Garuda - Garba Rujukan Digital

Antioxidant Activity of Methanol Extract from Several Indonesian Green Teas simamora, adelina; Steven, Michael; Santoso, Adit Widodo; Rumiati, Flora; Timotius, Kris Herawan
Jurnal Kedokteran YARSI Vol 26, No 1 (2018): JANUARI - APRIL 2018
Publisher : Lembaga Penelitian Universitas YARSI

As a common beverage, green tea is supposed to have beneficial health effect, such as antioxidant. At present, there are many green teas available in the market in Jakarta. Their quality, in their antioxidant activity, should be proved. This study aimed to investigate the antioxidant activity of extracts of green tea products commercially available in Jakarta. Four green tea samples from different factories (coded as A, B, C, and D) were selected and macerated using methanol. DPPH radical scavenging activity, reducing power ability and total antioxidant capacity were used to measure the antioxidant activity. The total phenolic content (TPC) was also determined. The studied green teas had varied TPC from 23.80 to 84.03 mG GAE/g extract, ranked as B > D > A > C. All samples exhibited various but strong antioxidant activity by DPPH assay, even better than standards ascorbic acid and butylated hydroxytoluene (BHT). However, all extracts showed similar activities in their reducing power ability and total antioxidant capacity, with activities less than standards. These findings confirm that the quality of the Indonesian commercial green teas were heterogeneous both in TPC or DPPH scavenging capacity. But, there is an indication that they are good as an antioxidant containing beverage.

Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt Widodo Santoso, Adit; Simamora, Adelina; Simamora, Adelina; Herawan Timotius, Kris; Herawan Timotius, Kris
Jurnal Kedokteran YARSI Vol 26, No 2 (2018): MEI - AGUSTUS 2018
Publisher : Lembaga Penelitian Universitas YARSI

Myristica fragrans Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from M. Fragrans. The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from M. fragrans mace. Both WE and EO were evaluated for their α-glucosidase inhibitory activities in vitro and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC50 = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC50 = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm. The strongest activity was observed against Staphylococcus mutans. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities.

Bioactivities of Water Extract and Essential Oil from the Mace of Myristica fragrans Houtt Widodo Santoso, Adit; Simamora, Adelina; Simamora, Adelina; Herawan Timotius, Kris; Herawan Timotius, Kris
Jurnal Kedokteran YARSI Vol 26, No 2 (2018): MEI - AGUSTUS 2018
Publisher : Lembaga Penelitian Universitas YARSI

Myristica fragrans Houtt (nutmeg) is used as a spice and flavour for food and beverages. It has been traditionally used to treat a number of medical conditions, including diabetes mellitus. The study was undertaken to scientifically validate the traditional use of mace from M. Fragrans. The objectives of this study were to evaluate α-glucosidase inhibition, antioxidant and antibacterial activities of water extract (WE) and essential oil (EO) from M. fragrans mace. Both WE and EO were evaluated for their α-glucosidase inhibitory activities in vitro and their antioxidant activities based on DPPH radical scavenging assay. Standard compounds were used for every test. Total phenolic and flavonoid contents of both extracts were also determined. The extracts were also tested for their antibacterial activities against six different bacteria by a well diffusion method. Both extracts showed inhibition activities against α-glucosidase, with WE showed stronger activity than EO (IC50 = 1.86 and 8.15 mg/ml). Good radical scavenging activities were observed for both extracts, with WE showed stronger activity than EO (IC50 = 1.51 and 4.59 mg/ml). WE showed higher content in phenolic than EO (47.84 and 37.21 mg GAE/100 g DW). Flavonoid content in WE was also higher than EO (215.36 and 30.12 mg RE/ml). Based on the well diffusion method, only EO exhibited antibacterial activities, with inhibition zone in the range 1.03 – 1.30 mm. The strongest activity was observed against Staphylococcus mutans. The results indicate WE and EO can be exploited further for pharmacological uses, in particular for their antidiabetic and antioxidant activities.

Identifikasi Telur Cacing Usus dan Kista Protozoa Usus pada Tubuh Lalat dari Warung Makan di Tanjung Duren Timur Jakarta Barat Caecilia A. P. Wulandari; Esther S. Majawati; Adelina Simamora
Majalah Kedokteran UKI Vol. 35 No. 1 (2019): JANUARI - MARET
Publisher : Fakultas Kedokteran Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/mkvol34iss2pp60

Abstrak Penularan penyakit pada manusia dapat terjadi melalui banyak cara antara lain melalui lalat sebagai vektor mekanik. Lalat dapat membawa bakteri patogen, protozoa, larva serta telur cacing yang menempel pada tubuhnya dan dapat mencemari bahan makanan. Tujuan penelitian ini untuk mengidentifikasi telur cacing usus dan protozoa usus pada tubuh lalat di warung makan kelurahan Tanjung Duren Timur, Jakarta Barat. Desain penelitian yang digunakan dalam penelitian ini adalah studi deskriptif dengan pendekatan poton lintang. Penelitian dilakukan di Tanjung Duren Timur, Jakarta Barat. Hasilnya, 38 warung tempat penangkapan lalat seluruhnya positif mengandung parasit. Parasit yang ditemukan adalah telur cacing tambang, Ascaris lumbricoides dan kista Entamoeba histolytica pada 17 (44,8%) warung makan, kista Giardia lamblia pada 12 (31,6%) warung makan, dan kista Entamoeba coli pada 32 (84,2%) warung makan. Kata kunci: telur cacing usus, protozoa usus, vektor mekanik, lalat. Abstract Transmission of diseases can occur in many ways, including through flies as mechanical vectors. Flies can carry pathogenic bacteria, protozoa, larvae and worm eggs that attached to their bodies and can contaminate food. The purpose of this cross-sectional study was to identify eggs of the intestinal worm and intestinal protozoa attached to the body of flies at food stalls in Tanjung Duren Timur, West Jakarta. As a result, all (n=38) stalls where flies were caught were entirely positive for parasites. Hookworm eggs, Ascaris lumbricoides and Entamoeba histolytica cysts were found in 17 (44.8%) food stalls; Giardia lamblia cysts in 12 (31.6%); and Entamoeba coli cysts in 32 (84.2%).Keywords: eggs of intestinal worms, intestinal protozoa, mechanical vectors, flies.

Identifikasi Telur Cacing pada Kubis (Brassica oleracea) pada Pasar Swalayan Jessica Vanesa Yahyadi; Esther Sri Majawati; Adelina Simamora
Jurnal Kedokteran Meditek VOL. 23 NO. 62 APRIL-JUNI 2017
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v23i62.1550

Penyakit infeksi yang berhubungan dengan soil transmitted helminth masih merupakan masalah kesehatan di Indonesia. Umumnya cacing berasal dari golongan Ascaris lumbricoides, Trichuris trichiura, Necator americanus, Ancylostoma duodenale. Penyakit ini berhubungan erat dengan keadaan sosial ekonomi, kebersihan diri dan lingkungan. Sayuran mentah dapat menjadi salah satu agen transmisi telur cacing. Mengonsumsi sayuran mentah dapat meningkatkan kejadian infeksi parasit. Penelitian ini bertujuan untuk mengidentifikasi ada tidaknya kontaminasi telur soil transmitted helminth pada sayuran kubis yang di jual di pasar swalayan di Jakarta. Penelitian ini bersifat deskriptif, dilakukan pada bulan Oktober hingga November 2016. Sampel diperoleh secara consequtive dari 62 pasar swalayan di sekitar Jakarta. Pemeriksaan telur cacing dilakukan secara mikroskopis di Laboratorium Parasitologi, Fakultas Kedokteran Universitas Kristen Krida Wacana. Dari hasil penelitian ditemukan soil transmitted helminth positif pada satu sampel dari 62 (1,61%) sayuran kubis yang dijual di pasar swalayan sekitar Jakarta. Sayuran terkontaminasi oleh telur Ascaris lumbricoides, sedangkan Trichuris trichiura, Necator americanus dan Ancylostoma duodenale tidak ditemukan.Kata kunci : Soil Transmitted Helminth, Kubis, Ascaris lumbricoides, pasar swalayan

Antioxidant, Antidiabetic, and Anti-obesity Potential of Ipomoea reptans Poir Leaves Hendrik Kurniawan; Ermenilda Sonia Dacamis; Adelina Simamora; Priscilla Sari Dianauli Lumban Tobing; Ali Hanapiah; Adit Widodo Santoso
Borneo Journal of Pharmacy Vol. 3 No. 4 (2020): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v3i4.1583

Ipomoea reptans Poir or kangkung is a popular leafy vegetable, a favorite to people in Asian countries. However, limited information is available on their bioactivities. In the present study, the antioxidant, antidiabetic, and anti-obesity potential of I. reptans leaves were investigated. Different fractions (ethanol, ethyl acetate, and hexane) of I. reptans leaves were evaluated for their scavenging activity on DPPH radicals, whereas their reducing potential was investigated by cupric reducing antioxidant capacity (CuPRAC), total antioxidant, and reducing power assays. The antidiabetic potential was investigated by their inhibition effect on α-glucosidase. Total phenolic and flavonoid contents of I. reptans leaves were solvent dependent. Ethyl acetate contained the highest phenolic content, followed by ethanol and hexane fractions. However, for flavonoid content, the order was ethanol > ethyl acetate > hexane. All fractions showed DPPH scavenging activity in a concentration-dependent manner, with activities weaker than standards ascorbic acid and BHT, in the order of ethanol > ethyl acetate > hexane. All fractions showed reducing capacity, but only hexane and ethanol fractions of I. reptans leaves showed inhibition on α-glucosidase, with hexane showed more potent inhibition compared to acarbose. The study also found that fractions of I. reptans inhibit lipase and trypsin, enzymes related to lipid metabolism. Findings in this study offer a prospect for I. reptans leaves as a functional food source for antioxidant, antidiabetic, and anti-obesity purposes.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search