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All Journal Jurnal Ilmu Nutrisi dan Teknologi Pakan
S Suryahadi
Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry

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Karakteristik Fermentasi Rumen In vitro dengan Penambahan Sabun Kalsium Minyak Nabati pada Buffer yang Berbeda S Suharti; D N Aliyah; S Suryahadi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 16 No. 3 (2018): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.975 KB) | DOI: 10.29244/jintp.16.3.56-64

Abstract

Canola oil and flaxseed oil are vegetable oils as potential sources of unsaturated fatty acid that could improve the production and quality of beef meat. However, the use of vegetable oils need to be protected to avoid biohydrogenation by rumen bacteria. The research was aimed to analyse effect of flaxseed/canola oils calcium soap in the different buffer media on in vitro fermentation characteristic. The experiment was conducted in a factorial randomized block design with 2 factors and 3 blocks based on rumen sampling time. The first factor was sources of vegetable oils (canola and falxseed) and the second factor was type of buffer (Kajikawa and McDougall). Variables observed were pH value, N-NH3 concentration, total volatile fatty acid (VFA), dry matter and organic matter digestibility. Data obtained was analysed using Analysis of Variance and any significant different further tested using Duncan Multiple Range Test. The results showed that there was no interaction between sources of diet and buffer. Supplementation of canola and flaxseed oils protected by calcium soap at level 6% did not affect pH value, dry matter digestibility, rumen protozoa and total bacteria. The use of different buffers affected pH value, dry matter digestibility, rumen protozoa and total bacteria. It’s concluded that the used of flaxseed oil or canola oil calcium soap did not alter rumen fermentation and McDougall buffer could improve in vitro fermentation activity compare to Kajikawa buffer. Keywords: biohydrogenation, calcium soap, canola oil, flaxseed oil, rumen fermentation
Impact of Inoculant Sources on Feed in Vitro Fermentability and Digestibility : Dampak Cairan Rumen Sumber Inokulan pada Fermentabilitas dan Kecernaan Pakan secara In Vitro A K Agustina A K Agustina; D Evvyernie; Rika Zahera; I G Permana; Toto Toharmat; S Suryahadi; D Despal
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 18 No. 3 (2020): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.18.3.89-94

Abstract

The aim of this research is to compare alternative inoculant source for in vitro rumen fermentation. In the first experiment, inoculant from fistulated cattle kept in LIPI and IPB (Fis1 and Fis2) and inoculant from Bogor municipality abattoir and IPB abattoir (Abo1 and Abo2) were tested for their pH, total bacterial count, and protozoal number using a complete block design with four replications. In the second experiment, the effect of the inoculant sources was tested on cornmeal (F1), soybean oil meal (F2), Napier grass (F3), and dairy cattle complete ration (F4) fermentability and digestibility including pH, VFA, NH3, IVDMD and IVOMD parameters. The results showed an unsignificant different protozoal number among inoculant sources. The pH of Fis2 rumen liquor was significantly lower (p<0.05) than others. The bacterial population was significantly higher (p<0.05) in Fis2 and Abo2 than Abo1, and Fis1. The inoculant pH after feed fermentability was not influenced by feed type but inoculant source with Fis1 was significantly higher (p<0.05) than Fis2, Abo2, and Abo1. The ammonia, VFA concentration, IVDMD, and IVOMD were influenced by interaction between inoculant sources and feed types. Although inoculant from cattle close to the laboratory (Fis2 and Abo2) were better in term of higher bacterial population, higher fermentability and digestibility for most type of feeds but other sources can be used in vitro study without differences in average fermentability and digestibility results. Key words: abattoir, fermentability, fistula, inoculant, in vitro
Co-Authors A K Agustina A K Agustina D N Aliyah Despal Despal Dwierra Evvyernie I G Permana Rika Zahera S Suharti Toto Toharmat