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All Journal Jurnal Biologi Tropika NICHE Journal of Tropical Biology

Rully Rahadian

Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University Jl. Prof. H. Soedarto, SH., Tembalang Semarang 50275 Indonesia

Author-ID : 3876308

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles

Uji efikasi beberapa isolat bakteri entomopatogen terhadap kecoa (Orthoptera) Periplaneta americana (L.) dan Blatella germanica (L.) dalam skala laboratorium Monica Sekar Rini; Rully Rahadian; Mochammad Hadi; Deni Zulfiana
Jurnal Biologi Tropika Vol.1, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Kecoa merupakan salah satu serangga vektor penyakit yang dapat menimbulkan dampak buruk pada kesehatan manusia. Pengendalian kecoa menggunakan insektisida yang berlebihan dapat menimbulkan residu di lingkungan dan resistensi kecoa. Oleh sebab itu perlu dilakukan pengendalian alternatif diantaranya dengan menggunakan agen hayati berupa bakteri entomopatogen. Pada penelitian ini digunakan tiga isolat bakteri entomopatogen yang masing-masing diisolasi dari Spodoptera litura mati, dan Bacillus thuringiensis koleksi IPBCC. Pengujian dilakukan dengan metode semprot dan umpan pada konsentrasi 108. Hasil penelitian menunjukkan bahwa ketiga isolat bakteri entomopatogen yang digunakan pada penelitian ini dapat menimbulkan mortalitas kecoa. Pengamatan morfologi dan uji Postulat Koch menunjukkan bahwa kematian kecoa dipastikan disebabkan oleh isolat bakteri yang diberikan. Pada metode semprot, isolat bakteri SP4 menyebabkan mortalitas tertinggi baik pada P. americana (26,67%) maupun pada B. germanica (80%). Sedangkan pada metode umpan, isolat B. thuringiensis menyebabkan mortalitas tertinggi pada P. americana (10%) dan pada B. germanica (6,67%). Dari segi waktu kematian, diketahui bahwa perlakuan SP4 metode semprot lebih efektif dalam memengaruhi mortalitas B. germanica yaitu 2 jam 30 menit 46 detik.Kata kunci: Pengendalian hayati, bakteri entomopatogen, uji efikasi

Struktur komunitas makroarthropoda tanah di lahan pertanian durian di kawasan Gunungpati Febri Edo; Udi Tarwotjo; Rully Rahadian
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this study is to compare community structures, which include diversity, abundance, evenness, and macroarthropod dominance of monoculture durian farmland, polyculture durian farmland and non-durian farmland (arabica coffee) in Gunungpati region. This research is an exploratory observation carried out from February to March 2019. Samplingwas done using pitfal trap. The identification of samples was carried out in the Diponegoro University Ecology and Biosystematics laboratory. Data analysis was carried out by calculating diversity index (H'), evenness (e), dominance (D), richness, Sorenson test and Hutcheson t-test. The results showed that arthropods in monoculture farmland were found 195 individuals, in polyculture farmland were found 181 individuals and in arabica coffee farmland were found 229 arthropods. Diversity index (H') and evenness (e) in monoculture farmland were lower than in polyculture farmland and arabica coffee farmland. The three areas are dominated by the family Formicidae. The three fields have a high degree of similarity. Overall 8 families were found as predators, 4 families as herbivores, 3 families as detritivores and 1 family as decomposers.

Struktur komunitas mikroartropoda tanah di lahan pertanian kentang di Desa Sembungan Dataran Tinggi Dieng, Jawa Tengah Insan Shiddiq; Rully Rahadian; Udi Tarwotjo
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The use of manure in the area of Dieng Plateau is likely to increase from year to year. Excessive use of fertilizer has the potential to disturb soil fauna. The objective of this research are to know the effect of intensively use of pure chicken manure and chicken manure in combination with husk in the Dieng Plateau potato farm, Sembungan Village to structure of soil microarthropods. Sampling was located in two station of potatoes farmland that uses pure chicken manure (chicken manure: CM) and chicken manure in combination with husk (chicken manure + husk :CMS). Soil sampling was applied using transect line method with five sampling points. The structure of soil microarthropods communities was analysed into relative abundance, the Shannon-Wiener’s diversity index, the evenness index, the Sorensen’s similarity index, and Hutcheson’s t-test. The results showed that the density of soil microarthropods on potato farmland using chicken manure in combination with husk (5620 individuals/m2) was higher than farmland using pure chicken manure (1830 individual/m2). The dominant taxa in both farmland are Acari and Collembola. Based on the index of Sorensen similarities, the composition of soil microarthropods in both farmlands is relatively similar (76%). Statistically, the diversity of soil microarthropods between the two different potatoes farmland is significantly different.

Distribusi spasial mikroartropoda tanah berdasarkan jarak yang berbeda dari Pantai Utara Kota Semarang, Jawa Tengah Anggi Aprilia Budi Prasetiani; Rully Rahadian; Mochamad Hadi
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Soil microarthropods are soil mesofauna which has body size 0.02 - 0.17 mm. Distribution of soil fauna of an area depends on the condition of environment and the characteristic of biologic. The objective of this research is to determine the distribution of spatial and structure community of soil microarthropods in several locations that have different distance from the northern coast of Semarang City. The research was conducted at December 2017 until June 2018. Sampling was conducted at three different locations, that is area around of Tawang Station, the Undip Pleburan Campus area, and the Cemoro Songo Park area. Samples were extracted and identified at the Ecology and Biocytic Laboratory, and analysis of soil and C-organic material at the Soil Mechanics Laboratory and Environmental Laboratory. The results of identification found 53 species consist of 14 ordo and 11 families. The distribution of all microarthropods are almost included in clumped distributions. The abundance of microarthropods is dominated by Acari and Collembola. The evenness are included in the highest evenness categories with the highest location with a value of 0.93. The similarity of taxa are included in the lowest categories with a value of 10.26% -19.35%.

Pola sebaran dan preferensi habitat kodok buduk (Duttaphrynus melanostictus Schneider, 1799) di Kecamatan Tembalang, Semarang Zuni Miftakhurrohmah; Karyadi Baskoro; Rully Rahadian
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Asian common toad is a human-commensal organism that can easily be found around. This species is the most common species that has a very wide habitat range, from backyard to secondary forest. Tembalang has relatively diverse habitat type which will be suitable for Asian common toad's habitat, but, as the land use of the area are changing from rural to urban land use, the toad population is changing as well. The objectives of the research are to understand the distribution pattern of Duttaphrynus melanostictus along with its habitat preference in Kecamatan Tembalang, Semarang. The research was taken in March – July 2019, by observing the toad population in three different types of habitat (developed habitat, vegetation-covered habitat, and open habitat). The data was taken using visual encounter survey method, at night starting from 7 to 10 p.m. The data of toad's prevalence was analyzed using Morisita index to comprehend the distribution pattern, while the habitat variable data was analyzed using Ward's Cluster analysis using PAST software. From the research, can be concluded that the Asian common toad is distributed with clumped pattern in Tembalang, with Morista index of 7.23, without any specific preference to habitat's type of substrate. The distribution pattern mostly influenced by the macro environmental condition, such as vegetation cover, elevation, buffer distance to nearest river (or water reservoir), and temperature.

Community structure of soil microarthropods in active and passive zones of Ngronggo landfill Salatiga Psn Masruri Sulistiyanto Ari; Rully Rahadian; Udi Tarwotjo
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Soil microarthropods play a key role in decomposing organic materials. The objectives of this study were to compare the structure of soil microarthropods communities in the active and passive zones of Ngronggo landfill Salatiga. The sampling of ten soil samples in each active and passive zones was performed for soil microarthropods extraction and soil physical-chemical analysis. Soil microarthropods were extracted using modified Barlese-Funnel. Soil microarthropods data were analyzed to determine abundance, diversity, and similarity. Physical-chemical factors were measured, including soil texture, heavy metal content (Pb, Cd, Cu, Zn, and Hg) and organic matter. The results show that all attributes of structure of soil microarthropods communities in active zone are higher than in passive zone, i.e., the density, the species richness, the species diversity, and the evenness. In both zones, the composition of soil microarthropods tend to be different.. Organic matter is likely the most important soil physicochemical factors to form the microarthropod structure in active and passive zones. This study contributes a new information about the role of soil microarthropods in decomposing the disposal waste of material and it would be benefit for developing a bioindicator of waste decomposition in landfill area.

Community structure of algae epiphytic in different leaf litter of mangroves Riche Hariyati; Sri Widodo Agung Suedy; Rully Rahadian
NICHE Journal of Tropical Biology Vol. 1, No. 2, Year 2018
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Mangrove is a plant that has the morphological and physiological adaptations to habitats which influenced by a tide. This plant can grow and develop in an environment of high salinity and its soil conditions are less stable and aerobic. Litter mangrove is the main source for the availability of detritus and is the largest component constituent of a food web in a mangrove ecosystem environment. Algae epiphytic microalgae is a group that lives attached to the plant, including the mangrove litter. The study aims to assess the abundance and diversity of epiphytic algae on different types of mangrove leaf litter. The observed mangroves leaf litter were Avicennia alba, Sonneratia sp. and Rhizophora sp. Epiphytic algae were brushed on mangrove leaf litter covering an area of 6.25 cm2 and diluted with distilled water. Samples were observed under a microscope with a magnification of 400 x. Water quality parameters were measured in situ and the organic ingredients, total N and P were analyzed. The results showed that epiphytic algae on each type of mangrove leaf litter were not different. Rhizophora sp is the most abundant and the richest species compared with other types of litter that are equal to 622 individuals / cm2 and 26 species of epiphytic algae. While the abundance of Avicennia alba and Sonneratia sp were 459 ind/cm2 and 321 ind /cm2, in the coastal mangrove areas of Morodemak was found 28 species of epiphytic algae. The common epiphytic algae species found in all types of mangrove litter were Cymbella sp., Coscinodiscus sp., and Synedra ulna. In general, the group of Bacillariophyta (diatoms) is the dominant species in some mangrove leaf.

Co-Authors Amni Z. Rahman Anggi Aprilia Budi Prasetiani Bayu Pradika Bima Adistya Deni Zulfiana Febri Edo Fendy E. Wahyudi Insan Shiddiq Karyadi Baskoro Mochamad Hadi Mochammad Hadi Monica Sekar Rini Psn Masruri Sulistiyanto Ari Riche Hariyati Satwika Paramasatya, Satwika Sri Widodo Agung Suedy Sunarno Sunarno Udi Tarwotjo, Udi Widiartanto Widiartanto Zuni Miftakhurrohmah

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