Scripta Biologica
Vol 1, No 1 (2014)

PENGGUNAAN BEBERAPA MEDIUM SEMISINTETIK UNTUK PRODUKSI MISELIUM JAMUR MAITAKE (Grifola frondosa (Dickson: Fr.) S. F. Gray) ISOLAT CIANJUR DAN EKSTRAK KASARNYA

Maria Mardhitama Maharani (Fakultas Biologi, Universitas Jenderal Soedirman)
Nuniek Ina Ratnaningtyas (Fakultas Biologi, Universitas Jenderal Soedirman)
Slamet Priyanto (Fakultas Biologi, Universitas Jenderal Soedirman)



Article Info

Publish Date
25 Mar 2014

Abstract

Research on the use of some semisynthetic medium for the production of fungal mycelium Maitake (Grifola frondosa (dickson: Fr.) SF Gray) isolates Cianjur and crude extract was performed with an incubation period of 30 days. This study aimed to determine the ability of green bean, cowpea and maize as an alternative material of semisynthetic medium for manufacturing G. frondosa’s mycelium and and to know the production of G. frondosa’s mycelium and the highest crude extract. This study was experimental study with a completely randomized design consisted of 4 treatments : Yeast Potato Dextrose Broth (PDYB) medium, Green bean Yeast Dextrose Broth (GbDYB) medium, Cowpea Yeast Dextrose Broth (CpDYB) medium and Corn Yeast Dextrose Broth (CDYB) medium. The highest average dry weight of mycelium (1,584 g/100ml) was GbDYB medium. The lowest average dry weight of the mycelium (g/100ml 0.244) was PDYB medium. The weight of the crude extract of mycelium in each treatment was lower than the dry weight. The highest weight of the crude extract was obtained from the GbDYB medium treatment (1,22 g) and the lowest was obtained from PDYB medium (0,113 g). Anova test results of different treatment was very significant, meaning that the use of extract of green bean, cowpea and maize greatly affected the growth of G.frondosa’s mycelium. The LSD test between treatment of PDYB medium and CDYB medium was not significant, meaning that the increase of mycelium’s growth  on PDYB medium had no different with the CDYB medium.

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Journal Info

Abbrev

scribio

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and ...