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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Akuatika BIOEDUSCIENCE Tropical Animal Science Journal Jurnal Kebijakan Perikanan Indonesia Pharmacoscript Makara Journal of Science Menawan : Jurnal Riset dan Publikasi Ilmu Ekonomi International Journal of Educational Evaluation and Policy Analysis
Yasman Yasman
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Library & Information Science Mathematics Medicine & Pharmacology Public Health Social Sciences

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Journal : Pharmacoscript

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QUANTIFICATION OF TOTAL FLAVONOID CONTENT IN FRACTIONATED YOUNG LEAF EXTRACTS OF RED SHOOT LEAVES (Syzygium myrtifolium) USING UV-VIS SPECTROPHOTOMETRY Vilya, Syafriana; Rika, Amelia; Nissa, Maftucha; Munawarohthus, Sholikha; Windri, Handayani; Yasman, Yasman
Pharmacoscript Vol. 8 No. 1 (2025): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v8i1.2075

Abstract

Syzygium myrtifolium (red shoot leaves) is widely known for its rich phytochemical profile, particularly flavonoids, which exhibit various pharmacological properties. This study aimed to determine the total flavonoid content in the crude extract, n-hexane fraction, ethyl acetate fraction, and water fraction of red shoot leaves (Syzygium myrtifolium) using a colorimetric method with quercetin as a standard. The results revealed that the ethyl acetate fraction had the highest flavonoid content (65.781 ± 6.365 mgQE/g), followed by the crude extract (26.093 ± 0.961 mgQE/g), n-hexane fraction (18.293 ± 2.925 mgQE/g), and water fraction (12.583 ± 0.824 mgQE/g). The study also found that the solvent polarity significantly influenced the flavonoid extraction efficiency, with ethyl acetate, a moderately polar solvent, being most effective for isolating flavonoid aglycones. The n-hexane fraction contained polymethyl flavonoids, which are more soluble in nonpolar solvents, while flavonoid glycosides were more soluble in polar solvents like water and alcohol mixtures. Furthermore, the research highlights the importance of extraction methods, as the total flavonoid content in mature red shoot leaves was found to be higher than in younger leaves, likely due to increased secondary metabolite production in mature tissues. This study emphasizes the role of solvent polarity in flavonoid extraction and provides insights into the phytochemical composition of S. myrtifolium leaves for potential applications in natural product research.
QUANTIFICATION OF TOTAL FLAVONOID CONTENT IN FRACTIONATED YOUNG LEAF EXTRACTS OF RED SHOOT LEAVES (Syzygium myrtifolium) USING UV-VIS SPECTROPHOTOMETRY Vilya, Syafriana; Rika, Amelia; Nissa, Maftucha; Munawarohthus, Sholikha; Windri, Handayani; Yasman, Yasman
Pharmacoscript Vol. 8 No. 1 (2025): Pharmacoscript
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/pharmacoscript.v8i1.2075

Abstract

Syzygium myrtifolium (red shoot leaves) is widely known for its rich phytochemical profile, particularly flavonoids, which exhibit various pharmacological properties. This study aimed to determine the total flavonoid content in the crude extract, n-hexane fraction, ethyl acetate fraction, and water fraction of red shoot leaves (Syzygium myrtifolium) using a colorimetric method with quercetin as a standard. The results revealed that the ethyl acetate fraction had the highest flavonoid content (65.781 ± 6.365 mgQE/g), followed by the crude extract (26.093 ± 0.961 mgQE/g), n-hexane fraction (18.293 ± 2.925 mgQE/g), and water fraction (12.583 ± 0.824 mgQE/g). The study also found that the solvent polarity significantly influenced the flavonoid extraction efficiency, with ethyl acetate, a moderately polar solvent, being most effective for isolating flavonoid aglycones. The n-hexane fraction contained polymethyl flavonoids, which are more soluble in nonpolar solvents, while flavonoid glycosides were more soluble in polar solvents like water and alcohol mixtures. Furthermore, the research highlights the importance of extraction methods, as the total flavonoid content in mature red shoot leaves was found to be higher than in younger leaves, likely due to increased secondary metabolite production in mature tissues. This study emphasizes the role of solvent polarity in flavonoid extraction and provides insights into the phytochemical composition of S. myrtifolium leaves for potential applications in natural product research.
Co-Authors A. Sofyan A. Sofyan Abinawanto Abinawanto Aini, Fadita Nurul Arum Albuntana Cham, Mbara Ediviani, Wanda Fachrul Razi H. Julendra Handayani, Windri Hernuryadin, Yayan L. Istiqomah Laksmana, Bayu Arif Pasa Limgiani Limgiani M. F. Karimy M. F. Karimy Maryenti, Tety Munawarohthus, Sholikha Nisa, Upi Chairun Nissa, Maftucha Octavia, Silvi Riani Widiarti Rika, Amelia Syafriana, Vilya Windri, Handayani Wisnu Wardhana