Article Per Year (5 Year)
p-Index From 2020 - 2025
0.61
P-Index
This Author published in this journals
All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Medik Veteriner Current Trends in Aquatic Science International Journal of Interreligious and Intercultural Studies Jurnal Kedokteran Hewan
I Made Damriyasa
Faculty Of Veterinary Medicine, Udayana University
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

Published : 58 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 58 Documents
Search

 1   2   3   4   5 
EPIDEMIOLOGICAL AND MOLECULAR ANALYSIS OF TOXOPLASMA GONDII IN FAECAL SAMPLES OF CATS OBTAINED FROM HOUSE OF MATERNAL IN BALI Subrata, I M.; Suryadhi, N. T.; Astawa, N. Mantik; Damriyasa, I M.
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.401 KB)

Abstract

Background: toxoplasmosis is a zoonotic-parasite disease caused by T.gondii that spread in many countries. Recently, this disease is one of the major public health problems associated with severe enocomical and social impacts such as miscarriages, hydrocephalus, blindness and mental retardations. The human parastic infection are closely related with the presence of infected cats around their houses, in which the animals are the definitive host releasing the parasites into the environment via their feces. The aim of this research was to determine the correlation between morphological prevalence and molecular detection of T.gondii in cats feces. Methods: detection of molecular was carried out by applying microscopic and a specific polymerase chain reaction (PCR). A case-control study using 40 maternal with positive serology of T. gondii and 40 maternal with negative serology were selected by purposive sampling method. Similarly, 80 faecal sample of cats were also obtained from each house of the women. The major risk factors considered as the important rule for human toxoplasmosis such as age, education, occupation, contact with soil, cat’s age, un-owned cats and oocyst-positive feces were determined by questionnaire. Results: The epidemiological analysis showed that the highest risk factor was oocyst-positive feces (OR= 8.143; p= 0.003), followed by education (OR= 3.414; p=0.045), contact with soil (OR= 2.255; p= 0.073), un -owned cats (OR= 2.25; p= 0.210), age (OR=2.23; p=0.074), occupation (OR= 1.556; p= 0.348) and cat’s age (OR= 1.138; p= 0.799). However, the pregnant women with serologically positive to T. gondii had a very high probability of miscarriages (OR= 18.857; p= 0.0001) compared to whom with serologically negative. Moreover, logistic regression model analysis revealed that only oocyst-positive feces and education contributed a significant factor in causing toxoplasmosis among pregnant women. Microscopic observation of 80 faecal samples indicated that only 14 (17.5%) contained typical oocyst of T. gondii. All of the 8 oocyst of T. gondii positive-faecal samples were further confirmed with PCR, and we found that 4 (50%) were positive. Conclusion: This study concluded that the presence of oocyst-positive feces in the environment in conjuction with education level were considered to play significant contribution to the accident of toxoplamosis in pregnant women.
Lactose-Astaxanthin Increases Green Jungle Fowl’s Sperm Motility and Reduces Sperm DNA Fragmentation During 5o Celsius Storage Bebas, Wayan; Pemayun, Tjok Gede Oka; Damriyasa, I Made; Astawa, I Nyoman Mantik
BALI MEDICAL JOURNAL Vol 4 No 3 (2015)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.155 KB)

Abstract

Background: Good quality of semen is required for artificial insemination technology in ex-situ conservation efforts of green jungle fowl. This study was aimed to investigate semen quality of green jungle fowl during storage at 5oC for 48 hours with the addition of combination lactose astaxanthin in egg yolk phosphate dilution. Method: The semen used in the study was collected from eight healthy male green jungle fowls by using massage techniques. The semen quality was analyzed with macroscopic and microscopic examinations. The semen was diluted with egg yolk phosphate with the addition of 0.6% lactose, 0,004% astaxanthin and combination 0.6% Laktose-0,004% astaxanthin, and was stored at 5oC for 48 hours. Following 48-hour treatment, the semen quality was evaluated based on its progressive motility, and DNA fragmentation. Data were firstly analyzed by using analysis of variance (ANOVA), and were then proceeded by using Duncan Multiple Range test. Results: The results showed that the progressive motilities of semen diluted in 0.6% lactose combined with astaxanthin 0.004% %, (79,66 + 1.50%) was significantly higher than those diluted in 0.6% lactose (66,77 + 2.16%,) and in astaxanthin 0.004% (68,11 + 3.01 %). The DNA fragmentation of semen diluted inn 0.6% lactose combined with astaxanthin 0.004% %, (7,55 + 1,66%) was significantly lower than those diluted in 0.6% lactose (12,33 + 1,93%) and in astaxanthin 0.004% (13,55 + 1,81%). Conclusions: In conclusion, the combination of l 0.6% lactose -astaxanthin 0.004% showed the best results for progressive motility, and DNA fragmentation.
Administration of Leptin Increases the Level of Follicle Stimulating Hormone (FSH) and Development of Ovarian Follicles in Postpartum Anestrus of Bali Cattle Pradnyana, Dewi Indira Laksmi; Pemayun, Tjok G. O.; Damriyasa, I Made; Dharmawan, N. S.
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.922 KB)

Abstract

Background: Leptin is a metabolic signal that plays a role in reproduction through the Hypothalamic-PituitaryOvarian axis. The aim of this study is to determine the effect of leptin on the levels of FSH and development of ovarian follicles of post-partum anestrus in Bali cattle. Method: Postpartum anestrus in Bali cattle were divided into three groups of nine; group 1 (control) was injected with physiological saline, group 2 (treatment I) and group 3 (treatment II) were injected with recombinant o-leptin 100 µg and 200 µg/head respectively. Injection was done twice with an interval of 12 hours. Blood sample was collected via the jugular vein at intervals of 12 hours before the start of treatment until signs of estrus had appeared. Follicle diameter was measured using ultrasonography before treatment and during estrus. Results: The average levels of FSH before treatment for group 1, group 2, and group 3 were 11.3 + 0.36 mIU/ml, 11.6 + 0.40 mIU/ml, and 11.4 + 0.28 mIU/ml respectively. While at the end of the research, the levels of FSH were 11:02 + 0.39 mIU/ml, 13.0 + 0.48 mIU/ml, and 13.47 + 1.05 mIU/ml respectively. Ovarian follicle diameter for group 1, group 2, and group 3 before treatment were 5.10 + 0.41 mm, 5.21 + 0.36 mm, and 5.00 + 0.37 mm respectively. Whereas at the end of the research, the ovarian follicle diameters were 4.92 + 0.40 mm, 8.37 + 0.43 mm, and 8.45 + 0.38 mm respectively. Conclusion: administration of o-leptin in postpartum anestrus of Bali cattle increases the level of FSH and ovarian follicle development.
CANTHARANTHUS ROSEUS LEAF EXTRACT ACCELERATES HEALING OF WOUND WISTAR RAT Anom-Dada, I K.; Berata, I K.; Putra-Manuaba, I. B.; Damriyasa, and I M.
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol 7 No 1 (2013): IJBS Vol.7 No.1 January-June 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.827 KB)

Abstract

Cantharantus roseus (C. roseus) is a medical plant that has been used to treat various diseases. This study was carried to determine C. roseus leaf extract able to acclerate wound healing in Wistar rat. Full skin-tickness round wounds were created on the back of the rats. Wound healing was evaluated after treated with 15% of a mixture of leaf extract of C. roseus and vaseline. The rats were divided into two groups, each group consist of 16 rats. Wound healing was assesed in five and fifteen days after treatment by measuring wound area, wound closure, and counting the new capillary vessel number (angiogenesis). The result of the study indicated that extract of C. roseus leaf accelerates the wound healing in Wistar rat. Coverage rate of wound is faster on treated group than control group (p<0.05). Wound closure and angiogenesis in early wound healing were higher in treated group than control group (p<0.05). These results suggested that the first phase of wound healing was shortened, and the proliferative and maturation phases were advanced by methanol extract of C. roseus leaf. Therefore, it can be concluded that C. roseus could be potential to help in topical management of wound healing.  
SENSITIVITY AND SPECIFICITY OF ELISA TEST USING 30 KDA RECOMBINANT ANTIGEN TO DETECT Toxoplasma gondii INFECTION IN PIG WITH MICE BIOASSAY AS A GOLD STANDARD Nyoman Adi Suratma1????, I Made Bakta2, I Made Damriyasa3 Adi Suratma, Nyoman; Bakta, I Made; Damriyasa, I Made
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 3, No. 1 Januari 2009
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.06 KB)

Abstract

Toxoplasmosis is a zoonotic disease caused by the protozoa Toxoplasma gondii.Primary infection in pregnant women can cause abortion, neonatal death or abnormalityof fetus. Accurate diagnosis is needed to prevent infection especially related to thepresence of cyst in the tissuesThe aim of this research was to study sensitivity and specificity of ELISAmethod with 30 kDa protein antigen to detect T.gondii infection in pig with micebioassay as gold standard.. Samples were 171 pigs slaughtered at pig slaughter house inDarmasaba BadungThe result showed that sensitivity of ELISA method was 100% and 90,7% inspecificity.Research about sensitivity using ELISA test to predict cysts presence in tissuewere needed in the future.
Distribusi Cacing pada Berbagai Organ Ikan Tongkol SILABAN, BERNA NATALIA; DAMRIYASA, I MADE; ADI SURATMA, NYOMAN
Indonesia Medicus Veterinus Vol.1 (1) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi dan intensitas infeksi cacing serta mengetahui distribusi cacing pada berbagai organ ikan tongkol (Auxis rochei) yang dipasarkan di Kedonganan, Badung. Telah dilakukan penelitian terhadap 35 ekor ikan tongkol (Auxis rochei) dengan pengamatan terhadap cacing yang ditemukan dengan menggunakan mikroskop, dan diidentifikasi berdasarkan morfologi umumnya (Nurhayati et al., (2007)), Rohde (2005), Yanong (2008)). Penelitian ini dilakukan di Laboratorium bersama Centre for Studies on Animal Diseases (CSAD) Bukit Jimbaran. Data yang berhasil dikumpulkan dianalisa secara deskriptif dan untuk mengetahui hubungan antara intensitas dengan panjang dan berat ikan digunakan uji Rank Spearman.Hasil penelitian didapatkan prevalensi infeksi cacing pada ikan tongkol (Auxis rochei) yang dipasarkan di Kedonganan, Badung adalah 100% dengan intensitas berkisar antara 4-339 ekor cacing dan rata-rata 31,4 serta distribusi infeksi cacing pada ikan tongkol (Auxis rochei) yang dipasarkan di Kedonganan, Badung terjadi pada berbagai organ diantaranya operculum, insang, rongga insang, lambung, usus, hati, sekum, gonad dan rongga tubuh ikan.
Prevalensi dan Distribusi Cacing Pada Berbagai Organ Ikan Selar Bentong FRISKA TAMBA, MORI; DAMRIYASA, I MADE; ADI SURATMA, NYOMAN; THEISEN, STEFAN
Indonesia Medicus Veterinus Vol 1 (4) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.704 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi dan distribusi cacingpada berbagai organ ikan selar bentong (Selar crumenophthalmus) yangdipasarkan di Kedonganan, Badung. Sampel yang digunakan adalah 35 ekor ikan selar bentong (Selar crumenophthalmus) yang berasal dari pasar ikan Kedonganan. Ikan diperiksa di laboratorium secara kasat mata yang dilanjutkan dengan pemeriksaan dibawah mikroskop. Selanjutnya data mengenai distribusi cacing pada berbagai organ yang didapat dalam penelitian dapat digunakan rancangan penelitian Crosss Sectional Study yang dilaporkan secara deskriptif. Hasil pengamatan terhadap sampel adalah ditemukannya 4 jenis parasit cacing, yaitu dari filum cacing Nemathelminthes (Anisakis spp, Camallanus spp, dan Acanthocephala) dan Plathyhelminthes (Digenea). Cacing Anisakiss spp ditemukan pada organ rongga perut, usus, pylorik, perut, dan gonad. Hal ini diakibatkan karena Anisakis spp pada ikan selar bentong (Selar crumenophthalmus) masih berupa larva yang hidupnya motil sehingga bisa berpindah tempat. Sedangkan Anisakis spp dewasa terdapat pada mamalia laut (lumba-lumba dan paus), dimana cacingnya sudah bersifat dormant/ menetap pada jaringan otot. Organ alami Camallanus spp adalah pada organ usus, tetapi dalam penelitian ini cacing Camallanus spp ditemukan pada organ gonad. hal ini disebabkan karena adanya migrasi cacing. Acanthocephala merupakan cacing berkepala duri, karena kekhasan tubuhnya yang memiliki proboscis yang dilengkapi dengan kait. Dalam penelitianini Acanthocephala ditemukan pada organ usus. Serta cacing lainnya dari filum Plathyhelminthes yaitu Digenea. Dimana Digenea memiliki ciri yang khas yaitu mempunyai oral sucker dan ventralsucker. Anisakis spp merupakan cacing yang paling banyak ditemukan yaitu 83,8% dari total jumlah cacing yang ditemukan. Sedangkan cacing Camallanus spp dan Acanthocephala merupakan parasit cacing yang paling sedikit ditemukan yaitu sekitar 0,95%. Serta cacing lainnya adalah Digenea yaitu sekitar 14,3%.
Penggunaan “Crude Antigen” Cysticercus Cellulosae untuk Menentukan Seroprevalensi Sistiserkosis pada Babi Stelen, I Dewa Ketut Raeyadi Purnama; Damriyasa, I Made; Swacita, Ida Bagus Ngurah
Indonesia Medicus Veterinus Vol 6 (4) 2017
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.578 KB)

Abstract

Sistiserkosis merupakan penyakit parasit zoonotik yang penting pada hewan dan manusia. Babi merupakan inang antara bagi perkembangan cacing ini dan dapat bertindak sebagai penular penyakit kepada manusia. Penelitian ini bertujuan untuk menentukan kadar protein antigen Cysticercus cellulosae dan penggunaan Crude antigen Cysticercus cellulosae untuk menentukan seroprevalensi sistiserkosis pada babi dengan metode Enzyme Linked Immunosorbent Assay (ELISA). Crude antigen dibuat dari larva cacing pita babi (Cysticercus cellulosae) dengan larutan Phosphate Buffer Solution (PBS), kadar protein crude antigen Cysticercus cellulosae diukur dengan Q-fluorometer. Optimalisasi uji ELISA dilakukan dengan cara checkerboard. Sebanyak 93 sampel serum babi asal Lembah Baliem, Papua diuji seroprevalensi sistiserkosis pada babi dengan uji ELISA. Hasil penelitian menunjukan bahwa kadar protein crude antigen Cysticercus cellulosae sebesar 876 µg/ml, pengenceran crude antigen Cysticercus cellulosae tertinggi 100 µg/ml, pengenceran tertinggi serum babi 1 : 50, dan pengenceran tertinggi konjugat 1 : 2000. Seroprevalensi sistiserkosis pada Lembah Baliem, Papua sebesar 10,75 %. Disarankan untuk melakukan monitoring dan surveilens sistiserkosis pada babi secara berkala untuk mencegah dan mengendalikan penyakit ini.
AKURASI METODE RITCHIE DALAM MENDETEKSI INFEKSI CACING SALURAN PENCERNAAN PADA BABI Dwi Suryastini, Kadek Ayu; Damriyasa, I Made; Dwinata, I Made
Indonesia Medicus Veterinus Vol 1 (5) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.877 KB)

Abstract

Penyakit cacing merupakan salah satu jenis penyakit parasit yang dapat menginfeksi. Cara mendiagnosa infeksi cacing selain dengan melalui gejala klinis dan pemeriksaan post mortem dapat juga dilakukan dengan pemeriksaan feses. Salah satu metode untuk pemeriksaan feses adalah dengan metode Ritchie. Tujuan dilakukannya penelitian ini adalah untuk mengetahui akurasi metode Ritchie pada pemeriksaan feses babi untuk mendeteksi infeksi cacing pada saluran pencernaan. Bahan-bahan yang dipakai dalam penelitian ini adalah sampel feses dan isi saluran gastrointestinal babi yang diambil dari Pegunungan Arfak dan Lembah Baliem dengan jumlah sebanyak 22 sampel. Pemeriksaan cacing dilakukan dengan pemeriksaan post mortem sedangkan pemeriksaan feses dilakukan dengan metode Ritchie kemudian dihitung nilai sensitifitas dan spesifisitas. Berdasarkan hasil yang diperoleh pada pemeriksaan post mortem dan pemeriksaan feses maka didapat nilai spesifisitas dan sensitifitas untuk masing-masing jenis cacing pada pemeriksaan feses dengan metode Ritchie secara keseluruhan nilai sensitifitas antara 0%-72% dan spesifisitas 9,09%-72%. Hasil pemeriksaan dengan metode Ritchie pada penelitian ini mempunyai tingkat sensitifitas yang tidak tinggi. Dari hasil yang didapatkan maka dapat disimpulkan untuk jenis cacing telur type Strongyl didapat sensitifitas 72,72% dan spesifisitas 9,09%, untuk cacing Macracanthorhyncus didapat sensitifitas 0% dan spesifisitas 75%, untuk cacing Strongyloides didapat sensitifitas 44,44 % dan spesifisitas 100%, Ascaris didapat sensitifitas 40% dan spesifisitas 64,71% untuk cacing Trichuris didapat sensitifitas 63,62% dan spesifisitas 63,63%.
Seroprevalensi Infeksi Toxoplasma gondii pada Babi di Lembah Baliem dan Pegunungan Arfak Papua YOGA LOKANTARA, I PUTU; DAMRIYASA, I MADE; DWINATA, I MADE
Indonesia Medicus Veterinus Vol 1 (4) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.846 KB)

Abstract

This study aims to determine the presence of Toxoplasma gondii in pigs at the Baliem Valley and at the Mountains of Arfak Papua. Using the Indirect test Haemaglutination Asay, Primary Cellognost * Toxoplasmosis ? H (IHA reagent Toxoplasmosis), Reagent Buffer, Buffer Serum, Toxoplasmosis Control Serum, positive (IgG), Toxoplasmosis Control Serum, negative, and Pig Serum. The results showed the presence of Toxoplasma gondii in pigs at the Baliem Valley is (75.9%) and at the Mountains of Papua Arfak amounts (25%),
Co-Authors Adryani Ris Akira Ito Anak Agung Gde Oka Dharmayudha Anak Agung Komang Suardana Anak Agung Sagung Kendran BERNA NATALIA SILABAN Desak Nyoman Dewi Indira Laksmi Dewa Putu Sahadewa Dharmawan N.S Dina Harumna Endang Wulandari Suryaningtyas Farika E. Y Gede Yudi Suryawan Handayani, Ni Made HERTATI ANRIANI LUBIS I Gede Mahardika I K. Berata I K. K. Agustina I Kadek Saka Wiryana I Kadek Swastika I Ketut Anom Dada I Ketut Berata I Ketut Suada I Made Angga Prayoga I Made Bakta I Made Dwinata I MADE INDRA PERMADI I Made Kardena I Made Merdana I Made Subrata I Nengah Kapti I NYOMAN ADI SURATMA I Nyoman Arsana, I Nyoman I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Sunita I Nyoman Wijaya Guna I PUTU YOGA LOKANTARA I Wayan Bebas I Wayan Masa Tenaya I Wayan Masa Tenaya, I Wayan Masa I Wayan Wahyudi, I Wayan I. B. Putra-Manuaba Ida Ayu Laksmi Puspita Dewi Ida Ayu Pasti Apsari Ida Bagus Gde Yudha Triguna Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya K. K. Agustina Kadek Agus Agra Arnawa Kadek Ayu Dwi Suryastini Kadek Dianiyanti Kadek Karang Agustina Ketut Budiasa Luh Dewi Anggreni MARIANA KRESTY FERDINANDEZ MORI FRISKA TAMBA Muhammad Ulqiya Syukron Munehiro Okamoto N. T. Suryadhi N.S. Dharmawan Ngakan Putu Anom Harjana Ni Ketut Ayu Juliasih Ni Luh Ayu Trisnayanti Ni Luh Gede Merry Cintya Laksmi Ni Luh Gede Sudaryati Ni Made Yunik Novita Dewi Dewi Nyoman Tigeh Suryadi PANDE KETUT YULIARI PRATIWI DEVI GM Putu Nara Kusuma Prasanjaya Putu Suma Githa Sanjaya Putu Sutisna sang gede purnama Sri Hartini STEFAN THEISEN Stelen, I Dewa Ketut Raeyadi Purnama Sudiartawan, I Putu Sumartono - Sumartono . Sumartono s Sumartono s Sumarya, I Made Tjok G. O. Pemayun, Tjok G. O. Tjok Gede Oka Pemayun, Tjok Gede Oka TRI KOMALA SARI Wayan Tunas Artama Wayan Tunas Artama Widayantari, Anak Agung Ayu Sauca Sunia