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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Akademika Biologi Biosaintifika: Journal of Biology & Biology Education Jurnal Biologi Tropika NICHE Journal of Tropical Biology

Udi Tarwotjo, Udi

Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University

Author-ID : 517106

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

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Resistance Inheritance of Plutellaxylostella Population to Residual of Emamectin Benzoat Tarwotjo, Udi; Rahardian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8048

Excessive use of insecticides drives the increasing ability of pests to become resistant. The objectives of this research were to study the susceptibility and the resistance inheritance of the eleven population of P. xylostella to emamectin benzoate. The leaf-dip bioassay was applied to determine the sensitivity of P. xylostella to emamectin benzoate. The offspring of backcrossed F2 were tested whether the resistance was controlled by monogenic. The results showed that the LC50 of the Selo population was 53.42 ppb, and the Puasan population was 212.13 ppb. The genetic analysis showed that the backcrosseddegree of dominance (D) was less than 1. It was indicated that the P. xylostella resistance to emamectin benzoate was recessive. The value of LC50 of the backcrossed F1? x ?S (177.99 ppb) and its reciprocals x ?R (F1) (201.69 ppb) were not significantly different with the value of LC50 resistance population. This suggests that the nature of P. xylostella resistance to emamectin benzoate was controlled by monogenic.The result of the study would be beneficial for developing strategy to maintain susceptible population using refugee plant during lack of their host.

The Role of Acetylcholine Esterase in Resistance Mechanism of Plutella xylostella to Emamektin Benzoate Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13955

One of the resistance mechanism of P. xylostellato emamektin benzoate is target insensitivity which is acetylcholine esterase that responsible for resistance occurrence. The objective of this study was to determine the role of acetylcholinesterase in the resistance mechanism of P. xylostella population to emamektin benzoate. For enzyme activity analysis, larvae homogenate of the third instar of P. xylostella was prepared. The number of insects required for each scour is 1 for each field population. The protein content in P. xylostella homogenate was measured by the Folin-Ciocalteu test. Non-specific esterase activity with an absorption rate was read using ELISA reader tool with Î» = 450 nm.Â The inhibition level of acetylcholinesterase activity by emamectin benzoate in the tested population was 36.84%. The highest inhibition occurs in Puasan (Ngablak) population.Â The result shows that a Î±-naphthyl acetate substrate was used so that it was recorded as non-specific esterase activity and did not exhibit esterase activity which specifically describes emamectin benzoate. Non-specific esterase enzyme activity of either Î± or Î²-naphthyl acetate substances to benzoic emamectin in the tested population most of the population was still susceptible. On Î±-naphthyl acetate substrate, the highest absorbance value found in susceptible population to benzoate emamectin (0.773), while the lowest found in Babrik (Ngablak) populationÂ (0.083).

The Effectiveness of Plastic Mulch for Maintaining the Potato Farmland in Dieng Plateau Using Soil Biological Quality Index Agustina, Dian; Tarwotjo, Udi; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.17804

Dieng farmers apply plastic mulch to reduce erosion and to prevent the loss of nutrients. This study aimed to determine the effectiveness of plastic mulch in maintaining the quality of potato farmland using the Soil Biological Quality Index (QBS-ar) based on the existence of microarthropods. Soil sampling was conducted in six different locations, i.e., flat area with mulch, sloped area with mulch, the flat area without mulch, sloped area without mulch, flat forest area, and sloped forest area. Microarthropods were obtained from soil samples using a Berlese Funnel method. Soil quality was analyzed using QBS-ar index which was calculated based on Eco-morphological Index (EMI). The results showed that the value of QBS-ar index in potato farmland with the use of plastic mulch was much higher than in farmland without plastic mulch in bothflat and sloped area. Compared to the soil in the forest area, the value of QBS-ar index in farmland with the use of plastic mulch, especially in the flat area, was relatively similar. Hence, the use of plastic mulch for potato cultivation in the hilly area is effective in maintaining soil quality. This finding would be beneficial for developing a soil conservation strategy to achieve sustainable agriculture in Dieng agricultural area.

Beberapa Aspek Biologi Parasitoid Apantheles sp pada Inangnya, Spodopera litura, Fab. setelah Perlakuan Ekstrak Daun dan Ranting Aglaia odorata (Lour) Tarwotjo, Udi
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objectives of this study were to evaluate effect of leaf and branch extract of the Aglaia odorata against:Â emergences of adult Apantheles sp emerged from S. litura larvae, (2) the extract effect to live cycle and reproduction ofÂ Apantheles sp (3) and its effect against theÂ morphology character some of parasitoid. The metode was used by of leaf-dip method. The leaf and branch effectivity bioassay used by of Ricinus communis leaf . The leaf disk were dipped in the extract solution on six concentrationÂ for 10 s, and air dried. Each leaf disks was placedÂ six bottles glass and ten larvae were placed in each botlle, each concentration was replicated four time. Data collectedÂ were subjected an anaysis of variance followed by mean comparation based of DuncanÂ´t New Multiple Range Test. The imago Apantheles sp. emerged from host, S.Â litura larvae was recorded of live cycle, its reproduction, and morphology character. The result showed that the length of pre adult stage of emerging parasitoids from of S.Â litura larvae treated with 85,99 ppm was 45 percent, and with 21,95 ppm was 22,22 percent. The extract toxicity to natural enemies such as parasitoids relatively non toxis. The live cycle of female adult parasitoid was 16,90 and 6,20 days. Reproduction capacity of adult female parasitoids of 85,99 mg/L was 69,4 eggs/adult, which was statistically different from control, whereas of 21,95 treatment, it was 65,40 eggs/adult and its was not significant to control. Key words: Aglaia odorata, Lour, Spodoptera litura,, morphology character, Apanteles sp

Identifikasi Kandungan Senyawa Kimia Cuka Kayu dari Sekam Padi Nugrahaini, Dian Laila; Kusdiyantini, Endang; Tarwotjo, Udi; Prianto, A. Heru
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Wood vinegar rice husk contains a chemical compound that can be used as a biopesticides. Wood vinegar contained compounds which estimated potential as repellent, antifeedant, antimicrobials, antioxidants, disinfectants and as preservatives. The content of chemical compounds in the wood vinegar must be identified. Identification were done by calculating the concentration of acid, phenol and an infrared spectrophotometer. Production of wood vinegar made by the pyrolysis process used pirolisator. Most of the wood vinegar obtained is evaporated using a rotary evaporator and partly extracted using the method of separating funnel. Compound of acid and phenol in wood vinegar evaporation were higher than the extraction of wood vinegar. These results were confirmed by an infrared spectrophotometer spectrum showed the functional groups of acid and phenolic compounds.Â Keywords: wood vinegar rice husk, acid, phenol, pyrolysis, infrared spetrofotometer

Pengaruh Perbedaan Aplikasi Pestisida pada Struktur Komunitas Mikroarthropoda Tanah dalam Skala Lapangan dan Laboratorium Niswah, Rofiatun; Rahadian, Rully; Tarwotjo, Udi
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The choice of using pesticides with natural ingredients or synthetic chemicals is a differentiator between organic farming systems and inorganic farming systems. Pesticides are additives in agriculture to control pests or weeds. Abamectin is one of the active ingredients that can be found in pesticides. Ground microarthropods are members of the soil mesofauna, which are soil animals that have a body size ranging from 0.2-2 mm. The purpose of this study was to determine the effect of differences in the application of pesticides on a field or laboratory scale on the structure of soil microarthropod communities. Soil samples were taken from three agricultural lands, namely Bandungan inorganic agricultural land, Kopeng inorganic agricultural land and Kopeng organic agricultural land. The study was conducted in August to December 2018 in the Ecology and Biosystematic Laboratory of the Department of Biology, FSM Undip. Found a total of 60 species species richness originating from 13 orders. The application of pesticides which include the concentration and frequency of pesticide spraying has a negative influence on the structure of soil microarthopod communities. Application of abamectin pesticides on a laboratory scale proves that the administration of abamectin pesticides that exceeds the recommended concentration will adversely affect the structure of the soil microathropod community. Based on statistical tests of soil microarthropod species diversity from the results of the application of pesticides on a field and laboratory scale is significantly different.

Variasi Warna Dan Ketinggian Sticky Trap Dengan Atraktan Methyl Eugenol Sebagai Pengikat Serangga Polinator Dan Serangga Lainnya Pada Musim Bunga Pohon Jambu Air Merah Delima Tarwotjo, Udi; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this research was to determine the effectiveness of color and height variation of sticky trap with methyl eugenol on Insect visitting of flowers and other insect on apple water tree in Demak. Sampling of insects was conducting in one of apple water field in Demak using sticky trap with methyl eugenol attractant . There were three different colors of sticky trap and two height variation of trap placement were used. The data was analysed using descriptive analysis. Parameters observed included the number of fruit flies in each color, height of sticky trap, and the family of insects. The results showed that the insects found consist of 5 orders and 21 families. (Diptera, Hymenoptera, and Coleoptera, Lepidoptera, and Homoptera). . Most families are found in the order Diptera (8 families), Hymenoptera (6 families), Coleoptera (3 families), and Homoptera (4 families). The Diptera family consists of Tephritidae, Culicidae, Agromyzidae, Muscidae, Asillidae, Mycetophyllide, Drosophyllidae, Bombyllidae. The conclusion of this research is that the control of inseks visitting by using bright color sticky trap (yellow and white) with methyl eugenol attractant can be used as a trap of insect vistting of Diptera and other insects in the largest number of families

Struktur Komunitas Capung di Kawasan Wisata Curug Lawe Benowo Ungaran Barat Herlambang, Alamsyah Elang Nusa; Hadi, Mochamad; Tarwotjo, Udi
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Dragonflies have an important role for the stability of the ecosystem that is as predator and prey at the same time. The availability of food resources and optimal environmental conditions affect the species richness of dragonflies in the habitats. Reasearch on dragonfly comunity structure aims to find out the differences of community structure in each habitat type in the region of Curug Lawe Benowo. The research was conductet in 4 different stations which focus on species of dragonfly, amount of an individual species, habitats, environmental conditions, and the corellation between the variables. The method used is point count. The results showed that there are 19 dragonfly species which came from 7 different Family. The total number of individuals encountered from 4 stations is 205. The common species that can be found in all of the stations is Euphaea variegata. The level of diversity are medium, the level of evenness is fairly even. Similarity of species in any habitat types indicate that the habitats has a three kind of similarity levels that is fairly equal, less equal and not equal. Data analysis shows that there is a corellations between environmental conditions, and dragonfly species, affecting abundance and distributions of a dragonfly in the habitats, and can be used to describe dragonflies community structure in the region.Â Key words: Community structure, dragonfly, Curug Lawe-Benowo

Pengaruh Kandungan Ekstrak Daun Zodia (Evodia suaveolens) Terhadap Mortalitas Larva Nyamuk Aedes aegypti Basundari, Sinta Anas; Tarwotjo, Udi; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Aedes aegypti mosquito is a vector of dengue hemorrhagic fever (DHF). One place that is endemic by A.aegypti mosquitoes is the city of Semarang, specificallyÂ in Tembalang District. Tembalang district is one of the areas with highest DHF incidence rate. A.aegypti mosquito vector control is still using artificial insecticide, where the insecticide has negative impact to health and environment. Zodia leaves (Evodia suaveolens) are known to have potential as a natural insecticide, because they have secondary metabolites such as tannins, alkaloids, flavonoids that can be used as biolarvasides. This study aims to determine the content of phytochemical compounds of zodia leaves extract and to know the mortality in each population to zodia leaves extract. The extract of zodia leaf is obtained using maceration method. Chemical compounds contained in zodia leaves were analyzed using FT-IR. This research method is bioassay test, where the larvae are soaked with various series of concentration of zodia leaf extract. Each series of concentrations required ten larvae. The larvae used came from five kelurahan in Tembalang Subdistrict. The data were processed using probit analysis to obtain LC50. The results of LC50 obtained from the smallest to largest is 39,94 (34,67-45,28); 41,98 (36,6-48,07); 46,18 (39,28- 53,97); 47,62 (39,92-54,32) and 49,44 (42,24-58,25). The result of LC50 shows that is not significantly different, because there is the value of the overlapping fiducial limits between each population

Struktur Komunitas Odonata di Kawasan Wana Wisata Curug Semirang Kecamatan Ungaran Barat, Semarang Irawan, Frendi; Hadi, Mochamad; Tarwotjo, Udi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Dragonflies role as a predator in an ecosystem and become a healthy aquatic bio-indicators related to the life cycle. The diversity of habitat, food and predators affect community structure dragonfly. This study aims to determine the composition and community structure dragonfly morning and afternoon as well as the relationship with the physical environmental factors on four habitat in Semirang waterfall. Research using transect Point Count method. Statistically, the biodiversity of odonata in each habitat is significantly different. The results shows there are 15 species from 10 Family in morning observations and 12 species of 6 Family in afternoon observations, with a total of 17 species of 10 family of odonata. Biodiversity in each station is low to moderately with high evenness. The highest abundance found in river habitat without the canopy during the afternoon that Vestalis luctuosa (61.29%), while the lowest abundance found in the the canopied river habitat when afternoon that is Drepanosticta spatulifera (1.33%). Common species at area of Semirang waterfall are Euphaea variegata, Orthetrum sabina and Vestalis luctuosa. Endemic species at area of Semirang waterfall are Heliocypha fenestrata, Drepanosticta spatulifera and D. sundana.Â Key words : Community structure, Odonata, Semirang waterfall

Co-Authors Adhi, Siska Lesiana Alamsyah Elang Nusa Herlambang, Alamsyah Elang Nusa Basundari, Sinta Anas Deni Elisabeth Dian Agustina Dwiyani Anjar Martitik Edhi Martono Edhy Martono, Edhy Endang Kusdiyantini Febri Edo Insan Shiddiq Irawan, Frendi Jafron Wasiq Hidayat Jesmandt Situmorang Mochamad Hadi Mubarok, Rizal Husni Murtiati, Sri Nasik, Isman Niswah, Rofiatun Noora Lailatul Husna Nugrahaini, Dian Laila Prianto, A. Heru Psn Masruri Sulistiyanto Ari R.C. Hidayat Soesilohadi Rully Rahadian Rully Rahadian Rully Rahadian Rully Rahardian, Rully Sari, Vicka Kusuma Wiatri Larasati

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