot modifikasi dan sosialisasi. Optimalisasi penggunaan transportasi umum, knalpot modifikasi, dan sosialisasi diperlukan untuk mengatasi polusi udara.
Liposome as carrier therapy for Alzheimer’s disease Anggraeni, Andi Sri Dewi; Arsita, Emilia Vivi; Suwarman, Adika; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 3 No 2 (2022)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.2315

Abstract

Alzheimer's disease (AD) is the most common type of dementia. Approximately 50 million people suffer from Alzheimer's and it is expected to increase more than 100 million by 2050. According to the Diagnostic and Statistical Manual of Mental Disorders V (DSM-V), the diagnosis of AD has an onset of memory impairment and cognitive decline. Effective treatment of AD is a major challenge in the field of pharmacy and science due to the restriction from blood brain barrier (BBB). This causes poor access to pills or oral administration. Liposome as a part of nanoparticle showed to be a potential AD treatment because of its biocompatibility, flexibility, and capability to carry various therapeutic molecules across the BBB and into brain cells. Several types of liposomes provide varied therapy effect. Some factors which determine liposomes effectiveness are its structure, mechanism of action; formed; and delivery, and its preparation method. This article reviewed liposomes characteristics which has potency as carrier therapy for AD.
Co-Authors Adika Suwarman Ahmad Hamim Sadewa Andi Sri Dewi Anggraeni Anggraeni, Andi Sri Dewi Axmalia, Astry Dwi Aris Agung Nugrahaningsih Faradila, Firda Ayu Mustofa Mustofa Mustofa Nursetiawan Nursetiawan, Nursetiawan Sinanto, Rendi Ariyanto Suwarman, Adika