Scripta Biologica
Vol 1, No 3 (2014)

PENGARUH PERENDAMAN ETIL METAN SULFONAT (EMS) TERHADAP DAYA TAHAN TANAMAN KECIPIR Psophocarpus tetragonolobus (L.) DC POLONG PENDEK DARI SERANGAN PATOGEN Rhizoctonia solani

Hani Khoiril Khasanti (Fakultas Biologi, Universitas Jenderal Soedirman)
Adi Amurwanto (Fakultas Biologi, Universitas Jenderal Soedirman)
Uki Dwiputranto (Fakultas Biologi, Universitas Jenderal Soedirman)



Article Info

Publish Date
01 Sep 2014

Abstract

Induced Mutations with EMS (Ethyl Methane Sulfonate) has been widely reported to increase genetic diversity and improve quality of the crop. This study aimed to find out the effect of EMS immersion on short pod winged bean plant (Psophocarpus tetragonolobus (L.) DC) resistance from Rhizoctonia solani pathogens attack and to determine the optimum concentration to obtain mutan plants which resistant to R. solani pathogen attack. The method was experimental with Completely Randomized Design (CRD). Variables used were 2, i.e. independent and dependent variables. The independent variable was 4 levels of EMS concentrations (E0 = no EMS, E1 = 0,1% EMS, E2 = 0,3% EMS and E3 = EMS 0,5 %) and the dependent variable was the resistance of plants against disease intensity caused by R. solani. Observed parameters were dumping-off disease intensity caused by R. solani. The experiment was replicated 6 times. Data were analyzed using F test with confident levels of 5% and 1%. Furthermore, LSD (Least Significant Difference) test were carried out to determine the differences of each treatment. The results indicated that EMS caused the short pods winged bean plants more vulnerable against the attack of R. solani. Mutants categories obtained in the three treatments of EMS concentrations. Mutants were found in E1, E2 and E3 treatments in the intensity of dumping-off disease of R. solani pathogens in categories 1 and 4; 4; 3 and 4, respectively.

Copyrights © 2014






Journal Info

Abbrev

scribio

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and ...