Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : EKSAKTA: Journal of Sciences and Data Analysis

Solvent Optimization For Genistein Isolation Of “Rotten Tempe” By High Performance Liquid Chromatography Method Cucun Alep Riyanto; Hartati Soetjipto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 17, ISSUE 2, August 2017
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol17.iss2.art3

Abstract

Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extraction process by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio. Where as the highest contents of genistein from the 4th day “rotten tempe” as 26.199 ± 25.146 (mg/g).
Extraction and Chemical Compounds Identification of Red Rice Bran Oil Using Gas Chromatography – Mass Spectrometry (GC-MS) Method Hoo Sheren Hartono; Hartati Soetjipto; A. Ign. Kristijanto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 17, ISSUE 2, August 2017
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol17.iss2.art2

Abstract

The objectives of the study are to obtain optimum yield of extraction red rice bran oil, to determine the physico-chemical characteristics, and componen coumpounds. Data was analyzed using Nir Parametric Statistics by Friedmann test. The result showed the optimum extraction results was obtained by the ratio of substrate : solvent of 1: 8 and the oil yield was 12.31 ±0.325%. The physico properties of red rice bran oil were greenish brown colour, with a density ranged from 0.908 ± 0.014 to 0.922 ± 0.014 (g/mL), and the water content ranged from 0.87 ± 0.06 to 0.91 ± 0.02 %. The chemical properties of red rice bran oil were: the acid number ranged from 116.41 ± 1.22 to 118.11 ± 2.45 (mg NaOH/g); the saponification number ranged from 193.74 ±21.88 to 199.62 ± 12.63 (mg KOH/g); and the peroxide number ranged from 24.37 ± 2.44 to 26.07 ± 4.88 (mgek/kg), respectively. Oils was analyzed used GC-MS. The chemical components of rice bran oil are oleic acid (46.24%), palmitic acid (18.25%), linoleic acid (13.29%), 9-octadecane(7.76%).