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All Journal Jurnal Labora Medika
Nasruddin Nasruddin
Magister Ilmu Laboratorium Klinik, Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology

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GAMBARAN HISTOLOGI FASE REMODELLING JARINGAN LUKA KRONIK KULIT MENCIT SETELAH PEMBERIAN PERLAKUAN PLASMA JET Gela Setya Ayu Putri; Ali Ali; Nasruddin Nasruddin
Jurnal Labora Medika Vol 6, No 1 (2022): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.348 KB) | DOI: 10.26714/jlabmed.6.1.2022.1-6

Abstract

The medical aspect of plasma conceptually is concerned with the ability of plasma to produce biological molecules, such as RONS, which can be physically controlled through dose and behavior. RONS in micro concentrations was reported to have wound healing properties. The effect of a plasma jet on chronic skin wounds of mice on day 14 which theoretically included the remodeling phase has not been widely studied. The aim of the study was to calculate the number of inflammatory cells, fibroblasts, blood vessels, the percentage of re-epithelization, and the percentage of necrosis. Experimental research method with an analytic descriptive design. The results of microscopic observations showed that based on the number of inflammatory cells (round cells) data, the control group was significantly higher than the non-contact plasma (P<0.05). Based on the blood vessel count data, it was found that the control group was significantly higher (P<0.05) than the contact group, non-contact group and non-contact group. Based on the data on the number of fibroblasts, the results in contact-non-contact group were significantly (P<0, 05) higher than control group and plasma-contact group. Based on the data on the percentage of reepithelialization, the results were different in all treatment groups but not significant (P>0.05). Based on the data on  the percentage of necrosis, the results were were different in all treatment groups but not significant (P>0.05).
Gambaran Mikroskopis Jaringan Kulit Normal Mencit Balb/C Setelah Perlakuan Plasma Jet Dengan Pengecatan Hematoxylin Eosin Nia Salsabila; Nasruddin Nasruddin; Tulus Ariyadi; Gela Setya Ayu Putri
Jurnal Labora Medika Vol 5, No 2 (2021): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.665 KB) | DOI: 10.26714/jlabmed.5.2.2021.57-62

Abstract

Terdapat banyak pilihan pengobatan untuk perbaikan penyembuhan luka. Eksplorasi metode seperti perlakuan terhadap agen teknologi pengobatan plasma untuk terapi medis menghasilkan wawasan baru. Plasma merupakan zat keempat setelah padat, cair dan gas yang mampu menghasilkan Reactive Oxygen and Nitrogen Species (RONS) yang bermanfaat bagi kesehatan jika dosisnya tepat. Penelitian ini bertujuan untuk menguji pengaruh plasma jet pada kulit normal mencit yang rusak akibat pengaruh dosis berlebihan secara histologis dengan pewarnaan hematoxylin eosin (HE). Penelitian menggunakan mencit Balb/c 4 bulan yang dibagi menjadi 3 kelompok jarak paparan. Jarak yang paling berpengaruh pada kulit diperiksa secara mikroskopis untuk mengetahui perbedaan respon inflamasi pada 2 pengamatan yaitu jaringan yang rusak pada kulit dibedah pada hari ke-0 dan hari ke-1. Pada hari ke-0, jaringan kulit mencit dibedah 4 jam setelah terpapar plasma jet (D0) dan hari 2 tikus dibedah 24 jam setelah terkena plasma jet (D1). Hasil penelitian secara visual menyebabkan kerusakan kulit dalam jarak 3 mm. Hasil pengamatan kerusakan mikroskopis menunjukkan bahwa Paired T-test dengan nilai probabilitas 0,238 (P>0,05) berarti kedalaman kerusakan kulit D0 dan D1 tidak berbeda nyata. Kesimpulannya, jarak pancaran plasma 3 mm berpotensi merusak kulit normal, secara mikroskopis kerusakan kulit hanya terjadi pada epidermis, dan kedalaman kerusakan kulit tidak signifikan antara kulit yang dioperasi pada hari ke-0 dan hari-1.
Co-Authors Ali Ali Gela Setya Ayu Putri Nia Salsabila Tulus Ariyadi