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Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy herd and human health. The aim of this study is to detect MAP in the growing up milk formula. Fifty samples from five established distributors were taken in Bogor. Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer. Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57. Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia. Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

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Deteksi Mycobacterium Avium Subspesies Paratuberculosis pada Susu Pasturisasi yang Dijual di Bogor Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Ewald Usleber
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Mycobacterium avium subspesies paratuberculosis (MAP) is a thermal tolerant bacteria. The presenceof these bacteria in pasteurized dairy milk is associated with infectious bowel disease in human known asCrohn’s disease. The aim of this study was to detect MAP in pasteurized dairy milk sold in Bogor. Fourtytwo samples of plain flavoured milk (180–250 ml) from 7 producers were bought from supermarkets inBogor. The presence of MAP was detected by isolation and conventional polymerase chain reaction (PCR)using IS 900 and F57. Bacterial isolation were done by Herrold’s egg yolk medium with mycobactine J(HEYMj) and without mycobactin J (HEYM) and incubated at 37°C for 20 weeks. The DNA extraction ofall pasteurized dairy milk samples were conducted by DNeasy® Tissue Kit. Amplification conditionsfor PCR were: 1 cycle at 94°C for 10 minutes, 40 cycles at 94°C for 1 minute, 58°C for 1 minute, and72°C for 3 minutes, and 1 cycle at 72°C for 7 minutes. After 20 weeks of incubation, there were no sign ofMAP which grew in all isolation mediums. The PCR IS 900 and F57 did not detect the DNA band of thetarget. In the conclusion, there was no MAP detected in pasteurized dairy milk sold in Bogor.

Potensi Nyamuk Sebagai Vektor Di Daerah Endemik Filariasis Kabupaten Bogor, Jawa Barat : Potential of Mosquito as Vectors In Filariasis Endemic Area, Bogor District, West Java Nirwan, Muhammad; Upik Kesumawati Hadi; Susi Soviana; Surachmi Setyaningsih; Fadjar Satrija
Aspirator Vol 15 No 1 (2024): Jurnal Aspirator Volume 15 Nomor 1 2024
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58623/aspirator.v15i1.76

Mosquitoe vectors of filariasis must have a long life so that the parasite can complete its life cycle in the mosquito’s body. Mosquitoes with high parity are generally more at risk of transmitting pathogens, as they have made more bites on hosts that have the potential to carry the infection. This study aims to detect the potential of mosquito vectors by calculating the parity number and examining the causative agent in the mosquito’s body. Mosquito parity examination was based on the results of mosquito ovary dissecting during a capture period. Detection of the causative agent was carried out by dissecting tecnique and polymerase chain reaction (PCR). The results showed that the parity rate of mosquitoes caught in Tamansari Village and Cimanggis Village was very high (>80%). Detectionof the causative agent using surgical and PCR methods did not find any L3 larvae and microfilariae in the examined mosquitoes.

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