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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat The International Journal of Tropical Veterinary and Biomedical Research

Widagdo Sri Nugroho

Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Author-ID : 333537

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Nugroho, Widagdo Sri; Sudarwanto, M.; Lukman, D.W; Setiyaningsih, E.; Usleber, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

Johneâs disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. Itâs potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herroldâ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johneâs disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces

Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johneâs disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herroldâs egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57

Analisis Spasial Kasus Leptospirosis di Perbatasan Kabupaten Bantul, Sleman, dan Kulon Progo Nurbeti, Maftuhah; Kusnanto, Hari; Nugroho, Widagdo Sri
Kes Mas: Jurnal Fakultas Kesehatan Masyarakat Vol 10, No 1 (2016): Kes Mas: Jurnal Fakultas Kesehatan Masyarakat
Publisher : Universitas Ahmad Dahlan

Background: Leptospirosis is an important issue in Yogyakarta Province. Outbreak status had set in Sleman, Bantul, and Kulonprogo District. The border areas have the most cases. This study aimed to analyze: cluster based on the buffer of distance from caseâs home to rice fields, rivers, and roads; as well as observed patterns of disease diffusion and frequency over time. Method: This study was an observational study. There were 327 subjects obtained from all cases of Leptospirosis who were reported in District Health Office from 2009-2011. They were living in seven sub-districts, namely Sedayu, Minggir, Moyudan, Godean, Sentolo, Nanggulan, and Kalibawang. Results: 1) Most of the cases were distributed in the agricultural area, area drained by many river streams, and area with moderate rainfall (2000-2500 mm/year). There was no specific distribution pattern in overlay of cases map with maps of population density, density of poor households, altitude, and density of livestock. 2) Buffer analysis showed a cluster based on the distance between cases home with rice fields, rivers, and roads. 3) All cases in the seven sub-district was one unit cluster. The area is the most widely drained by large and small rivers. Cluster scores are higher in areas with the highest deprivation scores and has the most widely watershed. We found some Leptospirosis clusters which were across-district, across sub districts, and across the river. 4) Disease diffusion of Leptospirosis cases was an infectious type of expansion diffusion. There was a seasonal pattern according to the planting season and the beginning of the rainy season. There was a trend of increase in Leptospirosis cases from year to year. Conclusion: Leptospirosis disease in the border of Bantul, Sleman, and Kulonprogo District did not spread through the river from one region to another, but very much related to the watershed.

Background: Leptospirosis is an important issue in Yogyakarta Province. Outbreak status had set in Sleman, Bantul, and Kulonprogo District. The border areas have the most cases. This study aimed to analyze: cluster based on the buffer of distance from case’s home to rice fields, rivers, and roads; as well as observed patterns of disease diffusion and frequency over time. Method: This study was an observational study. There were 327 subjects obtained from all cases of Leptospirosis who were reported in District Health Office from 2009-2011. They were living in seven sub-districts, namely Sedayu, Minggir, Moyudan, Godean, Sentolo, Nanggulan, and Kalibawang. Results: 1) Most of the cases were distributed in the agricultural area, area drained by many river streams, and area with moderate rainfall (2000-2500 mm/year). There was no specific distribution pattern in overlay of cases map with maps of population density, density of poor households, altitude, and density of livestock. 2) Buffer analysis showed a cluster based on the distance between cases home with rice fields, rivers, and roads. 3) All cases in the seven sub-district was one unit cluster. The area is the most widely drained by large and small rivers. Cluster scores are higher in areas with the highest deprivation scores and has the most widely watershed. We found some Leptospirosis clusters which were across-district, across sub districts, and across the river. 4) Disease diffusion of Leptospirosis cases was an infectious type of expansion diffusion. There was a seasonal pattern according to the planting season and the beginning of the rainy season. There was a trend of increase in Leptospirosis cases from year to year. Conclusion: Leptospirosis disease in the border of Bantul, Sleman, and Kulonprogo District did not spread through the river from one region to another, but very much related to the watershed.

Penilaian Organisasi Perangkat Daerah (OPD) tentang Otoritas Veteriner dalam Kebijakan Penanggulangan Rabies di Provinsi Banten Ismadi, Joko; Sumiarto, Bambang; Nugroho, Widagdo Sri; Purwanto, Erwan Agus
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Veterinary Authority (Otovet) is a government institution, formed in order to deal with decision making of anything related to animal health, by involving veterinarians and mobilizing all related professional lines. The purpose of this study was to describe the Regional Organizationsâ€™ (OPD) assessment of the implementation of Veterinary Authority (Otovet) in rabies management policies. Research material included 36 Regional organizations (OPD), 9 Technical services, supervising animal health (keswan) and veterinary public health (kesmavet), 9 Health services, 9 Regional development planning services, 9 Regional staffing services, Banten Province. The results comprised eight questions: 1). Are the organization bureaucratic? 2). Positions, roles, and authority of veterinarians? 3). Is the veterinary authority regarding rabies control already in line with the implementation? 4). Strategic policy 5). Cooperations OPD, 6). Regional Policy 7). Veterinarian openings, andÂ 8). Enforcement and improvement of otovet institutions. The answers were: Technical services of animal health and veterinary public health: 0%, 33,33%, 77,78%, 100%, 33,33%, 44,44%, 77,78%, 100% answered yes; 100%, 66,67%, 22,22%, 0%, 66,67%, 55,56%, 22,22%, 0% answered no. Health services: 22,22%, 66,67%, 88,89%, 44,44%, 55,56%, 22,22%, 0% , 77,78% answered yes; 77,78%, 33,33%, 11,11%, 55,56%, 44,44%, 77,78%, 100%, 22,22% answered no. Regional development planning services, 33,33%, 88,89%, 66,67%, 11,11%, 33,33%, 33,33%, 33,33%, 100% answered yes; 66,67%, 11,11%, 33,33%, 88,89%, 66,67%, 66,67%, 66,67%, 0% answered no; Regional staffing services 88,89%, 77,78%, 55,56%, 0%, 11,11%, 11,11%, 100%, 66,67% answered yes; 11,11%, 22,22%, 44,44%, 100%, 88,89%, 88,89%, 0%, !00%, answered no. In conclusion, based on the quantitative data collected, the Regional Organizationsâ€™ (OPD) assessment of the implementation of Veterinary Authority (Otovet) in rabies management policies were as follows:Â 4 OPDs scored well and 5 OPDs scored poor. Health services: 4 OPDs scored well and 5 OPDs scored poor. Regional development planning services: 4 OPDs scored well and 5 OPDs scored poor. Regional staffing services: 1 scored well and 8 scored poor.

Detection of Mycobacterium avium subspecies paratuberculosis Formula Milk in Bogor Widagdo Sri Nugroho; Minarwati Sudarwanto; Denny Widaya Lukman; Ewald Usleber
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 1 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.407 KB)

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which is usually associated to CrohnÃ¢â‚¬â„¢s disease (CD) in human. The disease shows similarities in clinical signs and pathology characteristic with JohnÃ¢â‚¬â„¢s disease (JD) in ruminants which is infected by MAP. Researchers in Europe, USA, and Australia detected MAP in their dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover the national demand. This situation keeps MAP as potential-problem in national dairy herd and human health in the future. The aim of this study was to detect MAP in the formula milk for toddler. Fifty samples from five established milk producers were taken on August 2006 at the supermarket in Bogor. Two separate diagnostic methods were used parallel in this study i.e polymerase chain reaction method (PCR) with insertion sequence F 57 as the primer and the Mycobacterial Growth Indicator Tube (MGIT). Neither MAP grew in MGIT after 20 weeks of incubation period but 5 samples were found positive by nested PCR. Although there was no evidence weather MAP grew from the samples, as well as in human to provide data on MAP in Indonesia. Key words: Mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR F57

Kadar Malondialdehid Tikus Diabetes Melitus Tipe 2 dengan Terapi Ekstrak Media Penumbuh Sel Punca Mesenkimal Ida Fitriana; Agustina Dwi Wijayanti; Puspa Wikan Sari; Raden Gagak Donny Satria; Dwi Cahyo Budi Setiawan; Yuda Heru Fibrianto; Widagdo Sri Nugroho
Acta VETERINARIA Indonesiana Vol. 5 No. 1 (2017): Januari 2017
Publisher : IPB University

Berbagai penelitian menunjukkan bahwa ekstrak media penumbuh sel punca mesenkimal (EMPSPM), tanpa sel punca itu sendiri, telah ditemukan terdapat berbagai faktor tropik hasil sekresi sel punca mesenkimal di dalam media kultur yang dapat meregenerasi jaringan yang rusak. Penelitian ini bertujuan untuk mengetahui gambaran kadar malondialdehid (MDA) dalam plasma dan ginjal tikus diabetes melitus tipe 2 (DMT2) dengan terapi EMPSPM. Dua puluh lima ekor tikus wistar digunakan dalam penelitian ini dibagi menjadi 5 kelompok yaitu kelompok DMT2 + 0,05 ml/kg BB EMPSPM (0,05); kelompok DMT2 + 0,1 ml/kg BB EMPSPM (0,1); kelompok DMT2 + 0,2 ml/kg BB EMPSPM (0,2), kelompok kontrol DMT2 (DMT2), dan kelompok sehat (KS). Induksi DMT2 dengan menggunakan streptozotosin nikotinamid (STZ-NA). Terapi mulai dilakukan pada hari ke 7 setelah kondisi DM tercapai, diberikan 4 kali dengan selang waktu 7 hari secara intraperitoneal. Data kadar glukosa darah dan MDA dianalisi secara statistik. Hasil penelitian menunjukkan bahwa induksi DMT2 dengan STZ-NA dapat menaikkan kadar glukosa dan MDA dalam darah (P<0,05). Terapi 0,05; 0,1; dan 0,2 ml/kg BB EMPSPM menunjukkan dapat menurunkan kadar glukosa darah, kadar MDA plasma dan ginjal (P<0,05). Kadar 0,2 ml/kg BB EMPSPM menunjukkan aktifitas lebih baik dalam menurunkan kadar glukosa darah dan MDA. Berdasarkan penelitian ini, EMPSPM dapat menurunkan kadar glukosa darah serta kadar MDA dalam darah dan ginjal tikus DMT2.

Isolasi dan Sensitifitas Antibiotika terhadap Streptococcus spp dari Kambing PE Mastitis Subklinis Kronis Widodo Suwito; AETH Wahyuni; Widagdo Sri Nugroho; Bambang Sumiarto
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Penelitian ini dilakukan untuk isolasi dan sensitifitas antibiotika terhadap Streptococcus spp dari susu kambing PE mastitis subklinis kronis di Kabupaten Sleman, Yogyakarta. Sebanyak 10 sampel susu kambing PE mastitis subklinis kronis dari Kabupaten Sleman digunakan dalam penelitian ini. Kriteria kambing PE mastitis subklinis kronis dengan uji California Mastitis Test (CMT) dua kali yaitu saat laktasi dan laktasi berikutnya dengan hasil positif (++) atau (+++) yang diikuti dengan penghitungan jumlah sel somatik (JSS) dengan metode Breed. Isolasi Streptococcus spp., dengan kultur dalam media blood agar selanjutnya di inkubasikan pada suhu 37°C selama 24 jam. Sensitifitas antibiotika terhadap Streptococcus spp., dengan agar difusi menggunakan kertas cakram antibiotika yang sudah diketahui konsentrasinya. Saat uji CMT pertama terdapat dua sampel positif (++) dan delapan sampel positif (+++) dengan rataan JSS masing-masing 2.400.000 sel/ml dan 4.475.000 sel/ml, sedangkan uji CMT kedua terdapat empat sampel positif (++) dan enam sampel positif (+++) dengan rataan JSS masing-masing 2.775.000 sel/ml dan 4.550.000 sel/ml. Susu kambing PE mastitis subklinis kronis dari Kabupaten Sleman dapat diisolasi Streptococcus spp., sebanyak 8 isolat dengan tipe hemolitik α, β, dan γ masing-masing 2, 1, dan 5 isolat. Streptococcus spp., resisten terhadap penisilin sedangkan terhadap tetrasiklin, ampisilin, eritromisin, dan sulfonamide masih sensitif. Penelitian ini menunjukkan bahwa Kambing PE mastitis subklinis kronis dari Kabupaten Sleman disebabkan oleh Streptococcus spp., dengan penisilin resisten, sedangkan tetrasiklin, ampisilin, eritromisin, dan sulfonamide masih sensitif.

Detection of Antibiotic Residues in Chicken Meat and Eggs from Traditional Markets at Yogyakarta City Using Bioassay Method Dyah Ayu Widiasih; Yatri Drastini; Doddi Yudhabuntara; F. Lintang R. Daru Maya; Prisha Lini Sivalingham; Heru Susetya; Widagdo Sri Nugroho Sri Nugroho; M. Th. Khrisdiana Putri; Roza Azizah Primatika; Bambang Sumiarto
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).

PCS-11 Mesenchymal Stem Cell-Conditioned Medium Improve the Recovery of Pancreatic α and β Cells in Type 1 Diabetes Mellitus Dian Bekti Hadi Masithoh; Yuda Heru Fibrianto; Marla Anggita; Widagdo Sri Nugroho; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.516 KB)

Mesenchymal stem-cell is a pluripotent cells that can be differentiated into many kinds of cells (1) and have abilities to differentiated into mature cell, secreted cytokines, and releasing many kinds of protein functioned in body body regulation (2). However, the therapeutic effect of stem cell arise from their secreted factor such as growth factors, cytokines, chemokines, and metabolits which acts as biologic regulator in the autocrine and paracrine body function (3). Secreted factors of mesenchymal stem cells known as secretome or mesenchimal stem cell conditioned medium (MSC-CM) have therapeutic effect for antiapoptosis, angiogenic, immunomodulatoric, and chemoattraactive activity (4). Mesenchymal stem cell conditioned medium used as the replacement agent for cell therapy because it contains no cells and save for used in different individual (5).Type 1 diabetes mellitus (DMT1) is a disorder characterized by destruction of the insulin-producing pancreatic β-cell that progressively leads to insulin deﬁciency and resultant hyperglycemia. Insulin deﬁciency can lead to progressive metabolic derangement, with worsening hyperglycemia, ketoacidosis, starvation, and death (6). Glucagon is a hormone produced by pancreatic α cells. Glucagon releases by the pancreas to raise the concentration of glucose in the bloodstream when the concentration of glucose in the bloodstream too low (7). Glucagon worked in opposite with insulin, which lowers the extracellular glucose level, while insulin works to increase the extracellular glucose level (8). The aim of this study was to investigate the role of MSC-CM on the structural and functional regeneration of pancreatic α and β cells in Wistar rat (Rattus norvegicus) induced with type 1 diabetes mellitus. The datas in this study will completes the information about the effect of MSC-CM on pancreatic cells regeneration, which has been presented in previous study (9).

Co-Authors Abdulwahed Ahmed Hassan Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo

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