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Adi Amurwanto
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA Arini Maesaroh; Adi Amurwanto; Alice Yuniaty
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.879 KB) | DOI: 10.20884/1.sb.2014.1.1.11

Abstract

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.
PENGARUH PERENDAMAN ETIL METAN SULFONAT (EMS) TERHADAP DAYA TAHAN TANAMAN KECIPIR Psophocarpus tetragonolobus (L.) DC POLONG PENDEK DARI SERANGAN PATOGEN Rhizoctonia solani Hani Khoiril Khasanti; Adi Amurwanto; Uki Dwiputranto
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.995 KB) | DOI: 10.20884/1.sb.2014.1.3.550

Abstract

Induced Mutations with EMS (Ethyl Methane Sulfonate) has been widely reported to increase genetic diversity and improve quality of the crop. This study aimed to find out the effect of EMS immersion on short pod winged bean plant (Psophocarpus tetragonolobus (L.) DC) resistance from Rhizoctonia solani pathogens attack and to determine the optimum concentration to obtain mutan plants which resistant to R. solani pathogen attack. The method was experimental with Completely Randomized Design (CRD). Variables used were 2, i.e. independent and dependent variables. The independent variable was 4 levels of EMS concentrations (E0 = no EMS, E1 = 0,1% EMS, E2 = 0,3% EMS and E3 = EMS 0,5 %) and the dependent variable was the resistance of plants against disease intensity caused by R. solani. Observed parameters were dumping-off disease intensity caused by R. solani. The experiment was replicated 6 times. Data were analyzed using F test with confident levels of 5% and 1%. Furthermore, LSD (Least Significant Difference) test were carried out to determine the differences of each treatment. The results indicated that EMS caused the short pods winged bean plants more vulnerable against the attack of R. solani. Mutants categories obtained in the three treatments of EMS concentrations. Mutants were found in E1, E2 and E3 treatments in the intensity of dumping-off disease of R. solani pathogens in categories 1 and 4; 4; 3 and 4, respectively.
Co-Authors Alice Yuniaty Arini Maesaroh Hani Khoiril Khasanti Uki Dwiputranto