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Pertumbuhan Planlet Anggrek Dendrobium sp. pada Media Cacahan Kulit Kayu dan Larutan Rooting Starter Selama Aklimatisasi Nurromadon, Bilham; Ropalia, Ropalia; Zasari, Maera
JURNAL AGROTROPIKA Vol 23, No 2 (2024): Jurnal Agrotropika Vol 23 No 2, Oktober 2024
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v23i2.8656

Orchid plantlets are expected to be able to adapt and grow under optimal conditions during the acclimatization period. Selecting appropriate planting media and providing proper nutrition is one of the efforts that can be made to increase the success of high growth of Dendrobium, sp orchid plantlets during the acclimatization period. The aim of this research was to identify the appropriate planting medium and concentration of rooting starter solution to support the growth of Dendrobium sp orchid plantlets during acclimatization. This research was carried out from March to July 2022 at the Bangka Belitung University Experiment and Research Garden. This study used a factorial randomized block plan. The first factor is the planting medium; (M1) pine bark, (M2) Paraserianthes falcataria bark, (M3) acacia bark, and (M4) fern. The second factor is the industrial growth concentration 20 ml/l (K0) and 10 ml/l (K1). The results showed that the acclimatization of Dendrobium sp orchid plantlets was successful as seen from the large growth in plantlet height and the emergence of new shoots. The treatment that produced the highest growth of Dendrobium sp orchid plantlets during the acclimatization period was fern media and the best rooting starter concentration was 20 ml/l. The combination that showed the highest growth in Dendrobium sp. orchid plantlets during the acclimatization period was fern media + 20 ml/rooting starter. Key words: Dendrobium, tree bark, acacia bark, fern, rooting starter, Paraserianthes

Isolasi Bakteri Pendegradasi Keratin pada Pengomposan Limbah Bulu Ayam Yasfa, Maudy Alliya; Kusmiadi, Riwan; Ropalia, Ropalia
Rona Teknik Pertanian Vol 18, No 1 (2025): Volume No. 18, No. 1, April 2025
Publisher : Department of Agricultural Engineering, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/rtp.v18i1.40209

Abstrak.Bulu ayam merupakan limbah dari sisa indsutri rumah Pemotongan ayam (RPA). Pengolahan limbah bulu ayam (LBA) dapat dilakukan secara fisik (tekanan hidrolisis, penguapan, pembakaran), menggunakan bahan kimia (larutan asam kuat, larutan alkali). Metode tersebut tidak ramah lingkungan dan dapat menimbulkan polusi. Alternatif pengolahan LBA adalah penggunaan mikroorganisme yaitu bakteri pendengradasi dari kompos bulu ayam. Penelitian ini bertujuan untuk mendapatkan isolat bakteri yang berpotensi sebagai agen biodegradator keratin dalam kompos bulu ayam. Metode penelitian yang digunakan adalah metode eksplorasi dengan mengambil sampel hari ke-10 pengomposan dari campuran kotoran ayam (KA) dan bulu ayam (BA) serta sampel campuran kotoran sapi (KS) dan bulu ayam (BA) masing-masing perbandingan 1 : 1 (w/w). Isolasi bakteri dari limbah bulu ayam yang dikombinasikan dengan limbah ternak memiliki kelimpahan 1,4x109 - 16x109. Isolat yang didapatkan sebanyak 47 isolat yang menunjukkan aktivitas proteolitik yang terdiri dari 40 isolat berasal dari campuran KS dan BA serta 7 isolat berasal dari campuran KA dan BA. Dari 47 isolat yang memiliki indeks zona bening ideal sebanyak 11 isolat dengan persentase 23%. Indeks zona bening yang ideal berkisar dari 4,86 0,73 sampai 2,40 0,59.Isolation of Keratin Biodegradators from Chicken Feather Waste in Composting ProcessAbstract. Chicken feathers are waste from the chicken slaughterhouse (RPA) industry. Processing of chicken feather waste (LBA) can be done physically (pressure hydrolysis, evaporation, burning) or with chemicals (strong acid solutions, alkali solutions). This method is not environmentally friendly and can cause pollution. An alternative to LBA processing is using microorganisms, namely degrading bacteria from chicken feather compost. This research aims to obtain bacterial isolates that have the potential to act as keratin biodegrading agents in chicken feather compost. The research method used was an exploratory method by taking samples on the 10th day of composting from a mixture of chicken manure (KA) and chicken feathers (BA) as well as samples of a mixture of cow manure (KS) and chicken feathers (BA), each in a ratio of 1: 1 ( w/w). Bacterial isolation from chicken feather waste and livestock waste had an abundance of 1,4x109 - 16x109 CFU's/gram of compost material. The isolates obtained were 47 isolates that showed proteolytic activity, consisting of 40 isolates coming from a mixture of KS and BA and 7 isolates coming from a mixture of KA and BA. The proteolytic clear zone index ranged from 1.04 0.03 to 4.86 0.73. The 47 isolates obtained had 11 isolates as ideal clear zone index with the percentage 23%. The ideal clear zone index ranges from 4.86 0.73 to 2.40 0.59.

Karakterisasi Morfologi Tanaman Kopi Rakyat di Pulau Bangka Kurnia, Silvi; Ropalia, Ropalia; Zasari, Maera
Jurnal Agro Industri Perkebunan Vol. 11 No. 2 (2023)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v11i2.2717

Coffee is a perennial plant planted on Bangka Island by farmers. Exploration of coffee plants was carried out to see several developed varieties that could be grown on Bangka Island. This study aims to determine the diverse morphological character, phenetic relationship, and variability of folk coffee plants in Bangka. This research was conducted in October 2021-April 2022 in Bangka, Central Bangka and South Bangka. This research was conducted using explorative and descriptive methods. Sampling is carried out randomly to determine the sampling location and data collection. The data from quantitative research were analyzed using variability, while phenetic relationships were analyzed using the UPGMA method (uneweighted pair group method with arithmetic mean) with the NTSYS-pc application (numerical taxonomy software and multivariate analysis system). The results of the analysis of phenetic relationships of qualitative characters resulted in a degree of similarity of coefficients at 53% within coffee of melabun, kota kapur, puput and paku. Analysis of quantitative and combined character phenetic relationships between coffee and puput resulted in a degree of co-efficiency similarity at 25% and 42%. The variability of quantitative character analysis is widespread on plant height and number of flowers per node.

Pertumbuhan Planlet Anggrek Dendrobium sp. pada Media Cacahan Kulit Kayu dan Larutan Rooting Starter Selama Aklimatisasi Nurromadon, Bilham; Ropalia, Ropalia; Zasari, Maera
JURNAL AGROTROPIKA Vol. 23 No. 2 (2024): Jurnal Agrotropika Vol 23 No 2, Oktober 2024
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v23i2.8656

Orchid plantlets are expected to be able to adapt and grow under optimal conditions during the acclimatization period. Selecting appropriate planting media and providing proper nutrition is one of the efforts that can be made to increase the success of high growth of Dendrobium, sp orchid plantlets during the acclimatization period. The aim of this research was to identify the appropriate planting medium and concentration of rooting starter solution to support the growth of Dendrobium sp orchid plantlets during acclimatization. This research was carried out from March to July 2022 at the Bangka Belitung University Experiment and Research Garden. This study used a factorial randomized block plan. The first factor is the planting medium; (M1) pine bark, (M2) Paraserianthes falcataria bark, (M3) acacia bark, and (M4) fern. The second factor is the industrial growth concentration 20 ml/l (K0) and 10 ml/l (K1). The results showed that the acclimatization of Dendrobium sp orchid plantlets was successful as seen from the large growth in plantlet height and the emergence of new shoots. The treatment that produced the highest growth of Dendrobium sp orchid plantlets during the acclimatization period was fern media and the best rooting starter concentration was 20 ml/l. The combination that showed the highest growth in Dendrobium sp. orchid plantlets during the acclimatization period was fern media + 20 ml/rooting starter. Key words: Dendrobium, tree bark, acacia bark, fern, rooting starter, Paraserianthes

Kajian Penggunaan Daun Pisang Kering dalam Pematangan Buah Pisang (Musa Paradisiaca L CV. Kepok) dengan Metode Pemeraman di Lubang Tanah Marhaen, Marhaen; Kusmiadi, Riwan; Ropalia, Ropalia
Jurnal Ilmiah Pertanian dan Peternakan Vol. 1 No. 1 (2023): Juli
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jipper.v1i1.2602

Purpose: This study aimed to examine the effect of dried banana leaves on the ripening process of kapok bananas and evaluate their impact on fruit quality. The research intended to determine whether adding different quantities of dried banana leaves could accelerate ripening, reduce post-harvest losses, and offer a natural, low-cost alternative to chemical ripening agents. Research Methodology: The research was conducted from May to June 2022 at the Agrotechnology Laboratory and Research and Experimental Garden, University of Bangka Belitung. A CRD was used with three treatments: bananas without dried leaves, bananas with 108 g/0.5 m³ of partially dried leaves, and bananas with 216 g/0.5 m³ of fully dried leaves. Each treatment was replicated six times, resulting in 18 experimental units. Observations were made on day 4 and day 6, measuring parameters such as ripening time, fruit hardness, total dissolved solids, vitamin C content, and weight loss. Results: The findings showed that adding dried banana leaves did not significantly affect the ripening time or the fruit quality parameters measured. All treatments reached ripening at the same time, and no substantial differences were observed in vitamin C content, fruit hardness, weight loss. Conclusion: Although dried banana leaves did not significantly speed up ripening, they can help maintain consistent fruit quality and reduce post-harvest losses. Limitations: The study was limited by its small sample size, short observation period, and focus on one banana variety. Contribution: This research offers preliminary evidence of dried banana leaves as a potential natural method for post-harvest banana ripening.

Potensi Oleoresin Gulma Daun Siam (Chromolaena odorata) pada Mortalitas Hama Lalat Buah (Bactrocera cucurbitae) pada Uji in-vitro Ropalia, Ropalia; Apriyadi, Rion; Yurenza, Nadia
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4072

Fruit flies are the main pests that attack various types of fruit vegetables and fruits that can caused a damage in the quality and quantity of production. Fruit flies controlling by chemical insecticides is common to use nowadays, but excessive use of chemical insecticides has a negative impact on the health and environment. One alternative to control the fruit flies is using oleoresins of Chromolaena odorata leaf. This study aims to determine the effect of C. Odorata leaf oleoresin on increasing the mortality of fruit fly pests (Bactrocera cucurbitae). This research used an experimental method with Completely Randomized Design (CRD) which had 6 treatment levels, namely: N0 (Aquadest), N1 (Oleoresins 2%), N2 (Oleoresins 4%), N3 (Oleoresins 6%), N4 (Oleoresins 8%), N5 (Oleoresins 10%), was replicated 4 times so there were 24 experimental units. The results showed that the application of C. Odorata leaf oleoresin had no sigificant effect on mortality percentage and mortality rates, but oleoresin had the potential to increasing fruit fly mortality as concentrations increased. It is necessary to test the increasing concentration of oleoresin > 10% to control fruit fly pests.

The Inhibitory Potential of Botanical Fungicides Against Colletotrichum capsici the Causal Agent of Anthracnose on Chili in-Vitro Sudania, Sudania; Ropalia, Ropalia; Kusmiadi, Riwan
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.44219

Colletotrichum capsici infection in chilies significantly affected the yields. The use of chemical fungicides to control this pathogen has negative effects on health and the environment. The use of botanical pesticides is one alternative to control C. capsici. This study aims to determine the effect of a type of plant extract with a certain concentration to effectively inhibit the growth of the C. capsici The study used a completely randomized design (CRD) with five levels of treatment and five times replication. The treatments were no treatment/negative control, chemical fungicide (contains 70% propineb)/positive control, papaya leaf extract (20%), betel leaf extract (25%), and galangal rhizome extract (30%). Each experimental unit was repeated three times, each repetition using duplo petridishes. The results showed that the treatment had a significant effect on the inhibition of C. capsici in the PDA medium. Botanical fungicide treatment with galangal rhizome extract (30%) was able to significantly inhibit the growth of C. capsici compared to betel and papaya leaf extracts, although it was not as effective as chemical fungicides (contains propinep 70%). The inhibitory abilities of galangal rhizome extract (30%) and chemical fungicide (70% propineb) were 8,34% and 19,91%, respectively.

ISOLASI, KARAKTERISASI, DAN POTENSI ENZIMATIS BAKTERI ASAL TANAH SAMPAH DI TEMPAT PEMBUANGAN AKHIR SEBAGAI KANDIDAT AGEN BIODEKOMPOSER Swandi, Monica Kharisma; Ropalia; Anggraeni
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.2285

Bakteri sebagai organisme dekomposer memiliki kemampuan mendegradasi bahan-bahan organik untuk kerberlangsungan siklus biogeokimia di alam. Berbagai bakteri pendegradasi telah dilaporkan berhasil diisolasi dari berbagai sampel, namun masih terbatas pada potensi enzimatis tertentu sehingga proses dekomposisi tidak optimal. Oleh karena itu, pentingnya penelitian ini dilakukan dengan tujuan mengisolasi, mengkarakterisasi dan mengidentifikasi isolat bakteri penghasil enzim ekstraseluler (selulase, amilase, protease, lipase), dan menganalisis potensi isolat bakteri enzimatis sebagai agen biodekomposer. Tahapan penelitian dimulai dari pengambilan sampel dan isolasi bakteri. Isolat yang diperoleh diuji potensinya sebagai biodekomposer melalui uji aktivitas enzim secara kualitatif, meliputi selulase, amilase, protease, dan lipase. Selanjutnya dilakukan penapisan isolat berdasarkan uji hemolisis. Isolat terpilih kemudian dilakukan penentuan kurva tumbuh, dikarakterisasi dan diidentifikasi. Sebanyak 22 isolat bakteri berhasil diisolasi dari TPA Parit Enam Kota Pangkalpinang. Hasil penapisan menunjukkan sebanyak 8 isolat memiliki kemampuan keempat aktivitas enzim sekaligus, dan dari 8 isolat hanya 4 isolat yang tergolong tidak patogen yaitu isolat BS 2.1, BS 2.2, BS 3.3, dan BS 3.7. Keempat isolat tersebut dapat diproduksi pada fase perbanyakan dalam rentang jam ke-4 hingga ke-18. Berdasarkan karakterisasi morfologi koloni, morfologi sel dan biokimia, isolat BS 2.1, BS 2.2, BS 3.3, dan BS 3.7 dapat diduga sebagai Pseudomonas, Streptococcus, Lactobacillus, dan Bacillus.

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