Garuda - Garba Rujukan Digital

Genetically Modified Feed Crops and Feed Ingredients in Indonesia: Opportunities and Constraints of Development Prawiradiputra, Bambang R; S, Muharsini
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 4 (2013)
Publisher : Indonesian Animal Sciences Society

The opportunity of the presence of genetically modifiedÂ organism (GMO) Â forageÂ crops inÂ Indonesia is quite large. Although until now there is no single forage crop awarded safely crop in Indonesia, but several crop byproducts have been used as feed ingredient. The controversy over the presence of GMO plant cannot be avoided. There are a part of communities who could not accept the presence of GMO crops for some reasons. On the other hand, the producers claimed the advantages of the GMO crops such as reducing pesticide application, reducing cost of weeding, more tolerant to biotic and abiotic stresses, and increasing production, farmerâs income and welfare. For the opponent, the main concerns are environmental issues and the possibility of emerging diseases in animal as well as human being. The Biosafety Comission through Biosafety Technical Team has the authority to recommend whether GMO food or feed (and plants) is safe or not safe to be consumed and grown in Indonesia after the assessment. Â Key words: GMO, forage crops, feed safety

Evaluation of mating behaviour and mating compatibility methods for the Old World screwworm fly, Chrysomya bezziana. Wardhana, April H.; MM, Cameron; S, Muharsini; MJR, Hall
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 4 (2013)
Publisher : Indonesian Animal Sciences Society

The effectiveness of the Sterile Insect Technique program (SIT) to eradicate pest insects relies on the success of mating competitiveness between irradiated male flies and wild type males for the wild type females. It has been successfully applied for the New World screwworm fly (NWSF), Cochliomyia hominivorax but remains unproven for the Old World screwworm fly (OWSF), Chrysomya bezziana. The aim of the study was to develop methods for investigating mating behaviour and mating compatibility of C. bezziana under laboratory conditions. Two methods were used for studying mating: individual mating (method 1) and group mating (method 2). The flies used in this study were 5-7 days old. Twenty four hours after emergence, adult flies were sexed and placed into different cages until studied. The female : male ratio in the group mating was 1 : 5 and the males were marked by painting a dot on the thorax using different oil colours. Observation of mating behaviour was investigated every 30 minutes through 10-20 replications for all methods depending on the availability of flies. Data were analysed using ANOVA and the Studentâs t-test, with significance demonstrated at the 95% confidence level. The results demonstrated that the frequency of contacts between males and females at different ages was a significantly different (p < 0.05) and that the duration of copulation was not significantly affected by fly age both method 1 (p > 0.05) and method 2 (p > 0.05). Copulation was only initiated following longer periods of contact, mainly in the range of 270-449 seconds. The highest frequency of copulation occurred between 7-8 days, but the duration of mating was similar between 5-8 days old. The study demonstrated that the methods developed were suitable for a mating compatibility study of C. bezziana. Key Words: Chrysomya bezziana, Mating Behaviour, Copulation, Myiasis

Efficacy of micro-encapsulated of local isolate B. thuringiensis as bio-insectiside for control of myiasis caused by Chrysomya bezziana larvae S, Muharsini; AH, Wardhana
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

B. thuringiensis produces toxic crystal proteine (ï¤-endotoksin) which is specific for insect target, but non-toxic to human or non-target organism. Local isolate of B. thuringiensis have been collected from Kediri Regency where endemic area of myiasis. The aim of this study was to formulate the micro-encapsulated of B. thuringiensis using Freund Incomplete Adjuvant (FIA) for protecting crystal in the field and enviromentally friendly. The formulae was then tested in vitro and in vivo. The result of in vitro trial towards seven local isolates of B. thuringiensis showed a decreased in the toxicity of those several isolates. In vivo trial of nine thin tail sheep using chosen isolat of 45.5A and 47.3A, resulted is no significant different for treatment sheep compared to control sheep (P > 0.05). It is concluded that micro-encapsulation method need to be developed according to the way of living the C. bezziana larvae in animal tissues. Key Words: Chrysomya bezziana, Bacillus thuringiensis, Freund Incomplete Adjuvant, Micro-encapsulated

Efficacy of micro-encapsulated of local isolate B. thuringiensis as bio-insectiside for control of myiasis caused by Chrysomya bezziana larvae Muharsini S; Wardhana AH
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

B. thuringiensis produces toxic crystal proteine (-endotoksin) which is specific for insect target, but non-toxic to human or non-target organism. Local isolate of B. thuringiensis have been collected from Kediri Regency where endemic area of myiasis. The aim of this study was to formulate the micro-encapsulated of B. thuringiensis using Freund Incomplete Adjuvant (FIA) for protecting crystal in the field and enviromentally friendly. The formulae was then tested in vitro and in vivo. The result of in vitro trial towards seven local isolates of B. thuringiensis showed a decreased in the toxicity of those several isolates. In vivo trial of nine thin tail sheep using chosen isolat of 45.5A and 47.3A, resulted is no significant different for treatment sheep compared to control sheep (P > 0.05). It is concluded that micro-encapsulation method need to be developed according to the way of living the C. bezziana larvae in animal tissues. Key Words: Chrysomya bezziana, Bacillus thuringiensis, Freund Incomplete Adjuvant, Micro-encapsulated

Evaluation of mating behaviour and mating compatibility methods for the Old World screwworm fly, Chrysomya bezziana. April H. Wardhana; Cameron MM; Muharsini S; Hall MJR
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 4 (2013): DECEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The effectiveness of the Sterile Insect Technique program (SIT) to eradicate pest insects relies on the success of mating competitiveness between irradiated male flies and wild type males for the wild type females. It has been successfully applied for the New World screwworm fly (NWSF), Cochliomyia hominivorax but remains unproven for the Old World screwworm fly (OWSF), Chrysomya bezziana. The aim of the study was to develop methods for investigating mating behaviour and mating compatibility of C. bezziana under laboratory conditions. Two methods were used for studying mating: individual mating (method 1) and group mating (method 2). The flies used in this study were 5-7 days old. Twenty four hours after emergence, adult flies were sexed and placed into different cages until studied. The female : male ratio in the group mating was 1 : 5 and the males were marked by painting a dot on the thorax using different oil colours. Observation of mating behaviour was investigated every 30 minutes through 10-20 replications for all methods depending on the availability of flies. Data were analysed using ANOVA and the Student’s t-test, with significance demonstrated at the 95% confidence level. The results demonstrated that the frequency of contacts between males and females at different ages was a significantly different (p < 0.05) and that the duration of copulation was not significantly affected by fly age both method 1 (p > 0.05) and method 2 (p > 0.05). Copulation was only initiated following longer periods of contact, mainly in the range of 270-449 seconds. The highest frequency of copulation occurred between 7-8 days, but the duration of mating was similar between 5-8 days old. The study demonstrated that the methods developed were suitable for a mating compatibility study of C. bezziana. Key Words: Chrysomya bezziana, Mating Behaviour, Copulation, Myiasis

Genetically Modified Feed Crops and Feed Ingredients in Indonesia: Opportunities and Constraints of Development Bambang R Prawiradiputra; Muharsini S
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 4 (2013): DECEMBER 2013
Publisher : Indonesian Center for Animal Research and Development

The opportunity of the presence of genetically modified organism (GMO) forage crops in Indonesia is quite large. Although until now there is no single forage crop awarded safely crop in Indonesia, but several crop byproducts have been used as feed ingredient. The controversy over the presence of GMO plant cannot be avoided. There are a part of communities who could not accept the presence of GMO crops for some reasons. On the other hand, the producers claimed the advantages of the GMO crops such as reducing pesticide application, reducing cost of weeding, more tolerant to biotic and abiotic stresses, and increasing production, farmer’s income and welfare. For the opponent, the main concerns are environmental issues and the possibility of emerging diseases in animal as well as human being. The Biosafety Comission through Biosafety Technical Team has the authority to recommend whether GMO food or feed (and plants) is safe or not safe to be consumed and grown in Indonesia after the assessment. Key words: GMO, forage crops, feed safety

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search