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All Journal Microbiology Indonesia Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Enrichment : Journal of Management Lumbung Farmasi : Jurnal Ilmu Kefarmasian Medical Sains : Jurnal Ilmiah Kefarmasian
Shirly Kumala
Universitas Pancasila
Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Isolation and Screening of Endophytic Microbes from Morinda citrifolia and their Ability to Produce Anti-Microbial Substances SHIRLY KUMALA; ENDRO BUDI SISWANTO
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.23 KB) | DOI: 10.5454/mi.1.3.9

Abstract

Assaying for, and isolation and screening of endophytic microbes from the Morinda citrifolia plant and their ability to produce anti-microbial substances was carried out. Endophytic microbes are microorganisms that live asymptomatically within the living tissue of host plants. Microorganisms such as bacteria, fungi, and yeast can be associated with the host and produce secondary metabolites. These secondary metabolites may be enzymes and other bioactive substances with medicinal activity such as anti-arthritic, anti-cancer, and anti-microbial compounds. The aims of these experiments was to investigate the ability of endophytic microbes isolated from M. citrifolia to produce secondary metabolites which can act as anti-microbial agents. A direct seed inoculating technique was used by planting the plant sample onto the surface of Nutrient Agar and Potato-Dextrose Agar. Assessment of their ability to produce anti-microbial substances was conducted by growing the endophyte isolates in Muller Hinton Broth for bacterial isolates, and in Potato-Dextrose Yeast Broth for fungal isolates. The agar diffusion method using paper disk was applied to assay the anti-microbial activity of each substance. The results of the endophyte isolation in these experiments gave five bacterial isolates and eleven fungal isolates. All of the bacterial isolates showed a broad antimicrobial spectrum while ten of the fungal isolate demonstrated broad anti-microbial activity and four out of the ten fungal isolates had activity towards Candida albicans.
FORMULASI GEL KOMBINASI EKSTRAK KERING LIDAH BUAYA (Aloe vera. (L) brum. f.) DAN EKSTRAK KENTAL DAUN SIRIH MERAH (Piper crocatum ruiz & pav) UNTUK ANTIBAKTERI PENYEBAB JERAWAT . Suhaimi; Teti Indrawati; Shirly Kumala
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 3 No 2 (2019)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.397 KB) | DOI: 10.37874/ms.v3i2.67

Abstract

Ekstrak kering lidah buaya dan ekstrak kental daun sirih merah mengandung senyawa alkaloid, flavonoid, tanin, saponin dan fenol yang mempunyai aktivitas sebagai antibakteri penyebab jerawat. Penelitian ini bertujuan untuk memformulasi sediaan gel antibakteri penyebab jerawat kombinasi ekstrak lidah buaya dan ekstrak kental daun sirih merah yang stabil secara fisik, kimia dan mikrobiologi serta memiliki efektivitasnya. Formulasi gel kombinasi esktrak kering lidah buaya dan ekstrak kental dan sirih merah sebanyak 4 formula menggunakan gelling agent, carbophol serta bahan tambahan lainnya. Sediaan yang dihasilkan dievaluasi dan diuji aktivitasnya. Evaluasi sediaan meliputi pemeriksaan organoleptis, homogenitas, uji kemampuan menyebar, viskositas, sifar alir, pH dan uji mikrobiologi. Uji aktivitas meliputi pertumbuhan terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus. Hasil evaluasi didapatkan formula terbaik yaitu formula 1 yang mengandung 1,56 % kombinasi ekstrak kering lidah buaya dan ekstrak kental daun sirih merah kemudian di uji stabilitas pada suhu kamar (25-30oC) dan suhu 60oC selama 3 minggu. Hasil uji stabilitas menunjukan tidak ada perbedaan yang signifikan selama penyimpanan.
Isolasi dan Uji Antimikroba Substansi Bioaktif Mikroba Endofit Ranting Tanaman Johar (Cassia siamea Lamk.) Shirly Kumala; Syarmalina Syarmalina; Atut Ruswita Handayani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Assay for secondary metabolite of endophytic fungi from Cassia siamea Lamk.. (Johar Plant) has been investigated. Endophyitic fungi are microorganisms that live asymtomatically within the living tissue of host plants. Endophytic fungi associate with plant, they can help the metabolism of host plant and produce potensial secondary metabolite. The purpose of this experiment were to investigate endophytic microbial agents. Isolate of endophytic fungi from twig of Cassia siamea Lamk., using direct seed method was carried out. The microbial activity was carried out with dilution method using paper disk and glass cylinder. The results showed 3 isolates of bacteria and 7 isolates of endophtyic fungi. The metabolite from three isolates of bacteria had the anti-microbial activity more poten toward Gram positive than Gram negative and four isolates of fungi had anti-Aspergillus agent.
Kapang Endofitik Tanaman Brucea javanica (L.) Merr yang Berpotensi menghasilkan Enzim Xilanase Wibowo Mangunwardoyo; Shirly Kumala; I. G.A. Ayu Wiratih
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Xylanase enzyme activity of endophytic fungi from Brucea javanica (L.) Merr has been done. Fruit, leave and stem of the plant collected from Bogor, Cianjur, and Tawangmangu which used in this study. To evaluate the enzyme activity the endophytic fungi isolate were inoculated on PDY medium and fermented at room temperature, shake at 150 rpm for 14 days. The supernatan were used to examine the extracellular enzyme and the biomass for intracellular enzyme. The DNS methods was used to test the enzyme activity. The results showed isolate 1.1.8; 1.2.17; 1.3.16 and 1.3.6 produced intra and extracellular xylanase enzyme. Isolate 1.1.6 produced only extracellular enzyme while isolate 1.2.2 did not produce both intra and extracellular enzyme.
Fermentasi Diam dan Goyang Isolat Kapang Endofit dari Brucea javanica L. Merr. dan Uji Aktivitas Antimikroba Shirly Kumala; Wibowo Mangunwardoyo; Peny Budiarti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Exploring an antibiotic from microorganism is potential to discover the new antibiotic. The endophytic microorganism is one of the minimanufacturer producing the secondary metabolite such as antibiotic. Screening on the endophytic molds isolated from Brucea javanica L. Merr has been carried out. Fermentation in still and shake culture were used to produce the antibiotic using PDY medium. Antimicrobial activity was assayed using diffusion agar method. The results revealed that shake culture gave a better antimicrobial activity than still culture. Amount of eight isolate studied showed microbial activity against Gram positive bacteria: Staphylococcus aureus and Bacillus subtilis also Gram Negative bacteria: Escherichia coli and Salmonella typhi, however no microbial activity recorded againts Candida albicans. Isolate number 1.2 gave the highest antimicrobial activity on still culture and isolate number 1.7 gave the highest antimicrobial activity on shake culture. Further research should be carried out on identification and purification of the antimicrobial agent.
Uji Aktivitas Enzim Xilanase Ekstraselular dan Intraselular Bakteri Endofitik Tanaman Brucea javanica (L.) Merr. Shirly Kumala; Wibowo Mangunwardoyo; Diah Dethriani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 2 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.241 KB)

Abstract

Assay for xylanase enzyme activity of endophytic bacteria from Brucea javanica (L.) Merr. has been carried out. Parts of the plants used in this study were stem, leave and fruit. Samples were collected from Bogor, Cianjur and Tawangmangu. To evaluate the enzyme activity the fungi were shakefermented for 14 days. The supernatant were used to examine the extracellular enzyme and biomass for intracellular enzyme, using DNS methode to test the activity of enzyme. The results showed that from ten isolates only four isolates produced xylanase enzyme. Isolate 2.3.1 and 2.3.6 produced extra and intracellular enzyme, while isolate 2.1.15 and 2.3.13 only produced extracellular xylanase enzyme.
Penapisan Kapang Endofit Ranting Kayu Meranti Merah (Shorea balangeran Korth.) sebagai Penghasil Enzim Xilanase SHIRLY KUMALA; NUR ANNISA FITRI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 1 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Endophytic microbes are microorganisms that live in the plant. These microbes, are able to produce secondary metabolites, such as oligosaccharide degrading enzymes, growth factors, antibacterial substances and Xylanaze enzyme, an enzyme that can reduce the use of chlorine as bleaching agent in paper industries. This study Was aimed to isolate endophytic fungi that can produce extracellular xylanaze. Isolation of endophytic fungi from red memnti (Shorea balangeran Korth.) branches was carried out using surface sterilization and direct seeding methods on CMM (Corn Meal Malt) medium. Xylanaze was produced by using shaking fermentation methods on PDY (Potato Dextrose Yeast) for 12 days. Enzyme activity was assessed by using DNS (Dinitro salicylic acid) method. The results showed that of nine endophytic fungi isolates, eight have xylanaze enzyme activity, ranging from 1.279 µ/ml (highest) to 0.12 µ /ml (lowest).
Efek Imunostimulan Ekstrak Etanolik Herba Pegagan (Centella asiatica (L.) Urban) terhadap IgG Mencit Jantan Shirly Kumala; Aulia Tisna Dewi; Yun Astuti Nugroho
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The immunostimulating effect of an ethanolic extract of Pegagan herbs to male mice has been determined. Thirty male mice divided into five groups (P1-P5) were induced by 1% sheep red cells for 14 days. P1, P2 and P3 were treated with three doses of an ethanolic extract 4.124 mg/20g BW, 8.25 mg/20g BW and 16.5mg/20gBW respectively. P4 was a positive control using Stimuno 0.55 mg/20g BW, and P5 was a negative control using distilled water Blood samples were taken at day 1, 8 and 15, and the concentration of IgG was measured by Radial Immunodiffusion method. A two way Anova showed no significant difference between P1, P2, and P5, but P3 was higher than P4. The obtained results showed that the ethanolic extract of 16,5 mg has a potent immunostimulating effect.
Produk Esktraseluler Isolat Kapang Endofi t C.1.1 dan C.3.3 dari Ranting Cempaka Kuning (Michelia champaca L.) Sebagai Antimikroba Adefira Elisyia Juniharti; Desi Nadya Aulena; Novi Yantih; Nur Miftahurrohmah; Shirly Kumala
JURNAL ILMU KEFARMASIAN INDONESIA Vol 19 No 1 (2021): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v19i1.985

Abstract

Michelia champaca L. is known to have effi cacy as an antimicrobial, antioxidant, and anticancer.In general, endophytic fungi that live in plant tissues is capable of producing secondary metabolites as theirhost plant. The objective of this research is to examine the antimicrobial potential of endophytic mold isolates C.1.1 and C.3.3 from yellow cempaka branches (Michelia champaca L.). Isolates obtained from previous research, fi rst was rejuvenated on PDA. Fermentation was carried out on each isolate for 12 days. The fermented supernatant was then extracted partially with n-hexane, ethyl acetate, and n-butanol solvent. The extract was then concentrated and tested for antimicrobial activity using disk diff usion and microdilution methods. N-butanol extract of endophytic fungi isolates of yellow champaca branches was able to inhibit the microbial growth with the largest inhibition area in isolate C.1.1 in Staphylococcus aureus ATCC 6538 (12.26 mm), Escherichia coli ATCC 8739 (11.40 mm), Candida albicans ATCC 10231 (10.50 mm), and Metichillin-Resistant Staphylococcus aureus (11.30 mm) with Minimum Inhibitory Concentration and Minimum Concentration of 12,5% . It was concluded that both endophytic fungi isolates of yellow champaca branches had the potential to inhibit the growth of pathogenic microbes.
Sinergisme Fraksi Butanol Metabolit Sekunder Kapang Endofit 1.3.11 dengan Doxorubicin dalam Modulasi Daur Sel T47D dan MCF-7 SHIRLY KUMALA; EDY MEIYANTO; MUTHI IKAWATI; RIRIS ISTIGHFARI JENIE
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The synergic effects of n-butanolic fraction of secondary metabolite of endophytic fungus 1.3.11 (FB) and doxorubicin (Dox) on cell cycle regulation and the expression of Bcl-2 gene expression were investigated on MCF-7 and T47-D cells by iiow cytometry and immunocytochemical techniques respectively. The results showed that after 12 hours of incubation period with FB at its IC50 dose, MCF-7 cell cycle was inhibited at G1 phase while Dox inhibited the cell cycle at G2/M phase. Similar results were observed in T47-D cells when incubated with Dox and FB individually under the same treatment condition. Further treatment was then performed to these cells where both Dox and FB were combined at their IC50 and lC50 dose and added to incubate with the cells over 12 hours period. Interestingly, the modified treatment combination showed that MCF-7 and T47-D cell cycle regulation were inhibited at G2/M phase. Our immunocytochemical study also showed no significant inhibition suppression of Bel-2 gene expression in both MCF-7 and T47-D cells when compared with their corresponding positive control after treatment with FB and Dox or both combined FB and Dox at 1C50 and IC50 dosage over 15 hours incubation.
Co-Authors . Suhaimi Adefira Elisyia Juniharti Afrini Saraswati AMEILIA AMEILIA Anarisa Budiati Anny Victor Purba Atut Ruswita Handayani Aulia Tisna Dewi Desi Nadya Aulena Diah Dethriani Dimas Adrianto Dimas Adrianto EDY MEIYANTO ENDRO BUDI SISWANTO I. G.A. Ayu Wiratih Indah Masri Meva Sari Chandra Miftahurrohmah, Nur Muthi Ikawati Noviyantih, Noviyantih NUR ANNISA FITRI Partomuan Simanjuntak Peny Budiarti RIRIS ISTIGHFARI JENIE Sesilia Andriani Keban Simanjuntak, Partomuan Siswa Setyahadi Sofyan Ramani Syarmalina Syarmalina Teti Indrawati Teti Indrawati Wibowo Mangunwardoyo Yonathan Tri Atmodjo Reubun Yun Astuti Nugroho