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All Journal Jurnal Teknologi Dan Industri Pangan Forum Pasca Sarjana Jurnal Sain Veteriner
Ewald Usleber
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Agriculture, Biological Sciences & Forestry Education Industrial & Manufacturing Engineering Veterinary

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Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor Widagdo Sri Nugroho; Minarwati Sudarwanto; Denny Widaya Lukman; Ewald Usleber
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.407 KB)

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to Crohn’s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with John’s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57
Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants.  Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD.  Meanwhile Indonesia imported milk and milk products from those countries to cover national demand.  In the future it will be a potential problem to national dairy herd and human health.  The aim of this study is to detect MAP in the growing up milk formula.  Fifty samples from five established distributors were taken in Bogor.  Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer.  Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57.  Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia.   Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR
Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Abstract

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Co-Authors Abdulwahed A. Hassan Abdulwahed Ahmed Hassan Denny W. Lukman Denny Widaya Lukman Minarwati Sudarwanto MIRNAWATI SUDARWANTO Mirnawati Sudarwanto Rochman Naim Surachmi Setyaningsih Widagdo S. Nugroho Widagdo Sri Nugroho