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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Sains Dan Teknologi (SAINTEKBU)
Antok Wahyu Sektiono
Brawijaya University
Agriculture, Biological Sciences & Forestry Computer Science & IT Control & Systems Engineering Environmental Science Library & Information Science

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Optimalisasi Fosfat untuk Meningkatkan Pertumbuhan Kerapatan Populasi dan Kemampuan Antagonis Saccharomyces cerevisiae terhadap Fusarium sp. Hardianto -; Anton Muhibuddin; Antok Wahyu Sektiono
SAINTEKBU Vol 10 No 2 (2018)
Publisher : KH. A. Wahab Hasbullah University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977.081 KB) | DOI: 10.32764/saintekbu.v10i2.206

Abstract

Saccharomyces cerevisiae is a common yeast used as a fermenter in the home industry. This yeast is able to grow in media like waste materials. One of the waste materials that can be used as a medium of yeast growth is waste of coconut water. The use of coconut water as a medium of yeast propagation has been widely used in some types of yeasts. The intake of nutrients such as phosphate will make the yeast cells begin to grow and work faster. The yeast cell takes phosphate as ATP. Khamir will turn it into a phosphate polymerization form that is often found within the mitochondria of these cells. S. cerevisiae has the ability not only in terms of fermentation but also can perform other functions in the biological control process. The main methods of this study include the growth test of S. cerevisiae with the addition of a phosphate (KH2PO4), S. cerevisiae growth test by aerator method, yeast antagonist test. The results showed that S. cerevisiae was able to grow higher with the addition of phosphate nutrients (0.5% KH2PO4). This yeast has the potential to control Fusarium sp. The percentage of inhibition was isolate A0 (9,67%), A1 (11%), A2 (10,67%), A3 (12%), A4 (13%), and A5 (6%). Keywords: Yeast, phosphate nutrient, biological control, Fusarium sp.
Yeast from Epiphyte of Avocadoes to Control Colletotrichum gloesporioides Causing Antrachnose Disease Anton Muhibuddin; Siti Fadhilah; Antok Wahyu Sektiono; Umi Kulsum Nur Qomariyah; Mazidatul Faizah; Ambar Susanti; Sri Nurhatika
SAINTEKBU Vol 10 No 2 (2018)
Publisher : KH. A. Wahab Hasbullah University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.552 KB) | DOI: 10.32764/saintekbu.v10i2.208

Abstract

Anthracnose disease caused by Colletotrichum gloesporioides fungi is an important disease in avocado fruit during storage. An effective, cheap, and non-poisoned control method was required during storage to minimize chemical pesticide residues. This observation was aimed to select yeast with antagonistic ability from avocado epiphyte. Selected yeasts then tested their antagonist ability versus Colletotrichum gloesporioides, the fungi causing anthracnose disease, in vitro. Based on visual observation, microscopically and macroscopically, knowing that there were 3 yeast species isolated from green-long avocado variety i.e.: Candida sp., Filobasidium sp., and Cryptococcus sp., and then from mega gagauan avocado variety i.e.: Pichia sp., Candida sp., Cryptococcus sp., and Metschnikowia sp. The most antagonist yeast was Cryptococcus sp, which isolated from green long avocado. Keyword: Yeast, antagonist, storage, avocado, anthracnose
Potential of Wild Yeast from Banana to Control Colletotrichum musae Fungi Caused Anthracnose Disease and Its Short Antagonistic Mechanism Assay Anton Muhibuddin; Antok Wahyu Sektiono; Dewi Maratus Sholihah
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Anthracnose caused by Colletotrichum musae is one of several important disease in banana fruit. An effective, cheap, and safe control method are necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that has antagonist ability effectively in controlling anthracnose disease of banana fruit.  This research was started with an isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from ambon variety was Candida sp. and Pichia sp. Metchnikowia sp. from kepok variety respectively.
Co-Authors Ambar Susanti Anton Muhibuddin Dewi Maratus Sholihah Hardianto - Mazidatul Faizah Siti Fadhilah Sri Nurhatika Umi Kulsum Nur Qomariyah