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Tingkat Pengetahuan Publik (Public Knowledge) Terhadap Penyakit Hewan Menular Strategis Rabies Dalam Upaya Mendukung Status Lombok Bebas Rabies Made Sriasih; Sulaiman Ngongu Depamede; Muhammad Ali; Wayan Wariata; Answar Rosyidi
Jurnal Gema Ngabdi Vol. 2 No. 3 (2020): Jurnal Gema Ngabdi
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v2i3.101

Abstract

Rabies is one of strategic infectious animal diseases that attacks the central nervous system. Rabies is very widespread and is found in nearly 150 countries and regions on all continents, except Antarctica. Although West Nusa Tenggara is one of the nine provinces in Indonesia that is free of rabies, the number of dog bite victims reported on the island of Sumbawa with 32 samples of rabies-carrying animal brains identified as positive in early 2019 indicates the need to increase awareness of rabies transmission to Lombok Island. Increasing public knowledge which includes parents and students at primary school, elementary school and high school about rabies through surveys, counseling and demonstration is one of the efforts to prevent and control rabies. The results showed that the extension activities were able to increase participants' knowledge. High school students had the best level of knowledge (97.5% ± 2.08) when compared to the elementary school participants (93% ± 3.56) and elderly respondents (88.75% ± 4.79) of all indicators of knowledge about rabies. The combination of extension activities with hands-on practice has had a positive effect on increasing public knowledge about rabies
Ekstrak Daun Jati (Tectona grandis) Alternatif Pewarna pada Penghitungan Jumlah dan Viabilitas Sel Kultur Dibandingkan dengan Pewarna Tryphan Blue I Gusti Ayu Sri Andayani; Sri Sulastri; Dwi Ampera Hananto; Made Sriasih
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 2 (2020): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i2.3015

Abstract

Tryphan blue comes from toluidine which is one of the bases of toluene isomer which has a natural pigment content of anthsiasin. Tryphan blue is commonly used for cell counting and for the calculation of tissue viability in laboratory mice. Basically the use of tryphan blue is dangerous for laboratory workers because it has a carcinogenic effect, mutagenesis, so other materials are needed especially safer and harmless natural materials. This study aims to compare the quality of teak leaf dye extract as a natural dye on the calculation of the number and viability of culture cells compared to tryphan blue dye. The study used the Complete Randomized Design (RAL) method with three treatment factors (96% ethanol, 14% citric acid, and Phospat Buffer Saline (PBS)) and observations of 5 replays be used for the calculation of the number and viability of spleen cells in the 1st, 10th, 20th, and 30th minutes with tryphan blue as a positive control. The results showed that the quality of teak leaf dye with ethanol solvent 96% viability does not differ materially, provides good color quality and obtained the same viability time as tryphan blue.
Penggunaan Probiotik Dan Magot Untuk Mereduksi Lalat di Peternakan Ayam Pedaging Muara Selayar Desa Pijot Muhamad Ali; Made Sriasih; Anwar Rosyidi; I Wayan Wariata; Muh. Aidil Fitriyan Fadjar Suryadi; Nefi Andriana Fajri; Sulaiman Ngongu Depamede
Jurnal Gema Ngabdi Vol. 4 No. 2 (2022): JURNAL GEMA NGABDI
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v4i2.253

Abstract

Chicken manure waste is one of the main problems for large-scale broiler cultivation, especially in areas close to residential areas. The accumulation of manure that accumulates under the cage attracts the arrival and development of the flies in the cultivation site. The development of these flies will spread to the houses of residents who live around the cage. As a result, many farmers stop cultivating the broilers because of public complaints. To overcome this problem, the use of probiotics from acetic acid-producing bacteria can be used to reduce the development of flies because of the ability of the bacteria to reduce the pH of the media and create an uncomfortable environment for egg growth. Likewise, the development of maggot can be a competing medium that beats the flay. In community service activities regarding "The use of probiotics and maggot production to reduce flies in the Muara Selayar broiler farm, Pijot Village, Keruak District, East Lombok Regency". Through this activity, the development of flies can be emphasized by the use of probiotics and the development of magot which can be used as chicken feed.
IDENTIFIKASI PENYEBAB KEMATIAN DAN KEKERDILAN PADA AYAM RAS PEDAGING DI KELOMPOK PETERNAK MUARA SELAYAR DESA PIJOT KECAMATAN KERUAK KABUPATEN LOMBOK TIMUR Made Sriasih; Sulaiman Ngongu Depamede; Wayan Wariata; Muhamad Ali; Anwar Rosyidi; Aini Aini
Jurnal Abdi Insani Vol 10 No 2 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i2.488

Abstract

Success in broiler farming depends on several factors, namely feed, day-old chick (DOC), poultry management, and disease attacks. The Muara Selayar broiler farmer group in Pijot village in the Keruak sub-district of East Lombok is one of the non-industrial poultry farmers. The problems faced by the Muara Selayar farmer group are the mortality rate of DOC, which reached 7.4% (above the 5% threshold), and stunting syndrome. This community service activity aimed to identify the causes of the problems faced by the group so that losses experienced by farmers could be minimized. This community service activity was carried out through direct identification of dead chickens (post-mortem identification), surveys, and discussions. Based on the identification results, the cause of death of chickens in the Muara Selayar group was coccidiosis, characterized by reddish feces and bleeding in the cecum. Stunting syndrome in livestock is mainly caused by poor quality (grade) of seeds from the partners. The results of the discussion activities conducted with the farmers showed that they need bargaining power with partners about the quality of the DOC, so it is necessary to have special communication with partners who cooperate with them. Farmers must pay attention to good husbandry practices such as sanitizing flocks and the surrounding environment and treating livestock with safe materials that do not cause drug residues. From this community service activity, it can be concluded that coccidiosis was the cause of the death of chickens in the Muara Selayar farmer group. However, other poultry diseases such as Pullorum, ND, and CRD should receive attention because they often appear during the rearing period based on farmer reports. Stunting syndrome is mainly caused by low DOC quality.
Antioxidant, Protein, and Fat of Chicken Nuggets with the Addition of Goat Gelatin H. Hasma; Muhammad Ali; Made Sriasih; Anwar Rosyidi; Ratmawati Malaka
Chalaza Journal of Animal Husbandry Vol 8, No 1 (2023): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/chalaza.v8i1.1931

Abstract

This study aims to determine the antioxidant, protein, and fat content of chicken nuggets through the addition of goat skin and bone gelatin which was previously pretreated with Lactobacillus plantarum 1UHCC and acetic acid. Data were processed using a completely randomized design (CRD) and tested further using Duncan's test with treatments including chicken nuggets with the addition of skin gelatin through Lactobacillus plantarum pretreatment (CGSP), chicken nuggets with the addition of skin gelatin through acetic acid pretreatment (CGSA), chicken nuggets with the addition of bone gelatin through Lactobacillus Plantarum pretreatment (CGBP), chicken nuggets with the addition of bone gelatin through acetic acid pretreatment (CGBA) and control (without adding gelatin), the treatment was repeated 4 times. The results of this study were the highest antioxidant in chicken nuggets, namely with the addition of L. plantarum pretreatment gelatin, which was 20.82%, while the protein content was 57.58%, while the highest fat content was in chicken nuggets with the administration of acetic acid pre-treated goat bone gelatin (CGBA) 3.41%. This study concluded that chicken nuggets with the addition of L. plantarum gelatin pretreatment contained higher antioxidants than the control.
Performa Rapid Diagnostik Tes Hepatitis B dengan ELISA sebagai Gold Standar I Gusti Ayu Sri Andayani; Mohammad Rizki; Made Sriasih; Ika Nurfitria Tauhida
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9035

Abstract

The ELISA (Enzyme Linked Immunoassay) method has high sensitivity and specificity, qualitatively and quantitatively detecting Hepatitis B Surface Antigen (HBsAg) levels, but the process is long, expensive and requires special expertise, compared to the Rapid Diagnostic Test (RDT) as a rapid test which is more practical and cheaper. The aim of this study was to compare the sensitivity and specificity of the Entebe Rapid Test Diagnostic (ERTD) produced by PT. Hepatika Mataram, with ELISA as the gold standard in detecting HBsAg. The requirement for interpreting sensitivity and specificity results according to World Health Organization (WHO) standards is a minimum of 95%. Testing with a negative panel of 00 samples and a positive panel of 1 samples from Papua in 012 at -80℃ storage. Testing in April 023 at the Molecular Diagnostics and Research Laboratory, Mataram University Hospital. Statistical analysis using MedCal software. ERD HBsAg produced by PT. Hepatika Mataram has a sensitivity of 95.45%, specificity of 99.50%, positive predictive value of 93.59%, negative predictive value of 99.65%, and accuracy of 99.21%.