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All Journal Hemera Zoa Microbiology Indonesia
Kamaluddin Zarkasie
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Isolation, serotyping, antimicrobial minimum inhibitory concentration and phatogeneticity determination of erysipelothrixrhusiopathiae from tonsils of apparently healthy slaughter pigs. Kamaluddin Zarkasie; Toshio Takahashi; Siti Mariana; Sumadi .
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Erysipolothri rhusiopathiae was isolated from tonsils of 245 (37.5%) of 687 appparently healthy slaughter pigs. Serotyping was performed by an agar gel double diffusion test. Of these 245 isoates, 95 (38.8%) were serotype N, 58 (23.7%) serotype 2, 18 (7.3%) serotype 11, 13 (5.3%) serotype 12, and the remaining 61 isolates were sterotypes 1a, 3, 4, 5, 6, 8, 9, 10, 13, 19 and 22. Antimicrobial Minimum Inhibitiory Concentration (MIC) was determined by two-fold agar dilution test. All isolates were highly suspectible to penicillin G, ampicillin and crythromycin and moderately susceptible to olean domycin, oxytetracyline, chloramphenicol and dihydrostreptomycin and moderately suspectible to oleandomicin, oxytetracyline, chloramphenicol and dihydrostreptomycin. Sulfadimethoxine showed no activity against the isolates. Pathogenicity test showed highly virulent for mica (LD5 0 < 10 3-0 CFU) whereas serotypes 19 and 22 were non-virulent.
Development of Rapid Agglutination Test to Detect Chicken Marek Antibody I WAYAN TEGUH WIBAWAN; LIA SITI HALIMAH; TITIEK DJANNATUN; KAMALUDDIN ZARKASIE
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.112 KB) | DOI: 10.5454/mi.3.2.5

Abstract

To our knowlege, there is no rapid agglutination test to detect antibodies to viruses which might be due to the small dimension of viral particles. Through complex formation of Staphylococcus aureus bearing protein A-rabbit IgG-anti IgY-IgY anti Marek virus, agglutination of antibodies to viruses can be achieved and visualized. To design the prototype of the test, the bacterial cells of Staphylococcus aureus were coupled to a complex compound consisting of IgG-IgY-Marek antigen. This protocol was able to detect clearly the presence of Marek antibody in chicken sera, showing the rapid, clear and distinct agglutination reaction on the glass objects. No agglutination reaction was observed in the reaction of specified pathogen free chicken sera with this prototype showing the specificity of the test. This finding demonstrates a novel rapid agglutination which can be used for the detection of antibodies to various agents.
Co-Authors I wayan Teguh Wibawan LIA SITI HALIMAH Siti Mariana Sumadi . Titiek Djannatun Toshio Takahashi