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All Journal Bioscientist : Jurnal Ilmiah Biologi
I Gusti Ayu Sri Andayani
Fakultas MIPA Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Ekstrak Daun Jati (Tectona grandis) Alternatif Pewarna pada Penghitungan Jumlah dan Viabilitas Sel Kultur Dibandingkan dengan Pewarna Tryphan Blue I Gusti Ayu Sri Andayani; Sri Sulastri; Dwi Ampera Hananto; Made Sriasih
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 2 (2020): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i2.3015

Abstract

Tryphan blue comes from toluidine which is one of the bases of toluene isomer which has a natural pigment content of anthsiasin. Tryphan blue is commonly used for cell counting and for the calculation of tissue viability in laboratory mice. Basically the use of tryphan blue is dangerous for laboratory workers because it has a carcinogenic effect, mutagenesis, so other materials are needed especially safer and harmless natural materials. This study aims to compare the quality of teak leaf dye extract as a natural dye on the calculation of the number and viability of culture cells compared to tryphan blue dye. The study used the Complete Randomized Design (RAL) method with three treatment factors (96% ethanol, 14% citric acid, and Phospat Buffer Saline (PBS)) and observations of 5 replays be used for the calculation of the number and viability of spleen cells in the 1st, 10th, 20th, and 30th minutes with tryphan blue as a positive control. The results showed that the quality of teak leaf dye with ethanol solvent 96% viability does not differ materially, provides good color quality and obtained the same viability time as tryphan blue.
Performa Rapid Diagnostik Tes Hepatitis B dengan ELISA sebagai Gold Standar I Gusti Ayu Sri Andayani; Mohammad Rizki; Made Sriasih; Ika Nurfitria Tauhida
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9035

Abstract

The ELISA (Enzyme Linked Immunoassay) method has high sensitivity and specificity, qualitatively and quantitatively detecting Hepatitis B Surface Antigen (HBsAg) levels, but the process is long, expensive and requires special expertise, compared to the Rapid Diagnostic Test (RDT) as a rapid test which is more practical and cheaper. The aim of this study was to compare the sensitivity and specificity of the Entebe Rapid Test Diagnostic (ERTD) produced by PT. Hepatika Mataram, with ELISA as the gold standard in detecting HBsAg. The requirement for interpreting sensitivity and specificity results according to World Health Organization (WHO) standards is a minimum of 95%. Testing with a negative panel of 00 samples and a positive panel of 1 samples from Papua in 012 at -80℃ storage. Testing in April 023 at the Molecular Diagnostics and Research Laboratory, Mataram University Hospital. Statistical analysis using MedCal software. ERD HBsAg produced by PT. Hepatika Mataram has a sensitivity of 95.45%, specificity of 99.50%, positive predictive value of 93.59%, negative predictive value of 99.65%, and accuracy of 99.21%.
Optimization of Cryogenic Grinding as a Hair Sample Preparation Technique for Heavy Metal Concentration Analysis I Gusti Ayu Sri Andayani; Indah Retnowati; Baiq Mariana; N Ismillayli; Ardiana Ekawanti; Rahmah Dara Ayunda
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.18503

Abstract

This study aimed to evaluate the effectiveness of cryogenic grinding as a hair sample preparation technique and to compare heavy metal analysis results obtained using Energy Dispersive X-ray (EDX) and Atomic Absorption Spectrophotometry (AAS). Hair samples were collected from five residents of Sekotong and analyzed for Cd, Hg, and Pb content. Data were analyzed descriptively and compared using the Friedman test. The results indicated that AAS consistently yielded higher metal concentrations than EDX. Cd concentrations measured by AAS, EDX of powdered hair, and EDX of intact hair were 0.00656 mg/kg, 0.00130 mg/kg, and 0.00010 mg/kg, respectively. For Hg, the corresponding measurements were 0.00380 mg/kg, 0.00212 mg/kg, and 0.00362 mg/kg, whereas Pb exhibited the largest disparity, with 0.12930 mg/kg for AAS, 0.00224 mg/kg for EDX of powdered hair, and 0.06978 mg/kg for EDX of intact hair. The high variability, particularly in Pb measurements, suggests that heterogeneity in metal distribution and surface contamination affect EDX readings of intact hair. Cryogenic grinding yielded a more homogeneous particle distribution, resulting in EDX measurements of powdered hair that were more representative and numerically closer to AAS results than measurements of intact hair. Although differences were not statistically significant (p > 0.05), these findings support the use of cryogenic grinding to enhance the accuracy of hair-based heavy metal analysis. Furthermore, this method has the potential to reduce the use of destructive chemicals and hazardous waste, making it a safer and more sustainable alternative for community biomonitoring in areas affected by heavy metal contamination.
Co-Authors Ardiana Ekawanti Baiq Mariana Dwi Ampera Hananto Ika Nurfitria Tauhida Indah Retnowati Made Sriasih Mohammad Rizki N Ismillayli Rahmah Dara Ayunda Sri Sulastri