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All Journal BALI MEDICAL JOURNAL (BMJ) INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES
N. T. Suryadhi
School of Public Health, Faculty of Medicine Udayana University, Bali-Indonesia
Medicine & Pharmacology

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EPIDEMIOLOGICAL AND MOLECULAR ANALYSIS OF TOXOPLASMA GONDII IN FAECAL SAMPLES OF CATS OBTAINED FROM HOUSE OF MATERNAL IN BALI Subrata, I M.; Suryadhi, N. T.; Astawa, N. Mantik; Damriyasa, I M.
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

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Abstract

Background: toxoplasmosis is a zoonotic-parasite disease caused by T.gondii that spread in many countries. Recently, this disease is one of the major public health problems associated with severe enocomical and social impacts such as miscarriages, hydrocephalus, blindness and mental retardations. The human parastic infection are closely related with the presence of infected cats around their houses, in which the animals are the definitive host releasing the parasites into the environment via their feces. The aim of this research was to determine the correlation between morphological prevalence and molecular detection of T.gondii in cats feces. Methods: detection of molecular was carried out by applying microscopic and a specific polymerase chain reaction (PCR). A case-control study using 40 maternal with positive serology of T. gondii and 40 maternal with negative serology were selected by purposive sampling method. Similarly, 80 faecal sample of cats were also obtained from each house of the women. The major risk factors considered as the important rule for human toxoplasmosis such as age, education, occupation, contact with soil, cat’s age, un-owned cats and oocyst-positive feces were determined by questionnaire. Results: The epidemiological analysis showed that the highest risk factor was oocyst-positive feces (OR= 8.143; p= 0.003), followed by education (OR= 3.414; p=0.045), contact with soil (OR= 2.255; p= 0.073), un -owned cats (OR= 2.25; p= 0.210), age (OR=2.23; p=0.074), occupation (OR= 1.556; p= 0.348) and cat’s age (OR= 1.138; p= 0.799). However, the pregnant women with serologically positive to T. gondii had a very high probability of miscarriages (OR= 18.857; p= 0.0001) compared to whom with serologically negative. Moreover, logistic regression model analysis revealed that only oocyst-positive feces and education contributed a significant factor in causing toxoplasmosis among pregnant women. Microscopic observation of 80 faecal samples indicated that only 14 (17.5%) contained typical oocyst of T. gondii. All of the 8 oocyst of T. gondii positive-faecal samples were further confirmed with PCR, and we found that 4 (50%) were positive. Conclusion: This study concluded that the presence of oocyst-positive feces in the environment in conjuction with education level were considered to play significant contribution to the accident of toxoplamosis in pregnant women.
COMBINATION OF TEMPEH AND CARROT PREVENT ATHEROSCLEROSIS WISTAR RAT: Indicated by Increase of HDL and Total Antioxidant, Decrease LDL, F2-Isoprostan, and IL-6 Ari-Agung, I G. A.; Suryadhi, N. T.; Mantik, N. A.; Suter, I K.; Partama, and I. B. G.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013
Publisher : Udayana University

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Coronary heart disease (CHD) is the leading cause of death, either in developed and developing countries. The disease is stimulated by the present of atherosclerosis. This study aims to investigate supplementation of combined tempeh M-2 and carrot to prevent atherosclerosis wistar rat by increases HDL and TAC, decreases LDL, F2-Isoprostan, and IL-6.  This was a true experimental study with the factorial completely randomized post-test only control group design.  variables such as KN (standard feeding / pellets (50 g / kg bw / day), KP: pig lubrication: pellets (1: 9) (50 g / kg bw / day), T : lubrication pig: pellets (1: 9) with tempeh M -2 (20 g / kg bw / day), W: pig lubrication: pellets (1: 9) with carrots (20 g / kg bw / day), and TW: pig lubrication: pellets (1: 9) with tempeh M-2 (20 g / kg bw / day), and carrots (20 g / kg bw / day).  Dependent variables in this study are serum HDL,serum TAC, LDL serum, urine F2-Isoprostan, and plasma IL-6 (with Elisa Method). Data were analyzed using the F test (two-way ANOVA), followed by LSD test. Descriptive research was also conducted in this study in order to find out the change of aortic histopathologic. The highest average levels of HDL, TAC contained on TW, which respectively amounted 68.640 ± 0.50 mg / dl, 1.454 ± 0.01 nM / mL. It showed highly significant differences (p<0.01) in the various treatments.  TW treatment showed highly significant interaction effect (p<0.01) were observed for all parameters except for HDL. Average levels of LDL, F2- Isoprostan, and IL-6  lowest in the treatment TW, which respectively amounted  20.718 ± 1.33 mg / dl, 0.720 ± 0.065 ng / dl, 35.328 ± 1.000 pg/dl, showed highly significant differences (p<0.01) in the various treatments. It can be concluded that supplementation with a combination of tempeh M-2 with carrots give the best effect, can increases HDL and TAC, and can decreases LDL, F2-Isoprostan, IL-6 significantly, and may change the hystopathology  structure of aorta from endotel dysfunction to become normal.
PENGARUH PENYIANGAN DAN SUHU PENYIMPANAN TERHADAP MUTU KIMIAWI, MIKROBIOLOGIS DAN ORGANOLEPTIK IKAN TONGKOL (Auxis tharzard, Lac) Suranaya Pandit, I G.; Suryadhi, N. T.; Arka, I. B.; Adiputra, N.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 1, No. 3 Desember 2007
Publisher : Udayana University

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Abstract

Ikan tongkol merupakan salah satu bahan pangan yang dikonsumsi masyarakat dan jika dibiarkanpada suhu kamar, maka terjadi proses penurunan mutu menjadi busuk. Ikan yang sudah mengalami prosespembusukan, bila dikonsumsi dapat menimbulkan keracunan (Histamine fish poisoning). Keracunan inidisebabkan oleh kontaminasi bakteri pathogen dengan dekarboksilasi asam amino histidin oleh enzimhistidin dekarboksilase menghasilkan histamin. Bakteri ini banyak terdapat pada anggota tubuh manusiayang tidak higienis, kotoran/tinja, isi perut ikan, insang serta peralatan yang tidak bersih.Penelitian eksperimental dengan pola faktorial, yaitu faktor P adalah faktor penyiangan dengan 2 taraf,tanpa penyiangan dan penyiangan, sedangkan faktor T adalah suhu penyimpanan dengan 3 taraf yaitu suhupenyimpanan 30oC, 15oC dan 0oC.Analisis statistik terhadap mutu kimiawi seperti kadar histamin, kadar total volatil bases (TVB) dantrimetilamin (TMA) menunjukkan perbedaan nyata (P<0,05) pada pengaruh penyiangan dan suhupenyimpanan. Terjadi peningkatan kadar histamin, kadar TVB dan TMA selama penelitian. Selamapenelitian terjadi peningkatan jumlah koloni bakteri, jumlah Coliform, kecuali bakteri Vibrioparahaemolyticus negatif. Perlakuan penyiangan dan suhu penyimpanan 0oC memiliki mutu kimiawi,mikrobiologis terbaik sampai hari ke 10 serta masih diterima panelis.Hubungan antara kadar histamin dengan jumlah bakteri mempunyai hubungan sangat kuat, ditunjukkandengan nilai r ? 0,7 kecuali kadar histamin dengan waktu memiliki hubungan agak lemah r ? 0,5.Keamanan ikan tongkol dengan penerapan teknologi tepat guna berupa tanpa penyiangan danpenyiangan pada suhu 30oC hanya aman untuk dikonsumsi sampai hari ke 0. Perlakuan tanpa penyiangandan suhu penyimpanan 15oC aman sampai hari ke 4, sedangkan dengan penyiangan aman sampai hari ke 6.Untuk perlakuan tanpa penyiangan dan penyiangan dengan suhu penyimpanan 0oC aman sampai hari ke 10.
Co-Authors I G. A. Ari-Agung I Gde Suranaya Pandit I K. Suter I Made Damriyasa I Made Subrata I NYOMAN MANTIK ASTAWA I. B. Arka Ida Bagus Gaga Partama N. A. Mantik N. Adiputra