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FRANS SUYATNA
UNIVERSITAS INDONESIA
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Karakterisasi Senyawa Utama Ekstrak Daun Aglaia elliptica Blume dan Uji Sitotoksisitas pada Sel Kanker Payudara AGUNG ERU WIBOWO; FRANS SUYATNA; WAHONO SUMARYONO; NURYATI CHAERANI SIREGAR
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 2 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

AIglaia elliptica Blume is one of plant species from the genus of Aglaia, belonging to the family of Meliaceae. This group of plants is known to have powerful cytotoxic effect. This research was conducted to isolate and characterize major compound(s) from ethanol extract of Aglaia elliptical leaf that can be used as marker compound(s) for extract standardization. Approximately 1000 g of Aglaia elliptica Blume leaf dry powder was extracted with ethanol using maceration method. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Six sub-fractions were obtained from the Vacuum Liquid Chromatography (VLC) process of ethyl acetate fraction. Based on the HPLC profile and separation process using column chromatography and preparative HPLC, a major compound (with the weight of 36.80 mg) was successfully isolated from sub-fraction H. Analyses on its UV absorbance pattern, mass spectra, and proton NMR identified the isolated compound as a compound with molecular weight of 300, C18H24N2O2, known as odorin. Cytotoxicity test showed that odorin has inhibition effect against MCP-7 cell line with IC5O of 153.32 ppm.
Aktivitas Biji Klabet (Trigonella foenum-graecum L.) terhadap Pertumbuhan dan Apoptosis Sel Kanker Payudara MCF-7 KURNIA AGUSTINI; FRANS SUYATNA; NURJATI CHAIRANI SIREGAR; WAHONO SUMARYONO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Fenugreek seed (Trigonella foenum-graecum L.) is one of Indonesian medicinal plant with many known activities such as antidiabetic, antihypercholesterolemia, phytoestrogen and anticancer. Fenugreek seed contains steroidal sapogenines (i.e. diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside), alkaloid trigonellin and flavonoid (i.e vitexin, orientin). This experiment was carried out to investigate the cytotoxic activity and its potency to induce apoptotic on breast cancer cell line, MCF-7. Cytotoxic activity was carried out by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) method. Fenugreek seed was prepared by extraction (methanolic and ethanolic extract) and fractionation using solvents with different polarity (hexane, ethylacetic, buthanolic and water fractions). The potency of apoptotic induction assay was carried out using flowcytometric method staining with propidium iodide (20μg/mL) on 100 ppm sample. Apoptotic potency was evaluated based on % population of cells in subG0/G1 phase. Results showed that ethylacetic fraction has the lowest IC50 (41.81 ppm) and significantly (p<0.05) increase % population of cells in subG0/G1 phase (20.87%) on 100 ppm concentration. It is concluded that fenugreek seed posses cytotoxic activity and potency of apoptotic induction on MCF-7 cells.
Co-Authors AGUNG ERU WIBOWO KURNIA AGUSTINI Nurjati Chairani Siregar NURYATI CHAERANI SIREGAR Wahono Sumaryono