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All Journal Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Jurnal Ilmu dan Teknologi Kesehatan Health Media
Mega Mirawati
Poltekkes Kemenkes Jakarta III
Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Health Professions Public Health

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Tingkat Cemaran Salmonella pada Minuman Es Cappucino Cincau yang Dijual di Wilayah Pondok Gede–Bekasi Husjain Djajaningrat; Mega Mirawati; Heru Setiawan
Jurnal Kesehatan Vol 6, No 2 (2015): JURNAL KESEHATAN
Publisher : Politeknik Kesehatan Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.427 KB) | DOI: 10.26630/jk.v6i2.100

Abstract

Es Capucino cincau merupakan olahan es kopi yang manis dan segar dengan tambahan cincau yang justru memberi tekstur unik namun tidak asing. Cincau merupakan jenis gel nabati yang mempunyai kandungan karbohidrat tinggi, biasa digunakan sebagai bahan campuran minuman . Namun pada proses pengolahan Es cappucino cincau dapat mengalami kontaminasi bakteri patogen contohnya adalah Salmonella sp yang bila tertelan dalam jumlah banyak oleh manusia maka dapat menganggu kesehatan. Menurut Peraturan Kepala BPOM, batas maksimum cemaran Salmonella sp. dalam es , susu, cincau adalah negatif. Penelitian ini bertujuan untuk mengidentifikasi adanya kontaminasi Salmonella pada minuman es cappucino cincau yang dijual di wilayah Pondok Gede Bekasi. Penelitian deskriptif dengan pengambilan sampel random sampling yang berada di sekitar wilayah Pondok Gede Bekasi, metoda yang digunakan biakan/kultur. Hasil penelitian menunjukkan bahwa dari 32 sampel, sebesar 9.37% sampel yang terkontaminasi Salmonella sp. sedangkan 40,63% sampel terkontaminasi bakteri  lain dan 50.0% sampel tidak terkontaminasi bakteri. Kesimpulan bahwa ditemukan minuman es cappucino yang terkontaminasi Salmonella paratiphi  A. Kata Kunci: Minuman Es Cappucino Cincau, Salmonella sp.
KUALITAS BAKTERIOLOGIS CABAI GILING YANG DIJUAL DI PASAR TRADISIONAL WILAYAH PONDOK GEDE Mega Mirawati; Husjain Djajaningrat; Angki Purwanti
Jurnal Ilmu dan Teknologi Kesehatan Vol 1 No 1 (2013): September
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (33.141 KB)

Abstract

Milled chili is the result of milling fresh chili, with or without the preservatives. In the manufacture of milled chili, there's so many milled chili processing that use rot chili as the raw materials. it can lead contamination by the bacteria that cause of food poisoning. According to ISO 2011, the maximum limit of microbial contamination in seasoning include milled chili is Salmonella sp negatif/25g, Staphyllococcus aureus 1x102 colonies / g and MPN Coliform 100/g. This research aims to determine the bacteriological quality of milled chili which are sold in the traditional markets at Pondok Gede area and the percentage of milled chili with good quality. This research is experimental research with milled chili samples are sold in traditional markets at Pondok Gede area. this research was done at the Laboratory of Bacteriology Department Analis Kesehatan Poltekkes Kemenkes Jakarta III. the methods were used are culturing and the Most Probable Number (MPN). The result of 32 samples milled chili, was found 11 samples with a good quality because there is not overgrown by the bacteria and 21 samples are not in good quality because were found the bacteria was growing with the amount exceeds the maximum bacteria contamination limits which specified in ISO. The raw materials, water for washing chili, packaging, sanitation, and the use of preservatives affect the quality of milled chili. Conclusion that not all milled chili are sold in traditional markets at Pondok Gede area have good quality and the percentage of milled chili with good quality is 34,375% and not good quality is 65,625%
PENGARUH PEMBERIAN KARBOL FUCHSIN DAN PEMANASAN SPUTUM SEBELUM PEMBUATAN SEDIAAN TERHADAP HASIL PEWARNAAN BTA Mega Mirawati; Estu Lestari
Jurnal Ilmu dan Teknologi Kesehatan Vol 5 No 1 (2017): September
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (757.96 KB) | DOI: 10.32668/jitek.v5i1.59

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. This disease is a health problem in the community. Minimal checks that need to be done to confirm pulmonary tuberculosis diagnostics are smear examinations. ZiehlNelseen method is an inspection method recommended by WHO. Sputum used to make preparations is a mucopurulent sputum. This can lead to infected laboratory workers if inhaled droplets containing Mycobacterium tuberculosis. This study aims to determine the effect of fuchsin carbolic acid and sputum heating before the preparation of the smear result. Temperature used 60oC, 70oC, 80oC and 90oC and control is sputum staining with Ziehl Neelsen method. The samples used positive smear sputum. The experimental research design and data were analyzed with Kruskal Wallistest. The research was conducted at the Bacteriology Laboratory of Medical Laboratory Technology Poltekkes Kemenkes Jakarta III in February to October 2016. The result of statistical test showed p> 0,301 which means there was no significant difference between the dyeing result by using sputum which has been given carbolic fuchsin and heating before the preparation with the result staining method ZiehlNeelsen. The conclusion of this method can coloring Mycobacterium tuberculosis bacteria.
IDENTIFIKASI SALMONELLA PADA JAJANAN YANG DIJUAL DI KANTIN DAN LUAR KANTIN SEKOLAH DASAR Mega Mirawati; Estu Lestari; Husjain Djajaningrat
Jurnal Ilmu dan Teknologi Kesehatan Vol 1 No 2 (2014): Maret
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (30.492 KB)

Abstract

Child street food has become part of everyday children, especially elementary school. Snack food purchased in a less assured of cleanliness can lead to diseases of the gastrointestinal tract due to bacterial contamination . One of the bacteria that contaminate food are Salmonella. According to ISO 2011, the maximum contaminant limit of Salmonella in foods is negatip/25g. This study aimed to identify the presence of Salmonella contamination on foods sold in the elementary school at Pondok Gede. This research is descriptive . Samples of food and drinks sold in elementary school of Pondok Gede . The study was conducted at the Laboratory of Bacteriology of Analis Kesehatan Poltekkes Kemenkes Jakarta III in October-November 2013. The method use in this research was culture . The study of 28 samples of food found 10 samples ( 35.7 % ) contaminated with salmonella. A total of 4 samples ( 26.7 % ) came from the canteen and 6 samples ( 46 % ) from outside the canteen. The results indicate identification of 4 samples (40 %) contaminated with Salmonella typhi and 6 samples ( 60 % ) contaminated with Salmonella paratyphi A. Presence of Salmonella can be caused due to the influence of raw materials , water , presentation , container and environmentalhygiene.Conclusion that street food is found contaminated with Salmonella typhi and Salmonella paratyphi A.
POLA KEPEKAAN KUMAN TERHADAP ANTIBIOTIKA DI RUANG RAWAT INAP ANAK RUMAH SAKIT KANKER DHARMAIS JAKARTA TAHUN 2014 Mega Mirawati; Estu Lestari; Demak L Tobing
Jurnal Ilmu dan Teknologi Kesehatan Vol 3 No 1 (2015): September
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.281 KB)

Abstract

Antibiotic resistance is becoming a public health threat in Indonesia, then to controlgerms that are immune surveillance is needed periodically. Patterns germs andsensitivity test results can be used as the basis for empirical antibiotic. This studyaims to determine the sensitivity pattern of bacteria to antibiotics in inpatient childrenDharmais Cancer Hospital Jakarta in 2014. This study is a descriptive study usingsecondary data sensitivity test results of bacteria to antibiotics in inpatient childrenDharmais Cancer Hospital in 2014. Results culture showed that the type of pathogensfound in January-June 2014 was Pseudomonas aeruginosa (14.99%), Esherichia coli(13.89%), and in July - December 2014 pathogenic germs found were Acinetobacterbaumannii (16, 25%) and Escherichia coli (15.35%). Of the 990 isolates obtainedsensitivity pattern of germs showed the sensitivity of Gram positive to ceftazidime(100%), Trimethoprim (100%), Linezolid (100%), Cefuroxime (100%), Amikacin(100%), Netilmicin (100%), Vancomycin (100%) and Tigercycline (100%). Gramnegativebacteria highest sensitivity towards Doripenem. Gram-positive bacteriahighest resistance to ampicillin (100%) and Moxifloxacin (100%). Gram-negativegerms showed the highest resistance to Amoxicillin results (100%). The conclusionthat the Gram-positive bacteria are sensitive to ceftazidime, Trimethoprime,Linezolid, Cefuroxime, Amikacin, Netilmicin, Vancomycin and Tigercycline andGram-negative bacteria sensitive to Doripenem.
Relationship Between CD4+ Cell with GenExpert Molecular Mycobacterium tuberculosis Test in HIV/AIDS patients Al’fira Gita Islami; Rizana Fajrunni'mah; Mega Mirawati
Health Media Vol 3 No 2 (2022)
Publisher : UrbanGreen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55756/hm.v3i2.67

Abstract

CD4+ cells in HIV/AIDS patients is an examination that serves to monitor the regulation of the immune system. HIV/AIDS patients experience a decrease in the number of CD4+ cells which results in various opportunistic infections, one of which is caused by Mycobacterium tuberculosis. This study aims to determine the relationship between the results of CD4+ cell with GenExpert Molecular Mycobacterium tuberculosis Test. This study was based on an analytic observational cross-sectional design on 53 samples of HIV/AIDS patients at Budhi Asih Hospital. Data analyze using Fisher Exact statistical test with α = 0.05. The result showed percentage CD4+ cells <200 with positive TCM result 48 (86,4%). CD4+ cells >200 with GenExpert positif result 0 (0,0%). The results showed that there was no relationship between CD4+ cell examination and TCM GenExpert MTB examination in HIV/AIDS patients (p = 0.574). From the result we can conclude that maybe other infection has happened.
THE EFFECT OF JICAMA (Pachyrhizus erosus) CONCENTRATION ON THE GROWTH OF Staphylococcus aureus BACTERIA IN SOLID MEDIA Mega Mirawati; Tri Prasetyorini; Fania Irmadhani
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.3093

Abstract

Background:Synthetic media for microbial growth are expensive, thus opening the way for making bacterial growth media using local raw materials at low prices. Jicama is one of the local raw materials that can be used as natural media for the growth of microorganisms. It contains a source of carbohydrates needed for the development of microorganisms. Aims: This study aims to determine the effect of variations in the concentration of Jicama, which has the potential to grow Staphylococcus aureus bacteria. Methods: This study used experimental research with the Static Group Comparison method. In this study, the experimental group Staphylococcus aureus was instilled in Jicama solid media with various concentrations (2%, 3%, 4%, 5%, 6%) with five repetitions. The results: The results showed that the average number of Staphylococcus aureus bacteria colonies in jicama flour with a concentration of 2% was 0, a concentration of 3% was 0, a concentration of 4% was 0.8, a concentration of 5% was 1.4, and at a concentration of 6% was 2.2. The results of the Kruskal-Wallis test with a degree of confidence of 0.05 obtained a p-value = 0.000 where the p-value 0.05 so that it can be concluded that there is a significant effect of variations in the concentration of jicama flour on the growth of Staphylococcus aureus bacteria. Conclusions:For future researchers, it is necessary to conduct further research using higher concentrations of jicama flour and by adding other nutrients, such as animal protein, so that the growth of Staphylococcus aureus bacteria is more optimal.
Co-Authors Al’fira Gita Islami Angki Purwanti Demak L Tobing Estu Lestari Estu Lestari Fania Irmadhani Heru Setiawan Husjain Djajaningrat Rizana Fajrunni’mah Tri Prasetyorini