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All Journal Jurnal Ilmu Ternak Veteriner AGRIC Annales Bogorienses
Roni Ridwan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Social Sciences Veterinary

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Utilization of Probiotic and Organic-Cn on Ruminal Ecosystem In Vitro Wulansih Dwi Astuti; Roni Ridwan; Baharuddin Tappa
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.992 KB) | DOI: 10.14334/jitv.v12i4.493

Abstract

Improvement of nutrient intake was needed to increase cattle productivity. Probiotics and organic Cr, as feed additives, might have beneficially improve ruminant microbial ecosystem, and optimize digestion process by rumen microbes. The objective of this study was to examine the effects of combination between probiotic or mixed probiotcs and organic Cr on rumen fermentation activity. An in vitro study was held based on randomized block design with 5 treatments and 3 replications. The treatments were (A) Control ration, (B) Control ration + probiotic TSD 10, (C) Control ration + probiotic TSD 10 + organic Cr, (D) Control ration + mixed probiotics, and (E) Control ration + mixed probiotics + organic Cr. Organic Cr concentration was supplemented 2 ppm, whereas probiotics supplement contained 109 cfu/g. Supplementation of probiotic and organic Cr increased dry matter and organic matter digestibility but decreased total VFA and NH3 concentration. Treatment E (combination between mixed probiotics and organic Cr) gave the highest dry matter (52.45 %) and organic matter (51.96 %) digestibilities. It also tended to increase NH3 and total VFA production. Supplementation of single or mixed probiotics showed no difference for dry matter and organic matter digestibilities, and proportion of individual VFA. Mixed probiotics gave higher VFA and NH3 concentration compared to single probiotic. Probiotics supplementation resulted in a tendency for higher acetate proportion, while organic Cr supplementation resulted in a tendency of higher propionate proportion. Key Words: Probiotic, Organic Cr, Ruminal Microbes, VFA
PENGARUH SUBSTITUSI RUMPUT GAJAH DENGAN LIMBAH SERAI PADA PAKAN RUMINANSIA TINGGI HIJAUAN TERHADAPKECERNAAN DAN PRODUKSI METAN SECARA IN VITRO Rusli Fidriyanto; Gunawan Priadi; Yashanti Berlinda Paradisa; Wulansih Dwi Astuti; Roni Ridwan; Rohmatussolihat Rohmatussolihat; Ki Ageng Sarwono; Muh Whatman; Yantyati Widyastuti
Agric Vol. 33 No. 2 (2021)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2021.v33.i2.p103-114

Abstract

Serai merupakan bahan baku untuk produksi minyak serai. Indonesia masuk dalam sepuluh negara terbesar produsen minyak serai di dunia. Industri pengolahan minyak serai akan menghasilkan limbah serai. Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi rumput gajah (Pennisetum purpureum) dengan limbah serai terhadap fermentasi rumen secara in vitro. Penelitian ini menggunakan rancangan acak kelompok dengan 5 perlakuan (100R: limbah serai 0%, 75R: limbah serai 25%, 50R: limbah serai 50%, 25R: limbah serai 75%, 0R: limbah serai 100%) dan 4 waktu pengambilan rumen yang berbeda sebagai kelompok. Hasil penelitian menunjukkan bahwa substitusi rumput dengan limbah serai tidak memberikan perbedaan yang signifikan terhadap kadar bahan kering, namun dapat menurunkan kadar protein dan meningkatkan serat kasar serta lemak kasar secara signifikan (P<0,05). Hasil analisis keceranaan in vitro terhadap aktivitas fermentasi rumen, menunjukkan bahwa substitusi serai tidak mempengaruhi pH, kecepatan produksi gas, asam butirat, asam valerat, asam isobutirat, dan asam isovalerat baik dari segi jumlah maupun proporsi. Substitusi rumput dengan limbah serai sebesar 25% (75R) tidak menghasilkan penurunan kadar bahan organik, produksi gas potensial dan produksi metan (jam ke-24) namun terjadi penurunan kadar protein dan meningkatkan lag time secara signifikan (P<0,05). Penggunaan serai sebesar 50% dapat menurunkan produksi gas metan secara signifikan (P<0,05). Peningkatan konsentrasi limbah serai yang digunakan menyebabkan terjadinya perubahan profil VFA parsial yang disebabkan oleh adanya perubahan komposisi asam asetat. Penurunan rasio asam asetat/ propionat (A/P) secara signifikan terjadi pada penggunaan serai sebesar 100%. Penggunaan limbah serai dapat mensubtitusi rumput gajah hingga 25% pada pakan tinggi hijauan tanpa terjadi penurunan pada produksi gas potensial, kecernaan dan total VFA.
The Screening of Antibacterial Activity of Selenium Nanoparticles Produced by Indonesian Marine Actinomycetes Shanti Ratnakomala; Akhirta Atikana; Nurul Fitri Sari; Roni Ridwan; Fahrurrozi Fahrurrozi; Puspita Lisdiyanti
Annales Bogorienses Vol. 29 No. 2 (2025): Annales Bogorienses
Publisher : National Research and Innovation Agency (BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/ann.bogor.2025.2921

Abstract

Actinomycetes strains can reduce SeO3-2 (selenite) and (or) SeO4-2 (selenate) to Se0, which is less toxic by forming selenium nanoparticles (SeNPs). This study aimed to screen the actinomycetes strains capable of biosynthesizing and producing SeNPs with antibacterial activity. This study showed that the antibacterial properties of SeNPs were synthesized biologically by the three strains of actinomycetes, e.g., the strains BLH 5-21, BLH 5-22, and BLH 12-1. The nanoparticles synthesized by the three actinomycetes strains were active against the pathogenic tested bacteria: Bacillus subtilis BTCC B-612, Staphylococcus aureus BTCC B-611, Micrococcus luteus BTCC B-552, Escherichia coli BTCC B-609, and Candida albicans Y-033. The PSA and SEM images showed that the selenium nanoparticles from strain BLH 12-1 had a spherical shape, and their mean diameter was between 64-110 nm, with an average size of 98 nm. The biogenic selenium nanoparticles have exhibited promising application prospects in medicine, biosensors, and environmental remediation. These microorganisms might be explored as the potential of microbial nano factory for synthesizing metal nanoparticles.
Co-Authors Akhirta Atikana Baharuddin Tappa Fahrurrozi Fahrurrozi Gunawan Priadi Ki Ageng Sarwono Muh Watman Muh Whatman Nurul Fitri Sari PUSPITA LISDIYANTI Rohmatussolihat Rohmatussolihat Rohmatussolihat Rohmatussolihat Rusli Fidriyanto Rusli Fidriyanto Shanti Ratnakomala Wulansih Dwi Astuti Wulansih Dwi Astuti Yantyati Widyastuti Yantyati Widyastuti Yashanti Berlinda Paradisa