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Isolation, serotyping, antimicrobial minimum inhibitory concentration and phatogeneticity determination of erysipelothrixrhusiopathiae from tonsils of apparently healthy slaughter pigs. Kamaluddin Zarkasie; Toshio Takahashi; Siti Mariana; Sumadi .
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

Erysipolothri rhusiopathiae was isolated from tonsils of 245 (37.5%) of 687 appparently healthy slaughter pigs. Serotyping was performed by an agar gel double diffusion test. Of these 245 isoates, 95 (38.8%) were serotype N, 58 (23.7%) serotype 2, 18 (7.3%) serotype 11, 13 (5.3%) serotype 12, and the remaining 61 isolates were sterotypes 1a, 3, 4, 5, 6, 8, 9, 10, 13, 19 and 22. Antimicrobial Minimum Inhibitiory Concentration (MIC) was determined by two-fold agar dilution test. All isolates were highly suspectible to penicillin G, ampicillin and crythromycin and moderately susceptible to olean domycin, oxytetracyline, chloramphenicol and dihydrostreptomycin and moderately suspectible to oleandomicin, oxytetracyline, chloramphenicol and dihydrostreptomycin. Sulfadimethoxine showed no activity against the isolates. Pathogenicity test showed highly virulent for mica (LD5 0 < 10 3-0 CFU) whereas serotypes 19 and 22 were non-virulent.
Reproductive performances of Boe, Kacang and Boerka does Simon Elieser; Sumadi .; G Suparta; Subandriyo .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.639 KB) | DOI: 10.14334/jitv.v17i2.683

Abstract

Does reproduction performance could be expressed by her ability to give birth and to give milk to their kids during the pre-weaning period. This study was aimed to evaluate the reproductive performance of Boer, Kacang and Boerka does; and was carried out for two years at Research Institute for Goat Production, Sungei Putih. The materials used were goats owned by the institute. The parameters observed were: litter size, parity of does, preweaning mortality, kidding interval and sex ratio of kids. The rate of reproduction of the does was estimated using Amir and Knipscheer methods and were statistically analyzed using General linear model. Results showed that litter size and kidding interval of Boer goats were higher (P < 0.05) then that of Kacang goats, while the Boerka goats was in between. The mortality at preweaning of Boer goats was lower (P < 0.05) then that of Kacang goats, while the Boerka goats was in between. The percentage of kid sex ratio of three breeds were fluctuated. Parity of does had significant effect on all reproduction traits (P < 0.05) except for sex ratio of kids. The does reproduction was smallest at the first parity, and increased with the increase of parity from one to four, and then decreased in subsequent parities. The highest does reproduction rate was found in the Boerka (1.82), followed by Boer (1.80) and Kacang (1.80). It is concluded that the reproductive performance of the three breed female goat was relatively the same. Key Words: Performance, Reproductive, Boer, Kacang
Morphological diversity and genetic differentiation of PO cattle in smallholder farmers Hartati .; Sumadi .; Subandriyo .; Tety Hartatik
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.518 KB) | DOI: 10.14334/jitv.v15i1.680

Abstract

PO cattle is one of  the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer  based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008.  PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of  alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower  compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed  high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci  indicating the increasing of inbreeding in  the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle
Follicle development and FSH secretion pattern of Ongole crossbred cow with natural twin birth history Aryogi .; Baliarti E; Sumadi .; Kustono .
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.486 KB) | DOI: 10.14334/jitv.v18i3.318

Abstract

A research was done to obtain basic data about influence of natural twin birth history on reproduction of Ongole crossbred (PO) cow, as a basic consideration in utilizing twin genetic potency to increase beef production. The research was done for three estrus cycles respectively, and consists of two activities. The first activity was done in Beef Cattle Research Station (BCReS) at Grati sub-district, Pasuruan district – East Java, to observe number and development of follicle of ten cows with natural twin birth history (TP) and ten cows with single birth history (SP). In this activit ultra sonography (USG) equipment was used. The second activity was done in BCReS and in the Faculty of Veterinary Airlangga University at Surabaya, to observe concentration and profile of FSH using Bovine Blood Serum kit and IRMA method of five cows with TP and five PO cows with SP. Data obtained were analyzed using Chi-square test, t-test and descriptive presentation. Result shows that 23.33% of cows with TP produced two dominant (de Graf) follicles in an estrus cycle and significantly (P < 0.01) higher FSH secretion concentration (1.26 – 3.13 times) than that of cows with SP. It is concluded that PO cows with TP can produces more than one de Graf follicles in an estrus cycle and it has very high secretion concentration level of FSH. Key Words: Twin Births, PO Cow, Follicle, FSH
PENGARUH BOKASHI KOTORAN SAPI DAN PUPUK FOSFAT TERHADAP MUTU FISIOLOGIS BENIH KEDELAI (Glycine max L. Merill) KULTIVAR GROBOGAN Wulandari M M; Sumadi .; M Kadapi
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v9i1.5047

Abstract

The most important increasing productivity soybean in physiological quality of seed. There for, soybean cultivation also improving soil quality for increase yield and seed quality. This research was aimed to obtain the best combination doses of bokashi cattle manure and phosphate fertilizer on yield and seed quality. This research was held at Faculty of Agriculture Padjadjaran University experimental garden in Sumedang regency and Seed Technology Labotary from November 2016 to Februari 2017. The design that used in this research was Randomized Completely Block Design and Scott-Knott at 5% rate. Treatment used in this research were Grobogan cultivars with tested combinations doses bokashi 0 t ha-1, 16 t ha-1, 32 t ha-1 and 48 t ha-1, each combined with (SP-18) doses 0 kg ha-1, 16 kg ha-1, 32 kg ha-1 and 48 kg ha-1 which were repeated three times. The result of experiment indicated significantly for weight 100 grains and quality of seed. This experiment suggested that dosage of 48ton/ha bokashi cattle manure and 32 kg ha-1 phosphate fertilizer in the best combinations of soybean crop.Keywords: Bokashi, Phosphate fertilizer, Physiological quality, Soybean seed
SISTEM MONITORING STATUS LAMPU PENERANGAN JALAN UMUM BERBASIS SENSOR JARINGAN NIRKABEL (WIRELESS SENSOR NETWORK) mayda waruni kasrani; sumadi .
Jurnal Teknik Elektro Uniba (JTE UNIBA) Vol. 1 No. 2 (2017): Jurnal Teknik Elektro Uniba (JTE UNIBA)
Publisher : Lembaga Penelitian Universitas Balikpapan

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Abstract

Abstract— The wireless sensor network system is applied to themonitoring system of street light. There are several parameter thatmonitored such as electric current, voltage, GPS map coordinate,Lux, and status off on and of of the light. The node sensor thatcontain of Arduino Mega 2560, ACS712ELCTR-20A-T currentsensor, ZMPT101B voltage sensor, TSL2561 light sensor, GPSNEO6MV2 module, TFT display and SIM900A GSM module. GSMModule SIM900A is as a communication tool between sensor nodedevices with web monitoring application.The monitoring system of street light parameter status isdesign to sned the reading parameter data such as electrical current,voltage, Lux and GPS coordinate map and then send to the web database on the web server. The data that recorded on the web serverthan can be view through the web application without locationrestriction as long as internet connection is available. Theapplication also can control to turn on and off the light. Intisari— Jaringan sensor nirkabel yang diaplikasikan pada sistemmonitoring berupa parameter cahaya, arus listrik, tegangan listrik,peta koordinat dan indikasi status hidup dan mati lampu. Perangkatyang digunakan dalam sistem ini adalah titik sensor (sensor node)danaplikasi pengawasan dengan web (web monitoring). Rancangansensor node menggunakan perangkat berupa Arduino Mega 2560,sensor arus ACS712ELCTR-20A-T, sensor tegangan ZMPT101B,sensor cahaya TSL2561, modul GPSNEO 6MV2, TFT display danmodul GSM SIM900A. Modul GSM SIM900A digunakan sebagaialat komunikasi antara perangkat sensor node dengan aplikasi webmonitoring.Sistem monitoring status lampu penerangan jalan umum yangdirancang mampu melalukan pembacaan parameter arus listrik,tegangan listrik, intensitas cahaya (Lux), dan peta koordinat GPS.Data parameter yang dibaca selanjutnya dikirim ke dalam basis datadi web server melalui jaringan komunikasi GSM. Data yang telahdirekam di dalam web server tersebut dapat dilihat pada sebuahaplikasi web sehingga user dapat memonitor dengan mudah tanpabatasan lokasi selama terdapat koneksi internet. Aplikasi web yangdibangun juga dirancang mampu mengontrol hidup dan mati titiklampu yang dimonitor.