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All Journal Jurnal Matematika & Sains Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal 3BIO: Journal of Biological Science, Technology and Management
Sri Nanan Widiyanto
School Of Life Sciences And Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Immunology & microbiology Mathematics Neuroscience

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Clonal Propagation of Broccoli, Brassica oleracea L.var. italica through In Vitro Shoot Multiplication Sri Nanan Widiyanto; Dwi Erytrina
Jurnal Matematika & Sains Vol 6, No 2 (2001)
Publisher : Institut Teknologi Bandung

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Abstract

Shoot-tips of broccoli, Brassica oleracea var. italica cv. Green Valiant, were used as explant source in in vitro clonal propagation. The objective of this research is to examine the influence of growth regulators on axillary shoot induction and root formation. Shoot-tips were excised from sterile germinated seedlings and placed on shoot induction medium containing basal salts of Murashige and Skoog. Benzyl adenine (BA) and kinetin (KIN) at 0.10-10.0 μM were used either singly or in combination with naphthalene acetic acid (NAA) at 0.01-10.0 μM to induce shoot multiplication. For inducing root formation, NAA, indole acetic acid (IAA) or indole butyric acid (IBA) at 0.10-10.0 μM were applied. Experimental results showed that the addition of BA at 10.0 μM induced axillary shoot proliferation respectively. The addition of KIN induced less shoot multiplication than that of BA. During a four-week period of subculture, a single shoot-tip of broccoli produced 16-20 shoots on medium with the addition of 10.0 μM BA. Elongated shoots were mostly rooted on medium containing 1.0 μM NAA. Plantlets were successfully transferred to a soil-sand mixture medium (1:1) and grown naturally in the green house.
Effect of Cytokinins and Carbenicillin on In vitro Axillary-Shoot Growth of Albizia [Albizia falcataria (L.) Fosberg] Sri Nanan Widiyanto; Mirah Dila Sari; Rina Ratnasih Irwanto
Jurnal Matematika & Sains Vol 13, No 2 (2008)
Publisher : Institut Teknologi Bandung

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Effect of N6-benzylaminopurine (BA), 6-furfurylaminopurine (kinetin) and carbenicillin on axillary-shoot growth ofalbizia [Albizia falcataria (L.) Fosberg] from cotyledonary node-segments were evaluated in vitro. Shoot inductionmedium was composed of Murashige and Skoog (MS) basal medium with or without the addition of BA or kinetin. Thesensitivity to carbenicillin was tested using shoot induction medium with the addition of carbenicillin at 10-50 mg/L.The results showed that the optimal axillary-shoot growth was observed on medium containing 2.2 μM BA and 2.3 μMkinetin, which reached the average of 36.0±2,2 mm shoot-length and 2.9±1,1 nodes/shoot in four weeks. The addition ofcarbenicillin inhibited the axillary-shoot growth, shown by 6.1-11.5 mm of shoot length of and 1.0-11.1 of nodes/shoot.A total of 60 shoots were elongated and rooted in in vitro condition on MS medium without growth regulator. Allplantlets were successfully transferred to a soil-sand medium (1:1) and grew well in the green-house.
Correlation of Microclimate of West Java on Caffeine and Chlorogenic acid in Coffea canephora var. robusta Suci Awaliyah; Sri Nanan B. Widiyanto; Rijanti R. Maulani; Asep Hidayat; Ujang Dinar Husyari; Tati Suryati Syamsudin; Erly Marwani
3BIO: Journal of Biological Science, Technology and Management Vol. 4 No. 1 (2022)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2022.4.1.7

Abstract

Caffeine and chlorogenic acid (CGA) are two compounds that play a role in determining the quality of coffee. The amount of the two compounds may vary depending on the environment where they are grown. This study aimed to determine the correlation between the local microclimatic condition and the concentration of caffeine and CGA in green and roasted beans of Robusta coffee from six different cultivation areas in West Java, Indonesia (i.e., Ciamis, Tasikmalaya, Sumedang, Kuningan, Cianjur, and Bogor). Samples of green beans and roasted beans were extracted with 70% methanol for caffeine analysis and ethyl acetate for CGA analysis. Caffeine and CGA were analyzed by UV-HPLC using a C18 shimpack gist shimadzu column, with an isocratic elution of methanol:water (1:1) at a 1 mL/min flow rate. Detection was performed at  λ272 nm and λ324 nm for caffeine and chlorogenic acid, respectively. Principal component analysis (PCA) was used to evaluate the correlation between microclimate with caffeine and chlorogenic acid. Results indicated that the concentration of caffeine ranged from 7.67 to 16.52% and 10.79 to 15.56% in the green and roasted bean coffee, respectively. The concentration of CGA ranged from 0.74 to 3.03% and 0.25 to 0.77% in the green and roasted bean coffee, respectively. Based on PCA analysis, the most influential microclimate on the caffeine concentration were the humidity, temperature, and altitude, with the total variance of PC1 and PC2 of  76.3%. However, there was no positive correlation between the measured microclimate and the CGA concentration. In conclusion, Robusta coffee's caffeine content is positively affected by the microclimatic condition (i.e., humidity, temperature, and altitude).
Transfer Gen Penanda Pada Kentang (Solanum tuberosum L.) CV Panda dan Atlantik dengan Bantuan Agrobacterium Noor Aini Habibah; Sri Nanan B
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.91

Abstract

This research was carried out to evaluate the efficiency of marker gene transfer of potato (Solanum tuberosum L.) explant tissues, which were co-cultivated with Agrobacterium tumefaciens strain LBA 4404 carrying binary vector pBI121. Nodes and leaf segments from shoot cultures of Panda and Atlantic cultivars were used as explants. The marker gene transfer procedure was initiated with explant pre-conditioning, followed with co-cultivating and selecting of putative transformant cells. Explants were pre-conditioned over-night in callus induction medium containing basal medium of MS (Murashige & Skoog, 1962) supplemented with 5 mg/l naphthalene acetic acid (NAA) and 0.1 mg/l benzyl adenine. After 10 min. inoculation in  A. tumefaciens suspension, explants were co-cultivated for 1, 3, 5, 7 and 9 days. After co-cultivation, explant tissues were placed on callus induction medium added with 150 mg/l kanamycin. Successful marker gene transfer was calculated as the percentage of total explants producing callus on selection medium. Results showed that more co-cultivated nodal explants became resistant to kanamycin than co-cultivated leaf explants. The highest efficiency of transformation (45%) of nodal explants was observed in Panda cultivar whereas that in Atlantik was only 30%. The most efficient transformation of leaf explants was observed in Panda cultivar (20%) whereas that in Atlantik was15%. The PCR analysis detected the presence of NPTII gene in transgenic tissues.
Co-Authors Asep Hidayat Dwi Erytrina Erly Marwani, Erly Mirah Dila Sari Noor Aini Habibah Rijanti R. Maulani Rina Ratnasih Irwanto Suci Awaliyah Tati Suryati Syamsudin Ujang Dinar Husyari