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Bimo Budi Santoso
Jurusan Kimia FMIPA UNIPA
Agriculture, Biological Sciences & Forestry Chemistry Mathematics Physics

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PEMBUATAN DAN ANALISIS BIO-ETANOL DARI PATI SAGU (Metroxylon sago) ASAL PAPUA Bimo Budi Santoso; Dyah Novitasari; Prawatya Istalaksana
Jurnal Natural Vol. 9 No. 1 (2010)
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v9i1.33

Abstract

Preparation and bio-ethanol analysis of sago (M. sago) starch from Papua have been carried out. The preparation of bioethanol was conducted by hydrolysis, fermentation, distillation and purification. The length of fermentation applied is 3, 4, 5, 6, 7 and 8 days. Bioethanol then was analyzed qualitatively and quantitatively. Qualitative analyses were boiling point and molecular weight determination. Quantitative analyses were determination of the volume of bioethanol content and the composition of bioethanol of every length of fermentation. Based on the qualitative analyses, the samples obtained was ethanol with boiling point of 79 oC and molecular weight of 46 gram/mol. Whereas, based on the quantitative analyses, the more the length of fermentation, the more volume of ethanol obtained and the composition of ethanol increased, with the optimum of the fermentation length was 8 days and the volume ethanol obtained was 123.5 mL, in addition the composition of ethanol at fermentation length of 8 days was 100%.
SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI Bimo Budi Santoso
Jurnal Natural Vol. 7 No. 1 (2008): Jurnal Natural
Publisher : FMIPA Universitas Papua

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Abstract

Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed. The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents, A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R2= 0,77) and methanol (R2 = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher
ISOLASI DAN KARAKTERISASI ZAT WARNA Capsicum frutescens Bimo Budi Santoso
Jurnal Natural Vol. 1 No. 2 (2002): Jurnal Natural
Publisher : FMIPA Universitas Papua

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Abstract

Isolation and characterization of pigments from C. frutescens have been done. Three components (pigments) were eluted using column chromatography namely yellow pigment, orange pigment, and red pigment. Solvents used to separate the pigments were hexane/dichloroethane, dichloroethane, dichloroethane/methanol and chloroform/dichloroethane. Isolated pigments were subjected to TLC to prove the purity and to get the Rf value of the isolates. Then, the pure isolates were subjected to UV spectroscopy to get UV spectrum. Base on the data of polarity, pigment color, Rf value, and UV spectrum, the isolated components of C. frutescens are probably b-carotene (yellow pigment), phenolic or flavonoid compound (orange pigment), and capsanthin (red pigment).
UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth) Restianti Restianti; Bimo Budi Santoso; Maria Ludya Pulung
Jurnal Natural Vol. 16 No. 2 (2020): Jurnal Natural
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v16i2.114

Abstract

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to E. coli of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli and a hexane extract has a weak inhibition to bacteria S. aureus.
AKTIVITAS ANTIBAKTERI DAN KOMPONEN KIMIA EKSTRAK HEKSAN, ETIL ASETAT DAN ETANOL BATANG BROTOWALI (Tinospora crispa Linn) ASAL MANOKWARI Bimo Budi Santoso; Argina Argina; Alfhons D. Sirampun
Jurnal Natural Vol. 16 No. 2 (2020): Jurnal Natural
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v16i2.115

Abstract

Antibacterial activity test and chemical component analysis of hexane, ethyl acetate and ethanol extract of Brotowali stem from Manokwari Regency against B. subtillis and E. coli have been carried out. The extraction of the T. crispa stems was carried out by the maceration method in stages based on the polarity of the solvent. Chemical component analysis was performed using GC-MS and phytochemical tests. Based on the identification of chemical compounds by phytochemical screening, it shows the presence of flavonoids in the hexane extract, while in the ethyl acetate and ethanol extracts there are alkaloids, flavonoids, saponins, and tannins. The results of the chemical component analysis of hexane, ethyl acetate and ethanol extracts with GC-MS showed that the hexane extract contained 55 chemical components with 11 main components, ethyl acetate extract consisted of 39 components with 8 main components and ethanol extract there were 57 components with 8 main components. The results of the antibacterial activity test using the well method showed that the hexane and ethyl acetate extracts showed better antibacterial activity than the ethanol extract against the two types of tested bacteria, namely E. coli and B. subtillis. The antibacterial activity of hexane and ethyl acetate extracts against E. coli was 11.00 and 14.00 mm, respectively and against B. subtillis were 7.00 and 7.50 mm, respectively.
Co-Authors Alfhons D. Sirampun Argina Argina Dyah Novitasari Maria Ludya Pulung Prawatya Istalaksana Restianti Restianti