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Slamet Priyanto
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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AKTIVITAS PROTEASE DAN AMILASE PADA HEPATOPANKREAS DAN INTESTINE IKAN NILEM Osteochilus hasselti C.V. Syarifah Fauziah Al Gadri; Untung Susilo; Slamet Priyanto
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.969 KB) | DOI: 10.20884/1.sb.2014.1.1.24

Abstract

Ikan Nilem (Osteochilus hasselti C.V) merupakan ikan omnivora dari familia Cyprinidae. Jenis pakan yang dikonsumsi berkaitan dengan alat pencernaan yang dimilikinya. Pencernaan pada ikan tersebut terjadi di dalam usus halus dan berlangsung secara enzimatis. Enzim-enzim yang aktif pada saluran pencernaannya adalah protease dan amilase yang dihasilkan oleh pankreas dan hepatopankreas. Aktivitas enzim pencernaan diketahui dengan mengukur banyaknya mikromol maltosa dan tirosin yang dihasilkan per menit. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas protease dan amilase pada hepatopankreas dan intestine ikan Nilem (Osteochilus hasselti C.V.). Hasil penelitian ini diharapkan nantinya akan digunakan sebagai dasar formulasi pemberian pakan yang cocok untuk ikan omnivora salah satunya adalah ikan Nilem (Osteochilus hasselti C.V.). Materi yang digunakan adalah ikan Nilem berukuran 9-16 cm dengan bobot 5-50 gram. Metode penelitian yang dilakukan adalah metode survey dengan purposive random sampling dengan rancangan percobaan berupa Rancangan Acak Lengkap (RAL) dengan pola faktorial 3x4 dan empat kali pengulangan. Sampel dikelompokkan menjadi tiga ukuran yang berbeda dengan bobot 5-10 g dan panjang 9-10 cm, 11-25 g dan panjang 11-12 cm dan 35-50 g dengan panjang 13-16 cm. Metode analisis yang digunakan yaitu one way analisis of variance (ANOVA) dan dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil uji BNT menunjukkan bahwa aktivitas protease dan amilase ikan Nilem tidak berbeda nyata (P>0,05) dengan ukuran tubuh ikan. Aktivitas protease dan amilase ikan Nilem berbeda nyata (P<0,05) dengan organ pencernaan. Dengan demikian dapat disimpulkan bahwa tidak ada hubungan antara aktivitas protease dan amilase dengan ukuran tubuh ikan Nilem; serta ada hubungan antara aktivitas protease dan amilase dengan organ pencernaan ikan Nilem.
PENGGUNAAN BEBERAPA MEDIUM SEMISINTETIK UNTUK PRODUKSI MISELIUM JAMUR MAITAKE (Grifola frondosa (Dickson: Fr.) S. F. Gray) ISOLAT CIANJUR DAN EKSTRAK KASARNYA Maria Mardhitama Maharani; Nuniek Ina Ratnaningtyas; Slamet Priyanto
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.828 KB) | DOI: 10.20884/1.sb.2014.1.1.20

Abstract

Research on the use of some semisynthetic medium for the production of fungal mycelium Maitake (Grifola frondosa (dickson: Fr.) SF Gray) isolates Cianjur and crude extract was performed with an incubation period of 30 days. This study aimed to determine the ability of green bean, cowpea and maize as an alternative material of semisynthetic medium for manufacturing G. frondosa’s mycelium and and to know the production of G. frondosa’s mycelium and the highest crude extract. This study was experimental study with a completely randomized design consisted of 4 treatments : Yeast Potato Dextrose Broth (PDYB) medium, Green bean Yeast Dextrose Broth (GbDYB) medium, Cowpea Yeast Dextrose Broth (CpDYB) medium and Corn Yeast Dextrose Broth (CDYB) medium. The highest average dry weight of mycelium (1,584 g/100ml) was GbDYB medium. The lowest average dry weight of the mycelium (g/100ml 0.244) was PDYB medium. The weight of the crude extract of mycelium in each treatment was lower than the dry weight. The highest weight of the crude extract was obtained from the GbDYB medium treatment (1,22 g) and the lowest was obtained from PDYB medium (0,113 g). Anova test results of different treatment was very significant, meaning that the use of extract of green bean, cowpea and maize greatly affected the growth of G.frondosa’s mycelium. The LSD test between treatment of PDYB medium and CDYB medium was not significant, meaning that the increase of mycelium’s growth  on PDYB medium had no different with the CDYB medium.
RESPON AKTIVITAS AMILASE DAN PROTEASE IKAN GURAMI Osphronemus gouramy Lac. TERHADAP PERBEDAAN TEMPERATUR AIR Dwiyan Oktavianto; Untung Susilo; Slamet Priyanto
Scripta Biologica Vol 1, No 4 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.598 KB) | DOI: 10.20884/1.sb.2014.1.4.45

Abstract

Research to determine the activity of amylase and protease digestion gourami on various temperature has been carried out experimentally using a completely randomized design (CRD) with 3 treatments and 4 replications. The treatments were included gourami nurturing on water temperature 24oC (P1), gourami nurturing on water temperature 28oC (P2), and gourami nurturing on water temperature 32oC (P3). The results the amylase activity is showed no significant difference between treatments (P>0,05) where as protease activity showed there are significant differences between treatments (P0≤0,05). In conclusion, the difference in protease activity of gourami occured in water temperature 24oC (P1) and 28oC (P2) and the protease activity was highest at 28oC water temperature, but the amylase activity did not difference.
PRODUKSI MISELIUM Grifola frondosa (Dickson: Fries) Gray ISOLAT CIANJUR DAN BOBOT EKSTRAKNYA PADA MEDIUM MYPB DENGAN PENAMBAHAN BIJI BUNGA MATAHARI Helianthus annuus L. Rizki Maulida; Nuniek Ina Ratnaningtyas; Slamet Priyanto
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.243 KB) | DOI: 10.20884/1.sb.2014.1.1.21

Abstract

Grifola frondosa or maitake, not only can be used as a food ingredient but also as medicine. Other than nutrients, fruit body and mycelium of G. frondosa also contains bioactive compounds, such as terpenoids, alkaloids, flavonoids, and beta glucans extracellular polysaccharides (β-1,3 glucans and β-1,6 glucans). Extracellular polysaccharide harvested more often in mycelium form which is cultivated in liquid medium. Liquid medium which is commonly used for the growth of mycelium is MYPB (Malt Yeast Peptone Broth). Mycelium production on MYPB as a medium can be increased by adding additional ingredients, one of which is sunflower (Helianthus annuus L.). The treatment was the addition of sunflower seeds on medium MYPB. The main parameters were mycelium’s dry weight and the weight of raw extract of G. frondosa. Supporting parameters were final pH medium, extracellular polysaccharides (qualitatively), terpenoid, alkaloid, and flavonoid compounds in the raw extract of G. frondosa. The addition of 250 g/l sunflower seeds in the medium MYPB was the optimum treatment that can produce 1,379 g/100ml of mycelium and 0,299 g/100ml G. frondosa extracts.
Co-Authors Dwiyan Oktavianto Maria Mardhitama Maharani Nuniek Ina Ratnaningtyas Rizki Maulida Syarifah Fauziah Al Gadri Untung Susilo