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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Puji Sulistiana
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Perbanyakan In Vitro Sansevieria trifasciata ‘Lorentii’: Regenerasi Tunas, Pengakaran, dan Aklimatisasi Planlet Yusnita, ,; Wahyuningsih, Triani; Sulistiana, Puji; Hapsoro, Dwi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.441 KB) | DOI: 10.24831/jai.v41i1.7080

Abstract

This research aimed to study effects of benzyladenine (BA) on in vitro shoot formation and effects of indolebutyric acid(IBA) and acclimatization media on ex vitro rooting and acclimatization of Sansevieria trifasciata ‘Lorentii’. Leaf segmentswere taken from young fully-expanded leaves, surface sterilized and cultured on Murashige and Skoog (MS) basal mediumcontaining 0.25 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) for 2 weeks, transferred to medium without growth regulatorfor 2 weeks, and then subcultured on MS medium containing BA (0, 0.5, 1 and 2 and 5 mg L-1). The results showed thatadventitious shoot regeneration occured after callus formation. The best BA concentration was 2 mg L-1, producing 4.5 shootsper explants in 3 months and 11.1 shoots per explant in 4 months. Application of 2000 ppm IBA and the use of acclimatization  medium consisting rice husk charcoal and compost (1:1) produced the highest number of primary roots, length of roots and root fresh weight. However, the ex vitro rooting did not influence the success of plantlet acclimatization, the survival rate being 96% and there were no significant difference in plant growth.Keywords: benzyladenine, ex vitro, indolebutyric acid, 2,4-dichlorophenoxyacetic acid, micropropagation, Sansevieriatrifasciata
Co-Authors , Yusnita DWI HAPSORO Triani Wahyuningsih