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All Journal Jurnal Kimia Terapan Indonesia ANNALES BOGORIENSES
Nurul Fitri Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Antimicrobial Activity of Selenium Nanoparticles Synthesized by Actinomycetes Isolated from Lombok Island Soil Samples Shanti Ratnakomala; Nurul Fitri Sari; Fahrurrozi Fahrurrozi; Puspita Lisdiyanti
Jurnal Kimia Terapan Indonesia Vol 20, No 1 (2018)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.255 KB) | DOI: 10.14203/jkti.v20i1.374

Abstract

AbstractA total of 98 actinomycetes were isolated from the soil and litter samples collected from the cacao and coffee plantation in Lombok Island, West Nusa Tenggara, Indonesia. These isolates were screened for their antimicrobial activity. Among 98 isolated strains, only 24 isolates showed antimicrobial activity against test microorganisms of which 20.4% were active against Bacillus subtilis BTCC B-612, 14.3% against Staphylococcus aureus BTCC B-611, and 5.1% against Escherichia coli BTCC B-609. Out of these 24 isolates, 3 were found to be able to grow in medium containing 3 mM Selenium oxide of which the culture were changed color to red. Two of the best strains, L-155 and L-156, were selected for assessing production of Selenium nanoparticles. Bioreduction of selenium nanoparticles was confirmed by UV–visible spectrophotometer which showed peak between 300 and 320 nm. Biosynthesized selenium nanoparticle from isolate actinomycetes L-155 and L-156 were found to have a broad spectrum of activity against the tested microorganisms: Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, and Candida albicans. This study showed rapid and eco-friendly synthesis of selenium nanoparticles from soil actinomycetes. Most of these active isolates revealed to possess antibacterial property.
In Vitro Ruminal Fermentation and Degradability of Rice Husk on Rice Bran Substitution Rusli Fidriyanto; Roni Ridwan; Wulansih Dwi Astuti; Rohmatussolihat Rohmatussolihat; Nurul Fitri Sari; Muh Watman; Yantyati Widyastuti
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.50-58

Abstract

Rice bran is a by-product of the rice milling process and has been well used as livestock feed. Rice bran is often adulterated with rice husk. The objective of this study was to evaluate the in vitro ruminal fermentation characteristics of rice bran with various compositions of rice husk and assess the relationship between rice husk addition and rice bran quality. The experiment was arranged in a completely randomized design with rice husk addition as a factor and three replications. Data of proximate value, gas production, ruminal degradability, and volatile fatty acid production were analyzed by analysis of variance. Moreover, significant effects of each treatment in the in vitro fermentation were further analyzed by Duncan's multiple range test (P<0.05). It was shown that the addition of rice husk to rice bran could increase acetic acid level, but it reduced potential gas production, gas production rate, organic matter and dry matter digestibility, and propionic acid level. Interestingly, the linear regression of dry matter digestibility, organic matter digestibility, and potential gas production showed the high adjusted R2 values. Moreover, this study also revealed that 10% of rice husk substitution on rice bran could significantly reduce the dry matter digestibility.
Co-Authors Fahrurrozi Fahrurrozi Muh Watman PUSPITA LISDIYANTI Rohmatussolihat Rohmatussolihat Roni Ridwan Rusli Fidriyanto Shanti Ratnakomala Wulansih Dwi Astuti Yantyati Widyastuti