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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Kedokteran Hewan
Agus Soeprijanto
Faculty of Fisheries and Marine Sciences, University of Brawijaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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INSULIN ASPART CAN LEAD MEIOTIC RESUMPTION IN CATFISH, Clarias Grapienus OOCYTES Syarifuddin, Nurul Wulandari; Warih, Agung Pramana; Soeprijanto, Agus
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.565

Abstract

This study directly tested the hypothesis that the induction of oocyte maturation on catfish Clariasgrapienusby insulin aspartresulted in a transient decrease of oocyte cyclic AMP (cAMP) level due to the increase ofphosphodiesterase (PDE) activity. Under the influence of insulin the germinal vesicle (GV) of the oocyte migrated towards the animal pole (GVM), reached the micropyle, and then dissolved (GVBD). By using different concentrations of insulin aspart i.e., 0 U/ml, 0.01 U/ml, 0.1 U/ml, 1 U/ml and 10 U/ml, optimum amount required was found to be 1U/ml. The number of oocytes incubated for 24 hours with insulin aspartic treatment that undergo meiotic re-initiation can reach the percentage of meiotic resumption oocytes was 82%. The results suggest that catfish oocytes have responsive to sensitive adenylatecyclase and also an active phosphodiesterase system. Addition of the maturation-inducing steroid, a progesterone, stimulated (P0.01) GVBD of catfish oocytes and its combination with insulin aspart showed an additive effect. This information will be important in hormonal manipulation during induced breeding of Indonesian commercial catfish.
The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Anitasari, Septi; Soeprijanto, Agus; Faqih, Abd. Rahem
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1142.131 KB) | DOI: 10.21776/ub.jels.2015.005.02.05

Abstract

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.
Co-Authors Abdul Rahem Faqih Agung Pramana Warih Nurul Wulandari Syarifuddin Septi Anitasari, Septi