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All Journal Tropical Medicine Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Yanri W Subronto
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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The Profile of Interferon-γ (IFN-γ) and Interleukin-10 (IL-10) in Pulmonary Tuberculosis Patients Widya Wasityastuti; Yanri W Subronto; Marsetyawan HNE Soesatyo
Tropical Medicine Journal Vol 1, No 1 (2011): Tropical Medicine Journal
Publisher : Pusat Kedokteran Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/tmj.4563

Abstract

Introduction: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis and becomes the main health problems in the world and in Indonesia, as well. The microorganism itself is an intracellular pathogen. The control of tuberculosis infection depends on cell-mediated immunity involving activated macrophages, T cells, and cytokines. The balance and dynamic changes between Th1 cytokine and Th2 cytokine refl ect the immune response of host and infl uence the clinical manifestation of the disease.Objectives: This research was designed to study the profi le and interaction of IFN-γ (Th1 cytokine) and IL-10 (Th2 cytokine) of pulmonary tuberculosis (PTB) patients in endemic area.Methods: Peripheral blood mononuclear cells of 23 pulmonary TB patients and 16 healthy persons was cultured and stimulated by phytohaemagglutinin (PHA) to investigate the ability to secrete IFN-γ and IL-10.Result: The result showed that there was a decreased of IFN-γ response to PHA in PTB patients, suggesting the defi ciency of general immune capacity in PTB. In contrast, IFN-γ secreted by specifi c antigen was higher in PTB patients which minimal lung lesion was higher than moderate-far advanced. It is related to IFN-γ roles as immunomodulator in cellular immunity and immunoprotectant through stimulated antimicrobial capacity in macrophage. In fact, IL-10 response to PHA and M.tuberculosis antigen in PTB patients was lower than that of in healthy persons; moderate-far advanced lung lesion was the lowest. It was probably refl ecting their poor general conditions. Paired distribution between IFN-γ and IL-10 pointed out the leaning of negative interaction. It refl ected the existence of counterpart/cross regulation between IFN-γ(Th1 cytokine) and IL-10 (Th2 cytokine).Conclusion: In conclusion that specifi c immune response of PTB is related to the degree of lung lesion. This study also provides the balance of Th1 cytokine and Th2 cytokine in relation to TB.Key words: tuberculosis, immune response, IFN-γ, IL-10, lung lesion
VIROLOGICAL AND IMMUNOLOGICAL RESPONSE TO ANTIRETROVIRAL TREATMENT IN HIV-INFECTED PATIENTS (Respons Virologis dan Imunologis terhadap Pengobatan Anti-Retroviral di Pasien Terinfeksi HIV) Umi S. Intansari; Yunika Puspa Dewi; Mohammad Juffrie; Marsetyawan HNE Soesatyo; Yanri W Subronto; Budi Mulyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1187

Abstract

Infeksi HIV/AIDS masih menjadi tantangan global. Pengobatan antiretroviral (ART) berperan dalam menurunkan replikasi virus,menurunkan tingkat kematian infeksi oportunistik dan meningkatkan kualitas hidup orang yang hidup dengan HIV/AIDS. Meskipundemikian data terkait respons virologis dan imunologis termasuk aktivasi imun masih sangat terbatas. Penelitian ini bertujuan untukmengetahui respons virologis dan imunologis pasien HIV setelah 6 bulan memulai pengobatan ARV. Subjek dari penelitian observasionalprospektif ini adalah 44 pasien HIV yang belum pernah mendapat pengobatan ARV, yang berobat di RSUP Dr. Sardjito Yogyakarta danRSUP Dr. Kariadi, Semarang. Sampel darah EDTA sebanyak 6 mL diambil pra dan pasca 6 bulan pengobatan ARV untuk pemeriksaanjumlah sel T CD4+, kadar RNA HIV dan persentase sel T CD8+/38+. Kadar RNA HIV turun secara bermakna sejalan dengan persentasesel T CD8+/38+, sementara jumlah sel T CD4+ meningkat bermakna. Sebanyak 79,5% pasien mengalami pemulihan sel T CD4 optimal(>50 sel/μL) dan kadar RNA HIV turun lebih dari 1 log10 kopi/mL pada 93% pasien. Pasien dengan respons tidak sesuai antara virologisdan imunologis didapatkan sebanyak 13,6%. Kadar HIV bernasab positif dengan persentase sel T CD8+/38+ (r=0,58, p<0,0001) danbernasab negatif dengan jumlah sel T CD4+ (r=–0,470 (p<0,0001). Berdasarkan telitian ini, sebagian besar pasien mempunyai responsvirologis dan imunonologis yang sesuai 6 bulan setelah ART. Sebanyak 20,45% pasien tidak berespons atau mengalami ketidaksesuaianrespons virologis dan imunologis dan memerlukan penilaian dan pengobatan secara terus menerus.
Co-Authors Anggorowati, Nungki Budi Mulyono Ghozali, Ahmad Marsetyawan HNE Soesatyo Mohammad Juffrie Sulistyaningsih, Rita C Umi S. Intansari Widya Wasityastuti Yunika Puspa Dewi