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Journal : EduChemia: Jurnal Kimia dan Pendidikan

Linoleic Acid Isolation from Watermelon Seeds Oil (Citrullus lanatus L) with Urea Inclusion Method Divina Anindya Della; Hartati Soetjipto; November Rianto Aminu
EduChemia (Jurnal Kimia dan Pendidikan) Vol 7, No 2 (2022)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/educhemia.v7i2.15128

Abstract

Nutritional problems still become a crucial issue in Indonesian public health. Fulfillment of nutritional balance is expected to improve life quality. In humans, one of the nutrients that play a role in the growth of brain and nervous system functions is PUFA, such as omega-6 and omega-3. Besides, fulfillment of nutritional balance can prevent some diseases; especially linoleic acid used to prevent some diseases. Linoleic acid is an omega-6 and essential fatty acid. Linoleic acid is the main essential fatty acid because it is the precursor for GLA, DGLA, and AA. Omega-6 works with omega-3 and some omega-3 benefits are supported or can arise by omega-6 presence. Studies reveal that watermelon contains high linoleic acid. This study aimed to determine the optimum temperature and ratio of fatty acids: urea for the isolation of linoleic acid from watermelon seed oil. The method used was urea inclusion with a variety of temperatures (-6oC, 6oC, 18oC) and the ratio of fatty acid: urea (1:1; 1:3; 1:5). The results showed the optimum watermelon seed oil linoleic acid isolate at 18oC and the ratio of fatty acids: urea 1:5 with linoleic acid purity (%) of 82.53%.
Testing The Antibacterial Activity of Crude Extracts of Tithonia diversifolia Leaves, Flowers, and Roots in Various Solvent Fraction Indah Tri Susilowati; Hartati Soetjipto; Susanti Pudji Hastuti
EduChemia (Jurnal Kimia dan Pendidikan) Vol 7, No 1 (2022)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/educhemia.v7i1.12403

Abstract

Medicinal plants are natural sources of effective antimicrobials from natural products. Tithonia diversifolia (Asteraceae) is a conventional herb that can be used for the treatment of malaria, wounds, and other diseases with biological properties such as antioxidant, antimalarial, antidiabetic, antibacterial, and anti-hyperglycemic. Research on Tithonia diversifolia leaf extract as an antibacterial has been widely carried out. However, its use as a natural antibacterial is rarely studied, especially on flowers and roots. This study aimed to test the antibacterial activity of leaf, flower, and root extracts of the Tithonia diversifolia plant in various solvent fractions. The methods used in this research were Antibacterial Testing with Agar Diffusion and Direct Bioautography Methods, while the solvent fractions used were petroleum ether, chloroform, and ethyl acetate. The results showed that the crude extract of Tithonia diversifolia leaves, flowers, and roots for petroleum ether, chloroform, and ethyl acetate fractions contained antibacterial compounds. They could inhibit the growth of E. coli, P. aeruginosa, S. aureus, and B. subtilis bacteria, with inhibitory activity in chloroform fraction, which was better than in petroleum ether and ethyl acetate fractions. The results of the bioautography test showed that the inhibition was good on gram-positive bacteria with the highest number of spots in the chloroform fraction for the crude leaf and flower extract samples.