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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Ilmiah Medicamento Jurnal Pengabdian Kepada Masyarakat Itekes Bali
I Gusti Bagus Teguh Ananta
Institut Teknologi Dan Kesehatan Bali
Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Health Professions Medicine & Pharmacology Nursing Public Health

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AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG (Musa sp.) TERHADAP Escherichiacoli DAN Staphylococcus aureus SERTA IDENTIFIKASI GOLONGAN SENYAWA AKTIFNYA Ida Ayu Raka Astiti Asih; Wiwik Susanah Rita; I Gusti Bagus Teguh Ananta; Ni Kadek Dyan Mustika Sri Wahyuni
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 1 (2018): Volume 6, Nomor 1, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.665 KB)

Abstract

ABSTRAK: Kulit pisang (Musa sp.) dapat digunakan sebagai bahan penghambat bakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kulit pisang (Musa sp.) terhadap Escherichia coli dan Staphylococcus aureus serta identifikasi senyawa aktifnya.. Ekstraksi dilakukan dengan teknik maserasi dengan etanol, selanjutnya ekstrak etanol dipartisi berturut-turut dengan n-heksana, etilasetat, dan n-butanol. Ekstrak n-heksana, etil asetat, dan n-butanol, yang diperoleh diuji fitokimia dan aktivitas penghambatan terhadap bakteri Escherechia coli dan Staphylococcus aureus. Uji penghambatan bakteri dilakukan dengan metode sumur difusi pada media Nutrient Agar (NA).Ekstraksi 500 g kulit pisang (Emas, Kepok, Susu dan Kayu) masing-masing menghasilkan 15, 20, 10, 12 gram ekstrak pekat yang berwarna kuning. Hasil uji fitokimia menunjukkan bahwa ekstrak kulit pisang mengandung senyawa alkaloid, terpenoid, fenol dan flavonoid. Hasilujiaktivitas terhadap bakteri Escherechia coli dan Staphylococcus aureus menunjukkan bahwa ekstrak kulit pisang Mas dan Kepok adalah ekstrak yang aktif dengan MIC masing masing 1% dan 0,5%, untuk kulit pisang mas dan 0,5% serta 0,1%, untuk kulit pisang kepok. Kata Kunci: Antibakteri, Musa sp., Escherichia coli, Staphylococcus aureus, Minimum Inhibitory Concentration (MIC) ABSTRACT: The banana peel (Musa sp.) can be used as antibacteria. This study aims to determine the antibacterial activity of banana peel (Musa sp.) against Escherichia coli and Staphylococcus aureus and to identify their active compounds. Extraction was done by maceration technique with ethanol, then ethanol extract was partitioned successively with n-hexane, ethylacetate, and n –butanol to get n-hexane, ethyl acetate, and n-butanol extract. The extract was tested phytochemical and activity of against Escherichia coli and Staphylococcus aureus. The antibacterial activity test was performed by diffusion well method on Nutrient Agar (NA) medium. Extraction of 500 g banana peel (Mas, Kepok, Susu and Kayu) each produced 15, 20, 10, 12 grams of yellow-colored concentrated extract. The phytochemical test results showed that banana peel extract contains alkaloids, terpenoids, phenols and flavonoids. The antibacterial activity test of the banana peels against Escherechia coli and Staphylococcus aureus showed that banana extract of Mas and Kepok was the active extract with MIC 1% and 0.5%, for mas and 0.5% and 0,1%, for kepok peel.
POTENSI EKSTRAK LIMBAH KULIT PISANG LOKAL (Musa sp) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus Aureus I Gusti Bagus Teguh Ananta; Wiwik Susanah Rita; I Made Oka Adi Parwata
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 1 (2018): Volume 6, Nomor 1, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Penelitian ini dilakukan unuk menentukan potensi ekstrak limbah kulit pisang lokal sebagai antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Penelitian yang dilakukan meliputi penentuan ekstrak limbah kulit pisang lokal (pisang mas, susu dan kayu) yang terbaik dalam menghambat pertumbuhan bakteri, penentuan pelarut terbaik, dan konsentrasi hambat minimum. Hasil penelitian menunjukkan bahwa ekstrak metanol merupakan ekstrak yang paling aktif dalam menghambat pertumbuhan bakteri dengan daya hambat sebesar 19,17 mm untuk bakteri Escherichia coli dan 18,67 mm untuk bakteri Staphylococcus aureus pada konsentrasi 10 %. Ekstrak n-butanol limbah kulit pisang mas memberikan daya hambat bakteri yang lebih baik dibandingkan ekstrak air, n-heksana, dan etil asetat yaitu sebesar 17,15 mm untuk bakteri Escherichia coli dan 16,37 mm untuk bakteri Staphylococcus aureus. Konsentrasi hambat minimum ekstrak n-butanol menunjukkan hasil hambatan pertumbuhan bakteri Escherichia coli adalah sebesar 0,5 % dengan daya hambat sebesar 7,65 mm dan 0,5% untuk bakteri Staphylococcus aureus dengan daya hambat sebesar 5,55 mm. Uji fitokimia menunjukkan bahwa senyawa yang berperan dalam aktivitasnya sebagai antibakteri yaitu alkaloid, terpenoid, flavonoid, fenol dan saponin. Kadar total fenol yang terdapat dalam ekstrak n-butanol limbah kulit pisang mas adalah sebesar 250,17 mg GAE/ 100 g dan kadar total flavonoid sebesar 129,07 mg QE/100g Kata Kunci : Antibakteri, Escherichia coli, Staphylococcus, Musa Paradisiaca, ABSTRACT: This research has been conducted to determine the potency of banana local peel waste (Musa sp) as antibacteria towards Escherichia coli and Staphylococcus aureus. The research included determining of the best extract of local banana peel waste (banana mas, susu, and kayu) to inhibit bacterial growth, the best solvent, and minimum inhibitory concentration. The results showed that the methanol extract has the best one to inhibit the growth of both bacteria E. coli and S. aureus with the inhibition zone of 19.17 mm and 18.67 mm respectively at concentration 10%. n-butanol extract of banana mas (Musa accuminata, AA) peel waste was the best one to inhibit both bacteria compared to water, n-hexane, and ethyl acetate extract with the inhibition zone of 17.15 mm for Escherichia coli and 16.37 mm for Staphylococcus aureus. The minimum inhibitory concentration of n-butanol extract towards both E. coli and S. aureus was 0.5% with the inhibitory zone of 7.65 mm and 5.55 mm respectively. Phytochemical screening result indicated the presence of alkaloid, terpenoid, phenols, flavonoids, and saponins which may influence the antibacterial activities. Total phenols content of the extract was 250.17 mg GAE/ 100 g while total flavonoids content was 129.07 mg QE/ 100 g.
Potensi Ekstrak Buah Cabai Merah Keriting (Capsicum annum var. Longum) sebagai Antioksidan dan Antibakteri I Gusti Bagus Teguh Ananta; Dewa Gede Anom Anjasmara
Jurnal Ilmiah Medicamento Vol 8 No 1 (2022): Jurnal Ilmiah Medicamento
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v8i1.3170

Abstract

Chili (Capsicum annum var. Longum) is a horticultural plant that is widely used as raw material for the food and pharmaceutical industry. Generally, chili fruit contains phenolic chemical compounds of flavonoid and capcaisinoid compounds. Bioactive compounds in chili such as phenols, flavonoids and capsaicinoid have a positive relationship to antioxidant and antimicrobial activity. Capsicum annum var. Longum contains secondary metabolites such as alkaloids, phenols, flavonoids, tannins and saponins. The total phenol content is 0.81% (807.76 mg GAE/100 g). The total content of flavonoids is 5.64% (5646.08 mg QE/100 g). Antioxidant activity was carried out using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with an IC50 value of 505.35 ppm. Antibacterial activity was carried out by disc diffusion method with the growth inhibition of Escherichia coli bacteria of 8.56 mm and Staphylococcus aureus of 3.55 mm.
Counseling on the Function of Vitamins in Increasing Body Endurance and Training on Hand Washing Movements, Wearing Masks, and Keeping Distance to the Marching Band Genta Bhuwana Ganeswara Ananta, I Gusti Bagus Teguh; Anjasmara, Dewa Gede Anom; Indrayoni, Putu
Jurnal Abdimas ITEKES Vol 1 No 1 (2021): Jurnal Abdimas ITEKES Bali September
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Institute Teknologi dan Kesehatan (ITEKES) Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37294/jai.v1i1.396

Abstract

Various preventive measures in tackling the increase in COVID-19 cases must be carried out, both by the government and the community. Preventive efforts are so far the best practice to reduce the impact of the COVID-19 pandemic, given that there is no treatment that is considered effective against the SARS-CoV-2 virus. One of the government's efforts to overcome the impact of the COVID-19 pandemic is by implementing the 3M movement, namely washing hands, wearing masks, and maintaining distance. Marching band is one of the art and sports learning activities that have been the impacted by the COVID-19 pandemic. Music learning methods in the classroom can be done online, but in practice mastery of musical instruments needs to be done offline. The offline training process needs to get more attention by implementing the 3M protocol, namely maintaining distance, using masks, and washing hands. Based on this, counseling activities on the function of vitamins were carried out in increasing endurance as well as training in washing hands, wearing masks, and keeping a distance. The implementation was carried out by conducting counseling and training which was attended by 25 participants. The results of the activity showed that there was an increase in the knowledge of the training participants regarding the COVID-19 pandemic and the movement to wash hands, wear masks and keep a distance, >80% of Marching Band members Genta Bhuwana Ganeswara had good knowledge.
Penyuluhan Pembuatan Sirup Berbahan Dasar Cascara “Limbah Kopi” widnyani, Ida Ayu Putu Ary; Yoga, Wahyu Krisna; Sintyadewi, Putu Rima; Bestari, Ida Ayu Purnama; Padmiswari, A.A Istri Mas; Ananta, I Gusti Bagus Teguh
Jurnal Abdimas ITEKES Vol 4 No 1 (2024)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Institute Teknologi dan Kesehatan (ITEKES) Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37294/jai.v4i1.634

Abstract

Meningkatnya tingkat konsumsi kopi mempengaruhi produktivitas industri pengolahan kopi. Cascara merupkan limbah pada proses pengolahan kopi, yaitu bagian kulit kopi yang sudah mengalami proses pengeringan. Tujuan dari kegiatan ini adalah memberikan penyuluhan mengenai pengolahan limbah kopi (Cascara) menjadi produk sirup yang memiliki nilai ekonomi lebih tinggi kepada siswa siswi di SMK Negeri 1 Kintamani. Kegiatan diawali dengan pengukuran tingkat pengetahuan dengan pemberian pretest, selanjutnya diberikan materi berupa penyuluhan pemanfaatan, senyawa bioaktif, dan pengolahan Cascara. Proses evaluasi keberhasilan kegiatan dilakukan dengan pemberian post test dan kuesioner kepuasan peserta kegiatan. Data yang didapatkan kemudian diolah dan disajikan dalam bentuk tabel presentase. Hasil pretest yang didapatkan sebanyak 20,59% memiliki tingkat pengetahuan baik, 29,41% memiliki tingkat pengetahuan cukup, dan 50% masaih dalam kategori kurang. Setelah diberi penyuluhan hasil dari post test peserta menjadi 79,41% dalam kategori baik dan 20,59% cukup. Hasil kuesioner kepuasan peserta menunjukkan 38,23% peserta menjawab setuju dan 61,77% menjawab sangat setuju bahwa kegiatan penyuluhan berjalan dengan lancar, menarik, dan bermanfaat Kata kunci : Cascara, limbah kopi, pengolahan, sirup
Co-Authors Anjasmara, Dewa Gede Anom Dewa Gede Anom Anjasmara I Made Oka Adi Parwata I. A. R. Astiti Asih Ida Ayu Purnama Bestari Ni Kadek Dyan Mustika Sri Wahyuni Padmiswari, A.A Istri Mas Putu Indrayoni Putu Rima Sintyadewi Widnyani, Ida Ayu Putu Ary WIWIK SUSANAH RITA Yoga, Wahyu Krisna