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Uki Dwiputranto
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENGARUH INOKULASI MIKORIZA VESIKULA ARBUSKULA (MVA) CAMPURAN TERHADAP KEMUNCULAN PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum) Uswatun Hasanah; Purnomowati Purnomowati; Uki Dwiputranto
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.748 KB) | DOI: 10.20884/1.sb.2017.4.1.382

Abstract

Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.
PENGARUH PERENDAMAN ETIL METAN SULFONAT (EMS) TERHADAP DAYA TAHAN TANAMAN KECIPIR Psophocarpus tetragonolobus (L.) DC POLONG PENDEK DARI SERANGAN PATOGEN Rhizoctonia solani Hani Khoiril Khasanti; Adi Amurwanto; Uki Dwiputranto
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.995 KB) | DOI: 10.20884/1.sb.2014.1.3.550

Abstract

Induced Mutations with EMS (Ethyl Methane Sulfonate) has been widely reported to increase genetic diversity and improve quality of the crop. This study aimed to find out the effect of EMS immersion on short pod winged bean plant (Psophocarpus tetragonolobus (L.) DC) resistance from Rhizoctonia solani pathogens attack and to determine the optimum concentration to obtain mutan plants which resistant to R. solani pathogen attack. The method was experimental with Completely Randomized Design (CRD). Variables used were 2, i.e. independent and dependent variables. The independent variable was 4 levels of EMS concentrations (E0 = no EMS, E1 = 0,1% EMS, E2 = 0,3% EMS and E3 = EMS 0,5 %) and the dependent variable was the resistance of plants against disease intensity caused by R. solani. Observed parameters were dumping-off disease intensity caused by R. solani. The experiment was replicated 6 times. Data were analyzed using F test with confident levels of 5% and 1%. Furthermore, LSD (Least Significant Difference) test were carried out to determine the differences of each treatment. The results indicated that EMS caused the short pods winged bean plants more vulnerable against the attack of R. solani. Mutants categories obtained in the three treatments of EMS concentrations. Mutants were found in E1, E2 and E3 treatments in the intensity of dumping-off disease of R. solani pathogens in categories 1 and 4; 4; 3 and 4, respectively.
Co-Authors Adi Amurwanto Hani Khoiril Khasanti Purnomowati Purnomowati Uswatun Hasanah