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All Journal Hemera Zoa Microbiology Indonesia The Indonesian Biomedical Journal
Kamaluddin Zarkasie
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Isolation, serotyping, antimicrobial minimum inhibitory concentration and phatogeneticity determination of erysipelothrixrhusiopathiae from tonsils of apparently healthy slaughter pigs. Kamaluddin Zarkasie; Toshio Takahashi; Siti Mariana; Sumadi .
Hemera Zoa Vol. 74 No. 1 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Erysipolothri rhusiopathiae was isolated from tonsils of 245 (37.5%) of 687 appparently healthy slaughter pigs. Serotyping was performed by an agar gel double diffusion test. Of these 245 isoates, 95 (38.8%) were serotype N, 58 (23.7%) serotype 2, 18 (7.3%) serotype 11, 13 (5.3%) serotype 12, and the remaining 61 isolates were sterotypes 1a, 3, 4, 5, 6, 8, 9, 10, 13, 19 and 22. Antimicrobial Minimum Inhibitiory Concentration (MIC) was determined by two-fold agar dilution test. All isolates were highly suspectible to penicillin G, ampicillin and crythromycin and moderately susceptible to olean domycin, oxytetracyline, chloramphenicol and dihydrostreptomycin and moderately suspectible to oleandomicin, oxytetracyline, chloramphenicol and dihydrostreptomycin. Sulfadimethoxine showed no activity against the isolates. Pathogenicity test showed highly virulent for mica (LD5 0 < 10 3-0 CFU) whereas serotypes 19 and 22 were non-virulent.
Development of Rapid Agglutination Test to Detect Chicken Marek Antibody I WAYAN TEGUH WIBAWAN; LIA SITI HALIMAH; TITIEK DJANNATUN; KAMALUDDIN ZARKASIE
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.112 KB) | DOI: 10.5454/mi.3.2.5

Abstract

To our knowlege, there is no rapid agglutination test to detect antibodies to viruses which might be due to the small dimension of viral particles. Through complex formation of Staphylococcus aureus bearing protein A-rabbit IgG-anti IgY-IgY anti Marek virus, agglutination of antibodies to viruses can be achieved and visualized. To design the prototype of the test, the bacterial cells of Staphylococcus aureus were coupled to a complex compound consisting of IgG-IgY-Marek antigen. This protocol was able to detect clearly the presence of Marek antibody in chicken sera, showing the rapid, clear and distinct agglutination reaction on the glass objects. No agglutination reaction was observed in the reaction of specified pathogen free chicken sera with this prototype showing the specificity of the test. This finding demonstrates a novel rapid agglutination which can be used for the detection of antibodies to various agents.
High-yield and Functional IgY Anti-S1 SARS-CoV-2 Delta Variant from Chicken Immunized with In-house Recombinant Protein Zarkasie, Kamaluddin; Ginting, Teridah Ernala; Poeloengan, Andrea Hynan; Andriani, Febi; Lages, Aksar Chair; Cornelia, Vina; Nugraha, Anggah; Rosidi, Bustanur; Indrasari, Setyarina; Nidom, Chairul Anwar; Yusuf, Irawan
The Indonesian Biomedical Journal Vol 17, No 4 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i4.3693

Abstract

BACKGROUND: During COVID-19 pandemic, effective preventive and therapeutic strategies was urgently necessary, especially with the emerging Delta variants. Previous existing interventions have some limitations, and passive immunization using immunoglobulin Y (IgY) is considered viable. However, the potential of IgY antibodies remains underexplored locally. Therefore, a feasible study was conducted to produce IgY against the SARS-CoV-2 Delta variant from chickens raised on a farm, instead of a laboratory environment, by using in-house recombinant protein, to offer a promising low-cost alternative for passive immunization.METHODS: In-house HEK293 cell line was produced by secreting recombinant S1 subunit protein of SARS-CoV-2 Delta variant. The protein was confirmed by western blot and used as an immunogen in two chickens. IgY was extracted from egg yolks. Titers and neutralization activity of IgY against SARS-CoV-2 Delta variant were confirmed by enzyme-linked immunosorbent assay (ELISA) and surrogate virus neutralization test (sVNT). The functionality of IgY was then tested on lysates from COVID-19 patients' nasopharyngeal swabs with dot blot method.RESULTS: The high anti-S1 IgY titers and neutralization activity was confirmed following immunization with 1 mg immunogen at 2–3-week intervals. IgY titers varied between chickens and time points. The mean titers showed significant increase after the fifth immunization (1.21, 1.72 and 1.48; with p<0.05 for all). Neutralization activity appeared after the second immunization and was significant after the third immunization (31.9%, 34.95%, 26.9%, 47.6%, 54.95% and 57.3%; with p<0.05 for all). The results of this study showed that the extracted IgY reacted to COVID-19 patients' nasopharyngeal swabs lysates.CONCLUSION: A high-yield and functional IgY anti-S1 SARS-CoV-2 Delta variant can be produced from chicken immunized with an in-house recombinant protein. This can be an alternative for affordable and effective IgY production during public health emergencies.KEYWORDS: IgY, SARS-CoV-2, S1 subunit protein, egg yolk, chicken
Co-Authors Aksar Chair Lages, Aksar Chair Andriani, Febi Cornelia, Vina Ginting, Teridah Ernala I wayan Teguh Wibawan Indrasari, Setyarina Irawan Yusuf LIA SITI HALIMAH Nidom, Chairul Anwar Nugraha, Anggah Poeloengan, Andrea Hynan Rosidi, Bustanur Siti Mariana Sumadi . Titiek Djannatun Toshio Takahashi