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Sri Nanan Widiyanto
School of Life Sciences and Technology, Bandung Institute of Technology, Bandung
Mathematics

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Clonal Propagation of Broccoli, Brassica oleracea L.var. italica through In Vitro Shoot Multiplication Sri Nanan Widiyanto; Dwi Erytrina
Jurnal Matematika & Sains Vol 6, No 2 (2001)
Publisher : Institut Teknologi Bandung

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Shoot-tips of broccoli, Brassica oleracea var. italica cv. Green Valiant, were used as explant source in in vitro clonal propagation. The objective of this research is to examine the influence of growth regulators on axillary shoot induction and root formation. Shoot-tips were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog. Benzyl adenine (BA) and kinetin (KIN) at 0.10-10.0 μM were used either singly or in combination with naphthalene acetic acid (NAA) at 0.01-10.0 μM to induce shoot multiplication. For inducing root formation, NAA, indole acetic acid (IAA) or indole butyric acid (IBA) at 0.10-10.0 μM were applied. Experimental results showed that the addition of BA at 10.0 μM induced axillary shoot proliferation respectively. The addition of KIN induced less shoot multiplication than that of BA. During a four-week period of subculture, a single shoot-tip of broccoli produced 16-20 shoots on medium with the addition of 10.0 μM BA. Elongated shoots were mostly rooted on medium containing 1.0 μM NAA. Plantlets were successfully transferred to a soil-sand mixture medium (1:1) and grown naturally in the green house.
Effect of Cytokinins and Carbenicillin on In vitro Axillary-Shoot Growth of Albizia [Albizia falcataria (L.) Fosberg] Sri Nanan Widiyanto; Mirah Dila Sari; Rina Ratnasih Irwanto
Jurnal Matematika & Sains Vol 13, No 2 (2008)
Publisher : Institut Teknologi Bandung

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Effect of N6-benzylaminopurine (BA), 6-furfurylaminopurine (kinetin) and carbenicillin on axillary-shoot growth ofalbizia [Albizia falcataria (L.) Fosberg] from cotyledonary node-segments were evaluated in vitro. Shoot inductionmedium was composed of Murashige and Skoog (MS) basal medium with or without the addition of BA or kinetin. Thesensitivity to carbenicillin was tested using shoot induction medium with the addition of carbenicillin at 10-50 mg/L.The results showed that the optimal axillary-shoot growth was observed on medium containing 2.2 μM BA and 2.3 μMkinetin, which reached the average of 36.0±2,2 mm shoot-length and 2.9±1,1 nodes/shoot in four weeks. The addition ofcarbenicillin inhibited the axillary-shoot growth, shown by 6.1-11.5 mm of shoot length of and 1.0-11.1 of nodes/shoot.A total of 60 shoots were elongated and rooted in in vitro condition on MS medium without growth regulator. Allplantlets were successfully transferred to a soil-sand medium (1:1) and grew well in the green-house.
Co-Authors Dwi Erytrina Mirah Dila Sari Rina Ratnasih Irwanto