Nanik Sriyani
Jurusan Agroteknologi Fakultas Pertanian Universitas Lampung

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Penggunaan Metode Bioassay untuk Mendeteksi Pergerakan Herbisida Pascatumbuh Paraquat dan 2,4-D dalam Tanah Sriyani, Nanik; Salam, Abdul Kadir
JOURNAL OF TROPICAL SOILS Vol. 13 No. 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.199-208

Abstract

A simple and cheap method to detect herbicide residue in soil and water is urgently needed as the quantity and frequency of herbicide usage is steadily increasing in Indonesia which raises concern about the effects of herbicide residue in soil and water.  This study is the third step from a series of studies aim to develop bioassay technique to detect the present and quantity of herbicides in soil and water.  In this study, bioassay was used to detect movement of paraquat and 2,4-D herbicides in soil.  Study was carried out using soil column method.  Treatments were arranged factorially in a completely randomized block design with 3 replicates.  Two ultisol soil types: Podsolik Merah Kuning (PMK) and Latosol Coklat (LC) and 2 post emergence herbicides: paraquat and 2,4-D, were tested.  To calculate the amount of herbicide using bioassay, each standard curve for paraquat and 2,4-D were developed.  Using these standard curves, the amount of paraquat and 2,4-D was calculated based on the growth rate of caisim as indicator plant.  Results showed that bioassay method can be utilized to detect herbicide movement in soil.  The amount and the rate of herbicide movement were determined by soil and herbicide types.  In PMK, paraquat reached the depth of 20-30 cm at 2 weeks after application (WAA), however, after that the amount of paraquat found was very limited.  In LC, the movement of paraquat was more limited compared to its movement in PMK.  Paraquat reached soil depth of 10-20 cm at 2 WAA, afterward paraquat was only detected in soil depth of 0-10 cm.  Similar pattern was observed for 2,4-D which reached soil depth of 30-40 cm at 1 WAA in PMK.  In LC, 2,4-D movement was more limited.  At 2 WAA, 2,4-D in LC reached soil depth of 30-40 cm in limited amount and after 12 WAA the herbicide was detected only at soil depth of 0-10 cm.
EFIKASI DAN UJI SIFAT CAMPURAN HERBISIDA SAFLUFENACIL DAN TRIFLUDIMOXAZIN TERHADAP GULMA Ageratum conyzoides, Digitaria ciliaris, DAN Cyperus kyllingia Cahyani, Dwi; Sembodo, Dad Resiworo Jekti; Sriyani, Nanik; Pujisiswanto, Hidayat; Susanto, Herry
JURNAL AGROTROPIKA Vol. 24 No. 2 (2025): Jurnal Agrotropika Vol 24 No 2, Oktober 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i2.11687

Abstract

This study aimed to evaluate the efficacy of single herbicides saflufenacil, trifludimoxazin, and their mixtures, as well as to determine the interaction characteristics of the saflufenacil and trifludimoxazin combination in weed control. The experiment was conducted in a Plastic House in Natar District, South Lampung Regency, from April to May 2025. A single-factor Randomized Block Design (RBD) was applied with 54 treatment combinations, consisting of 3 herbicide types at 6 dosage levels: single saflufenacil (0, 25, 50, 100, 200, and 400 g/ha), single trifludimoxazin (0, 12.5, 25, 50, 100, and 200 g/ha), and their mixtures (0, 37.5, 75, 150, 300, and 600 g/ha). Treatments were applied to 3 weed species: the broadleaf Ageratum conyzoides, the grass Digitaria ciliaris, and the sedge Cyperus kyllingia. Each treatment was replicated 6 times. The interaction analysis of herbicide mixtures was carried out using the Multiplicative Survival Model (MSM), since the two herbicides have different modes of action. The results showed that: (1) single saflufenacil was only effective in controlling Ageratum conyzoides at (25-400 g/ha). Single trifludimoxazin effectively controlled Ageratum conyzoides (12.5-200 g/ha), Digitaria ciliaris (200 g/ha), and Cyperus kyllingia (50-200 g/ha). The mixture of saflufenacil+trifludimoxazin effectively controlled Ageratum conyzoides (37.5-600 g/ha), Digitaria ciliaris (75-600 g/ha), and Cyperus kyllingia (300-600 g/ha). (2) With an expected LD50 value of 83.36, which was higher than the observed LD50 value of 67.33, a co-toxicity coefficient of 1.24 (>1) was obtained, indicating that the mixture exhibited a synergistic effect.