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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology REINWARDTIA BERITA BIOLOGI Makara Journal of Science Reinwardtia
Lulut Dwi Sulistyaningsih
Herbarium Bogoriense
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Veterinary

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Preface Volume 23 (2) Year 2024 Sulistyaningsih, Lulut Dwi
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

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Front Cover, Reinwardtia Volume 23 No 2 (2024) Sulistyaningsih, Lulut Dwi
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

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Front cover Reinwardtia 23 (2) 2024
ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE SUHARSONO, SUHARSONO; TRISNANINGRUM, NIKEN; SULISTYANINGSIH, LULUT DWI; WIDYASTUTI, UTUT
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.362 KB) | DOI: 10.11598/btb.2009.16.1.64

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Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.   Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine
PHYLOGENETICAL STUDY OF WILD BANANA SPECIES (Musa L.) IN SULAWESI INFERRED FROM INTERNAL TRANSCRIBED SPACER REGION OF NUCLEAR RIBOSOMAL DNA SEQUENCES Sulistyaningsih, Lulut Dwi; Megia, Rita; Widjaja, Elizabeth Anita
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15465.995 KB) | DOI: 10.11598/btb.2014.21.1.311

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Study to determine the phylogenetic relationship of wild banana species (Musa) in Sulawesi based on the ITS regions sequences has been done. A total of 28 samples including 16 ITS sequences from GeneBank were used. Species of Ensete and Musella were incorporated as an outgroup. Total DNA was extracted by the modified CTAB. ITS-5 and ITS-4 primers were used to amplify the ITS regions. Data sequences were edited by ChromasPro and were aligned by Muscle software.Some unique nucleotides were found in M. balbisiana, M. itinerans, and M. textilis sequences. Strict consensus tree revealed from Maximum Parsimony (MP) as well as Bayesian analysis showed Musa textilis was separated from other taxa in which M. textilis become as the basal sister to M. balbisiana. By using MP analysis, M. itinerans together with M. acuminata ssp. banksii, M. acuminata var. tomentosa, and M. celebica placed in the same clade, whereas Bayesian analysis showed M. itinerans was separated and placed in different clade.
Calamus vitiensis (ARECACEAE), A NEW RECORD OF RATTAN IN SUMBAWA ISLAND, INDONESIA Rustiami, Himmah; Sulistyaningsih, Lulut Dwi
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.1.1012

Abstract

Calamus vitiensis, previously known to occur only in Papua New Guinea, Australia (Queensland), the Solomon Islands, Vanuatu, and Fiji is now also found in the mountainous area of Batudulang, West Sumbawa, Lesser Sunda Islands, Indonesia. This paper presents a complete report of the description, notes, and illustrations of the species.
Co-Authors Deby Arifiani, Deby Elizabeth Anita Widjaja Esthi, Yunita Nur Himmah Rustiami, Himmah J. F. VELDKAMP, J. F. MARLINA ARDIYANI, MARLINA RITA MEGIA Rugayah Rugayah Sahroni, Deni Suharsono Suharsono Trisnaningrum, Niken UTUT WIDYASTUTI