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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI

Polmer Situmorang

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Author-ID : 383053

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Ovulation rates and twinning birth following Follicle Stimulating Hormone (FSH) treatment at differents stages of estrus cycle Polmer Situmorang; D.A Kusumaningrum; R Sianturi
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Twinning rate could be increased through genetically approach or through hormonal induction approach. The aim of this study was to observe the effect of first injection of FSH initiated on the ovulation rate. The experiment design was completely randomized with 3 different time of FSH was initiated as treatment using 12 lactating dairy cattle. Injection of FSH was initiated at day 2 (Treatment I), day 10 (Treatment II) and at day 18 (Treatment III) of estrus cycle. A total of 6 ml Folltropin (Equivalent 120 mg FSH) was intramussculary injected in decreasing dossis method twice a day with 12 hours interval for 4 days. Blood was collected on day 12 of estrus cycle for progesterone level. Data recorded were the diameter of ovary (DO), total corpus luteum (TCL), concentration of progesterone (P), percentage of pregnancy and number of birth. The means DO, TCL and P were significantly (P < 0.05) higher after injection (2.0 cm; 2.1 and 1.6 ng/ml) than those before injection (1.4 cm, 1.0 dan 0.6 ng/ml). The time of first injection of FSH was initiated, significantly affect the ovulation rate. The mean DO, TCL and P were significantly higher (P < 0.01) in treatment II (2.6 cm; 4.0 and 2.9 ng/ml) than those in treatments I (1.9 cm; 1.3 and 0.9 ng/ml) or in treatment III (1.6 cm; 1.3 and 0.9 ng/ml). There was no significant diference between treatment I and III. The percentage of pregnant were 25.0; 75.0 and 25.0 for treatments I; II and III respectively. One twin birth and 1 single birth were obtained in treatments II but only one single birth for each treatment I and III. Number of CL were positively correlated with the concentration of progesterone but were not fully useful for prediction number of birth. In conclusion, the dairy cattle gave a better response to exogenous gonadotropin hormone when the first injection was initiated at day 10 of estrus cycle. Key Words: Hormone, FSH, Estrus Cycle, Ovulation, Twin Birth

Effects of the addition of hormone in maturation medium for in vitro production ofembryo (IVP). Polmer Situmorang; Endang Triwulanningsih; Adriana Lubis; Nurhasanah Hidayati; Tatit Sugiarti
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 1 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study on the effects of hormone FSH, hCG and estrogen in maturation medium on embryo production (IVP) was conducted. Ovaries of dairy cows were obtained from slaughtered house and oocytes were collected by aspiration and slicing. Oocytes were matured in TCM-199 media containing one of each hormonal treatments : 10 ug/ml FSH, 2 IU hCG, 1 ug/ml estrogen, 10 ug/ml FSH + 2 IU hCG, 10 ug/ml FSH + 1 ug/ml estrogen, and 10 ug/ml FSH + 2 IU hCG + 1 ug/ml estrogen for 24 hours. Fertilization was conducted in thyroid albumin lactate pyruvate (TALP) media containing 10 ug/ml heparin for 18-24 hours and co-cultured in synthetic oviduct fluid (Sof-media) using C02 incubator at 38oC. Every 48 hours the embryos were moved into fresh Sofmedia and embryo evaluation was done on day 7using a microscope . From a total of 293 oocytes studied resulted 60.4% of zygotes which develop to 9.4% young embryos ( cell numbers <16), 45.3% morulae and 5,7% blastocysts. Hormones used singly did not significantly affect the production of embryo . The highest mean percentages of fertilization wasobtained in FSH (73.3%) and the lowest in estrogen (59.6%), but the mean percentage of blastocyst was higher in estrogen . The mean percentages of young embryos, morulae and blastocysts were 4.8, 66.1, 2.4 ; 18 .8, 41 .6, 6.6 and 3 .3, 46.6, 10 .0 for FSH, hCG and estrogen, respectively . Using a combination of FSH with hCG and estrogen did not significantly increase the production of embryo . The mean percentages of fertilization, young embryo, morulae and blastocysts were 55.2, 9.3, 43.4 and 2.6 ; 51 .7, 4.8, 35.8 and 11 .1 and 56 .0, 12 .3, 42.3 and 1 .4 for FSH+hCG, FSH+estrogen and FSH+hCG+estrogen respectively . Keywords: Embryo, fertilization, hormone, in vitro

The Prospects of Using Chilled Semen to Increase the Production of Dairy Cattle Polmer Situmorang
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 13, No 1 (2003): MARCH 2003
Publisher : Indonesian Center for Animal Research and Development

Dairy cattle production in Indonesia has been rapidly developed since the importation of live cattle from Australia and New Zealand in 1970. Technology of artificial insemination (AI) play an important role to increase the production of dairy cattle by using frozen semen from both importation or produced in Centre for Artificial Incemination Lembang and Singosari. Nevertheless, frozen semen is not without problems because a more than less 30% of sperm are killed during the freezing procedure and those who survive are sensitive to enviroment and has a low fertility. Therefor the using of chilled semen will be an alternative to be considered to replace frozen semen. Chilled semen can be simply produced and not required a complex laboratory and an expensive equipment. Other advantages is the production of straw per bull and fertility was higher than those frozen semen and resulting an increased of farmer income. Case study in Pangalengan West-Java showed a lower production cost of chilled semen than those cost for purchasing frozen semen. Widely aplication of the using of chilled semen will reduce a dependency on frozen semen and importation of bull, and at the end will increase the national income. Keywords: Dairy cattle, frozen semen, chilled semen

PENGARUH FORMALIN DAN pH TERHADAP DAYA HIDUP SPERMATOZOA SAPI YANG DISIMPAN PADA SUHU RUANGAN MAUPUN 5°C Polmer Situmorang; E Triwulanningsih; T Sugiarti; DA Kusumaningrum; RG Sianturi
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1063

The study was conducted in Research Institute for Animal Production (RIAP)-Ciawi. Semen was collected from 3 bulls twice a week by artificial vagina (AV), diluted in Tris buffer (control) and containing of 0.0625% formalin, reducing pH to 5.5 or their combination to temporally inhibit the motility of sperm.Diluted semen were store at ambient temperature for 0, 3 and 24 hours or at 5°C for 0, 3 and 7 days.Reactivation of motility was performed by dilution of formalin and bring the pH to 7.0. In ambient temperature,formalin and pH significantly (p<0.01) inhibit the motility of sperm, but these inhibition was reversible. The percentage of motility was significantly higher(p<0.01) in formalin than in pH 5.5 or control, following 24 hours of storage periode. The inhibition effect of combination between formalin and pH was non permanent. It is show that the inhibition effect of treatments was permanent for semen stored at 5°C.

Co-Authors A Lubis Abdul Rachman Siregar Adriana Lubis D. A Kusumaningrum D.A Kusumaningrum DA Kusumaningrum E Triwulaningsih E Triwulanningsih Endang Triwulanningsih Kusumaningrum, DA Mozes R Toelihere Muhammad Rizal Amin Nurhasanah Hidayati R Sianturi RG Sianturi Sianturi, RG T Sugiarti Tatit Sugiarti Tuty L Yusuf Tuty L Yusuf W Caroline

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