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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Tropical Animal Science Journal Buletin Ilmu Makanan Ternak Jurnal Ilmu Nutrisi dan Teknologi Pakan Jurnal Ilmu Peternakan Terapan Agrinimal Jurnal Ilmu Ternak dan Tanaman

Dwierra Evvyernie

Bogor Agricultural University

Author-ID : 261353

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Immunology & microbiology Veterinary

Published : 31 Documents Claim Missing Document

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Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage Prihantoro, Iwan; Toharmat, T; Evvyernie, D; ., Suryani; Abdullah, L
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Local buffalo rumen fluid is a source of fiber digesting bacteria. Such bacteria presumably are well adapted to feed stuffs derived from agricultural byproducts with low quality. The purposes of this study were: (1) to isolate the fiber-digesting bacteria from buffalo rumen fluid, (2) to determine the fiber digesting characteristics and adaptability of such bacteria, and (3) to characterize the bacterial diversity.Â Rumen fluids for the experiment were collected from buffaloes slaughtered at the Faculty of Animal Science, Bogor Agricultural University abattoir. Fiber-digesting bacteria were isolated using McDougall buffer supplemented with fibrous feed. A factorial experiment was conducted to study characteristics and adaptability of the bacteria using two factors: the type of bacteria and type of feed source of the fiber. Diversity among the bacteria was analyzed using the NTSys2.10 program.Results showed that nine bacterial isolates had a high adaptability to fiber feed based on CMCase. The highest CMCase activity bacteria for Pennisetum purpureum were A9 (11.36Â±1.70 unit/ml/h), A3 (11.22Â±0.60 unit/ml/h) and A42 (10.62Â±1.96 unit/ml/h). CMCase activity of fiber digesting bacteria from buffalo rumen fluid was not correlated with the number of bacteria in the culture. Based on genetic similarity, nine isolates were grouped into five types having similarityâ¥46%. Key Words: Buffalo Rumen Fluid, Fiber-Digesting Bacteria, Low Quality Forage

Potency of fiber rumen bacterial isolates from local buffalo inoculated into Frisian Holstein calves during preweaning period Prihantoro, Iwan; Evvyernie, D; ., Suryani; Abdullah, L; Yunitasari, N.S; Sari, A.P; Khairunisa, D; Haziq, A; Rahayu, N; Toharmat, T
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Fiber-digesting bacteria are the main rumen bacteria that play an important role in digesting feed. These bacteria are adapted to low quality forage from agricultural byproduct. The aim of these study was to determine the potency of fiber-digesting bacteria consortium obtained from buffalo rumen inoculated to Frisian Holstein calves during preweaning on feed consumption, utilization, mineral uptake and physiological status. This study used 14 isolates of bacteria obtained from collection of Faculty of Animal Science, Bogor Agricultural University. The experimental unit consisted of six Frisian Holstein calves at two week old with the average body weight of 38.00 Â± 6.23 kg. Calves were inoculated by 20 ml of fiber-digesting rumen bacterial isolates [4.56 x 109 cfu/ml] every morning for four weeks. Experimental design used was based on a completly randomized design with three calves received the respective inoculation (treatment group) and three calves without any inoculation (control group). Data were analyzed statistically using t-test method with Î± = 0.05 and 0.01. The results showed that fiber-digesting bacteria (FDB) from rumen buffalo have adapted in the calves rumen since preweaning periode. Inoculation FDB increased the number of rumen bacteria, digestibility of protein and P uptake calves at eight weeks old. Increased feed intake, uptake of Mg and cobalt calves at 14 weeks old. Without causing any negative effects on ADG, physiological status and rumen fermentability. Key Words: Fiber-Digesting Bacteria, Nutrient Intake, Rumen Fermentability

Penggunaan Kromium Organik dari Beberapa Jenis Fungi terhadap Aktivitas Fermentasi Rumen Secara in Vitro W D Astuti; T Sutardi; D Evvyernie; T Toharmat
Media Peternakan Vol. 29 No. 3 (2006): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Chromium appears to be an essential trace element since 1959, but its effect on ruminal microbes is not clear yet. This experiment was conducted to study the effects of organic chromium supplementation on rumen fermentation activity. An in vitro technique was held using randomized block design with 13 treatments and 3 replications. There were four kinds of organic Cr used, produced with four different species of fungi as carriers. Fungi used as carriers were Saccharomyces cerevisiae, Aspergillus oryzae, Rhizophus oryzae and "ragi tape". The result indicated that the optimum organic Cr supplementation was 1 mg organic Cr/kg dry matter. Supplementation of 1 mg organic Cr/kg dry matter increased dry matter and organic matter digestibilities. It also tended to increase NH3 and total VFA production. Propionate production increased, which decreased methane production and increased hexose conversion efficiency in several treatments. Each fungus used as carrier of organic Cr resulted in different effects on rumen fermentation activity, but the effects was within a normal range. It was concluded that either Saccharomyces cerevisiae, Aspergillus oryzae, Rhizophus oryzae or "ragi tape" could be used as carrier in organic Cr production. Key words : organic Cr, ruminal microbes, Saccharomyces cerevisiae, Aspergillus oryzae, Rhizophus oryzae, "ragi tape"

Inkorporasi Kromium pada Khamir dan Kapang dengan Substrat Dasar Singkong yang Diberi Kromium Anorganik W D Astuti; T Sutardi; D Evvyernie; T Toharmat
Media Peternakan Vol. 29 No. 2 (2006): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Organic-chromium (Cr) has higher availability for animals than inorganic-Cr sources. One of organic-Cr sources known is fungi contained high Cr. This organic-Cr was incorporated to fungi protein. Information about the best fungi or microorganisms used in organic-Cr production is still limited. The objective of this experiment was to study organic-Cr production using different kinds of fungi as Cr carrier. Organic-Cr production was conducted in a 4x3 factorial completely randomized experimental design with 3 replications. Four fungi used as carriers in organic-Cr production as the first factor were Saccharomyces cerevisiae, Aspergillus oryzae, Rhizopus oryzae and "ragi tape". The second factor was levels of Cr addition (500, 1000, 1500 mg/kg). Addition of 1000 mg Cr/kg substrate gave the highest Cr incorporation to fungi protein (484 mg/kg) and Cr incorporation efficiency (21.1%). The best fungi used as carrier in organic-Cr production was Rhizopus oryzae which gave the highest Cr incorporation (488 mg/kg) and incorporation efficiency (24.7%). Key words: organic-Cr, production, Saccharomyces cerevisiae, Aspergillus oryzae, Rhyzopus oryzae, "ragi tape"

Inkorporasi Kromium oleh Fungi Ganoderma lucidum dengan Limbah Industri Kelapa Sawit Sebagai Substrat F Agustin; T Toharmat; D Evvyernie; D Taniwiryo; S. Tarigan; I Prihantono
Media Peternakan Vol. 33 No. 1 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The aim of this study was to determine the effective Cr (chromium) level for Ganoderma lucidum growth in solid state fermentation of oil palm by-product. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 4, 6, and 8 weeks). The treatments were allocated in a factorial 7x5 of complete randomized design with four replications. Inoculant of G. lucidum was grown in potato dextrosa agar (PDA) medium for 10 days and then innoculated to substrate which have been autoclaved and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 65%. Growth media of G. lucidum was diluted with aquades and the supernatant was analysed for its Cr content. The result showed that the addition of Cr up to 3000 ppm into the medium stimulated the G. lucidum growth in all experimental condition. The Cr ions were incorporated into the media and G. lucidum cells during fermentation. Incorporation of chromium by G. lucidum was higher in oil palm by-product substrate with 3000 ppm Cr than the others. It is concluded that Cr can be incorporated into the G. lucidum cells during fermentation. The effective level of Cr for G. lucidum growth was 3000 ppm with efficiency of Cr incorporation 68.23% in 8 weeks fermentation and chromium in protein of fermentation product was 12.01%. Key words: chromium, Ganoderma lucidum, oil palm by-product

Nutritive Value of Coffee Husk Fermented with Pleurotus ostreatus as Ruminant Feed I Badarina; D Evvyernie; T Toharmat; E N Herliyana; I K Darusman
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Coffee husks is an abundant crop residue but the content of anti nutritional substances such as caffeine, tannin, and lignin limit its utilization as feed ingredients. Higher fungi such as Pleurotus ostreatus have the ability to biotransform lignocellulosic materials through their extracellular enzyme activities. This study was carried out to assess the effect of solid state fermentation by using P. ostreatus on nutrient composition of coffee husk and to evaluate its potency as ruminant feed in vitro. The in vitro experiment was conducted to determine fermentability of treated coffee husk. The usage rate of fermented coffee husk was mimicked feeding level to mid lactation dairy cows; 0%, 10%, 20%, 30%, and 40% (R0 to R4). Fermentation of coffee husk by P. ostreatus increased its protein, from 10.36% to 12.14%, and cellulose, from 19.51% to 24.80%, and decreased its lignin, from 65.42% to 45.04%, tannin from 1.02% to 0.18%, and caffeine, from 1.39% to 0.20%, concentrations. There were no differences in ruminal pH and N-ammonia production but volatile fatty acid production and dry matter digestibility decreased as the fermented coffee husk level increased. The ruminal protozoa population in fermented coffee husk diets was lower than the control diets (P<0.05). In conclusion, it is possible to use 20% of fermented coffee husk in the ration.

The Incorporation of Chromium in Rice Straw Fermented with Ganoderma lucidum F Agustin; T Toharmat; D Evvyernie; D Taniwiryono; S Tarigan
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The research was designed to evaluate chromium incorporation by Ganoderma lucidum in rice straw substrate supplemented with chromium chloride. Treatments were combination of Cr level (0, 500, 1000, 1500, 2000, 25000, and 3000 ppm) and fermentation time (0, 2, 3, and 4 wk). The treatments were arranged in a factorial 7 x 4 and allocated in completely randomized design with three replications. G. lucidum was grown in potato dextrose agar (PDA) medium for 10 days and was inoculated to the substrate which have been sterilized and mixed with CrCl3.6H2O. The moisture of substrate was maintained at 70%. Fiber and protein components of growth media of G. lucidum was determined and analyzed for their Cr content. The finding result showed that addition of Cr up to 3000 ppm into the substrate stimulated the G. lucidum growth. Chromium was incorporated into the fiber and protein components of the growth substrate of G. lucidum during fermentation. Incorporation of Cr into the protein of substrate containing3000 ppm Cr was highest when fermented for 4 wk. Protein component of substrate contained 9.29% Cr while in NDF and ADF was 27.20% and 10.55% Cr, respectively. It is concluded that Cr was incorporated into the G. lucidum cells during fermentation.

Roles of Dietary Cobalt and Administration of Mixed Rumen Bacteria in Regulating Hematological Parameters of Pre-weaning Twin Lambs T Adelina; A Boediono; I G Permana; T R Wiradarya; D Evvyernie; T Toharmat
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Cobalt (Co) is required by rumen microorganism for vitamin B12 synthesis. Vitamin B12 is an important cofactor for methionine synthesis and gluconeogenesis. In young ruminants up to 6–8 wk old, the rumen has not been completely developed and rumen microorganisms are not ready to supply vitamin B12. The aim of this research was to determine the potency of mixed rumen bacteria and dietary supplementation of Co and its effect on plasma glucose, blood minerals (Co, Fe, and Zn) concentrations, and hematology of pre-weaning twin lambs. Twelve one month-old local twin lambs were assigned to 4 groups in a randomized complete block design. Lambs were fed cow milk at 10% body weight, adjusted weekly for 80 d. Mixed rumen bacteria were offered at 15 mL/d (8.295x1010 cfu). Dietary treatments were: 1) basal diet (Control), 2) basal diet + 1 mg/kg DM cyanocobalamin (VitB12) and 3) basal diet + 1 mg/kg DM of Co + administration of 15 mL mixed rumen bacteria (CoBac). There were no treatment effects on neither plasma glucose and blood mineral concentrations nor hematological profiles. This study demonstrated that pre-weaning twin lambs are not responsive to supplementation of Co and administration of mixed rumen bacteria.

In Vitro Rumen Fermentation and Anti Mastitis Bacterial Activity of Diet Containing Betel Leaf Meal (Piper betle L.) A A Yamin; A Sudarman; D Evvyernie
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aims of this experiment was to study the inhibition effect of betel leaf meal (BLM) addition into concentrate diet on mastitis causing bacteria and on rumen fermentation condition. The study consisted of five dietary treatments of BLM level in concentrate feed, i.e., 0%, 2%, 4%, 6%, and 8% and four replicates of each treatment. The treatment diets together with napier grass in ratio of 40 : 60 were fermented using rumen liquor. All treatments were examined their antibacterial activity before and after fermentation. After four hours fermentation, supernatant of each samples were analyzed for VFA, NH3, number of bacteria and protozoa. Dry matter (DM) and organic matter (OM) digestibility were analyzed after 48 h fermentation. The results showed that before fermentation, 8% BLM addition caused the bigest (P<0.05) inhibition diameter of Staphylococcus spp. growth compared to other lower levels. However after fermentation there were no significant differences among the addition levels of BLM. Two per cent of BLM addition produced higher VFA (P<0.05) than the other addition levels. Ammoniaconcentration, dry matter (DM) and organic matter (OM) digestibility were not different among the treatments. Addition of BLM significantly (P<0.01) decreased protozoa number, but did not affect bacterial count. It is concluded that the addition of 2% BLM in concentrate feed can be used effectively to inhibit the growth of mastitis causing bacteria (Staphylococcus spp.) and does not disturb rumen fermentation condition.

Digestibility, Milk Production, and Udder Health of Etawah Goats Fed with Fermented Coffee Husk Irma Badarina; D. Evvyernie; T. Toharmat; E.N. Herliyana; L.K. Darusman
Media Peternakan Vol. 38 No. 1 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was carried out to assess the utilization of coffee husk fermented by Pleurotus ostreatus as feed supplement by measuring the digestibility, milk production and udder health of Etawah goats suffered from subclinical mastitis (+1). There were three experimental diets consisted of T0 (control diet/basal diet without fermented coffee husk), T1 (basal diet with 6% fermented coffee husk) and T2 (basal diet with 6% fermented coffee husk soaked in crude palm oil for an hour before using). Basal diet consisted of napier grass (60%) and concentrate (40%). The results showed that supplementation of lactating Etawah does with fermented coffee husk did not affect the palatability of the diets, but increased the protein and crude fiber consumption (P<0.05). There was no significant effect on nutrient digestibility and milk production while milk composition (protein, fat, total solid) increased in supplemented groups (P<0.05). The persistency of milk production and the somatic cells count were not different. There was an improvement of somatic cells count on supplemented groups. In conclusion, fermented coffee husk could be used as feed supplement without any negative effects on digestibility and milk production. The positive effects to udder health could be expected from including fermented coffee husk in diets.Key words: coffee husk, Etawah goat, fermented, performance, supplement

Co-Authors . Alamsyari . SURYANI A Boediono A Haziq A K Agustina A K Agustina A Rosmalia A Sudarman A.P Sari Abdul Alim Yamin Adeliza, Muhammad Farhan Hilmy Amanda, Ridha Aini Apriliana, Tri Wahyu Asep Sudarman D E Yolanda D Khairunisa D M Fassah D Taniwiryo D Taniwiryono D. Diapari DADIK PANTAYA Despal Despal DEWI APRI ASTUTI Dian Anggraeni Dian Purwanti Dianingtyas, Brilian Desca Dwi Sisriyenni E N Herliyana E. N. Herliyana E.N. Herliyana Edi Kurniawan Elis Nina Herliyana Endah Yuniarti F Agustin G. E. Tresia H. Harmini I Badarina I G Permana I G Permana I K Darusman I Komang Gede Wiryawan I Prihantono I Prihantoro I. Badarina Idat Galih Permana Irma Badarina Irma Badarina Iwan Prihantoro Iwan Prihantoro Junita Purwanti K. Winaga L Abdullah L Abdullah L Abdullah L. K. Darusman L.K. Darusman Lilis Riyanti N Rahayu N.S Yunitasari P. D. M. H. Karti Putri, Dini Andani R, A R. Tiuria Ramadhani, Rizka Rika Zahera S Suryahadi S Tarigan S. M. Yantika S. Tarigan Shilvia, S Shofiah, Maya Suharti, Sri Suryahadi (Suryahadi) Suryahadi Suryahadi Suryahadi Suryahadi Suryani . Suryani . T adelina T R Wiradarya T Sutardi T Toharmat T Toharmat T Toharmat T. Toharmat Toto Toharmat W D Astuti Y. Widiawati Yuli Retnani Zikri Aulia Rahman

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