Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
D. A. Santosa
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Transformasi Tanaman Tebu (cv. PSJT 94-41) dengan Gen Fitase Menggunakan Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) *] , Susiyanti; G. A. Wattimena; M. Surahman; A. Purwito; D. A. Santosa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.864 KB) | DOI: 10.24831/jai.v35i3.1333

Abstract

Phosphorus is a critical nutrient for the growth and development of sugarcane in the marginal land in Indonesia. P stored in plant  as phytic acid (myo-inositolhexakisphosphate).  The objective of the study was to increase activity  of phytases enzyme in sugarcanes (cv. PSJT 94-41) through phytase gene transformation. Detection of chimeric gene by PCR showed that the phytase gene was integrated into the genome of sugarcanes. Transformation caused some abnormality such as albino, discoloration, lack of chlorophyll in the particular spot of leaves.  Putative transgenic  plantlets expressed a  higher levels of phytase enzyme activity (38.1 %), whereas increase in P available in plant (19.5 %) and content of  chlorophyll (32.3 %).   Key words: Sugarcane, transformation, phytase,  Agrobacterium tumefaciens
Co-Authors , Susiyanti A. Purwito G. A. Wattimena M. Surahman