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All Journal Chimica et Natura Acta Agrin : Jurnal Penelitian Pertanian Jurnal Agroplasma Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis PERTANIAN TROPIK
Untung Susanto
Indonesian Center for Rice Research, Street 9th, Sukamandi, Subang 41256, West Java, Indonesia Phone (+62-260) 520157
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Materials Science & Nanotechnology

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IDENTIFIKASI POLIMORFIS MARKA-MARKA MOLEKULER YANG DIDUGA BERKAITAN DENGAN KARAKTER DAYA HASIL TINGGI PADA 30 GENOTIP PADI Carsono, Nono; Lukman, Pradita N.; Damayanti, Farida; Susanto, Untung; Sari, Santika
Chimica et Natura Acta Vol 2, No 1 (2014)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.438 KB) | DOI: 10.24198/cna.v2.n1.9141

Abstract

Identifikasi marka polimorfis yang diduga berasosiasi dengan karakter daya hasil tinggi sangat penting dilakukan guna aplikasi seleksi berbasis marka dalam rangka perakitan padi berdaya hasil tinggi. Penelitian ini dilakukan untuk mengidentifikasi 30 genotip padi yang diduga berdaya hasil tinggi dengan menggunakan marka SSR. Amplifikasi produk PCR dipisahkan dengan menggunakan gel agarose 3% atau polyacrylamide gel electrophoresis (PAGE) 8%. Tingkat keinformatifan marka dapat ditentukan dengan cara penghitungan Polymorphic Information Content (PIC). Hasil analisis menunjukan bahwa marka OSBLE3, RM 282, dan RM 259 memiliki nilai PIC ≥ 0,5. Marka SSR dan marka gen spesifik yang digunakan untuk mengidentifikasi padi berdaya hasil tinggi menunjukkan bahwa tiga genotip padi terseleksi dapat direkomendasikan sebagai tetua donor dalam persilangan diantaranya yaitu genotip #1 (Fatmawati), #3 (Inpari13), dan #30 (IPB 160-F-3-3-1). Marka molekuler yang digunakan dapat memperkirakan tingkat polimorfisme dan juga berguna untuk mengkonfirmasi genotip padi yang berpotensi daya hasil tinggi.
Respon Berbagai Varietas Padi pada Lahan Organik dengan System of Rice Intensification (SRI) di Sragen Barokah, Umi; Susanto, Untung
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 4, No 2 (2020): SEPTEMBER
Publisher : Kadiri University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v4i2.1065

Abstract

Rice is the most essential staple food of Indonesian. The strategy to meet these food needs is through efforts to increase productivity by means of agricultural intensification. One of efforts to increase productivity is the application of integrated technology using the System of Rice Intensification (SRI) method and organic cultivation. The research was conducted from March to June 2017 in Sambirejo, Sukorejo, Sragen. Forty rice varieties originating from the Center for Rice Research, Sukamandi, Subang, West Java were tested in this study. Planting was carried out by transplanting using 1 seedlings of 15 days after dispersing (HSS) / planting hole on a plot measuring 2 m x 2 m with a spacing of 20 cm x 20 cm. The research was organized using a completely randomized block design with four replications. Cultivation techniques are carried out organically with guidelines for integrated plant management. Observations were made on the character of the agronomic performance and yield. The results of the analysis of variance showed that there were differences between the varieties tested on all agronomic characters and yields. Varieties that responded well to organic land with the SRI system at the study site were Membramo, Sintanur, Inpari 13, Inpari 32 and Inpari 5 Merawu varieties, while the varieties that responded poorly to the study were Batutegi and Lusi varieties.Padi merupakan makanan pokok masyarakat Indonesia yang mutlak harus terpenuhi. Strategi untuk memenuhi kebutuhan pangan tersebut yaitu dengan melakukan upaya peningkatan produktivitas dengan cara intensifikasi pertanian salah satunya adalah dengan penerapan teknologi terpadu menggunakan metode System of Rice Intensification (SRI) dan budidaya secara organik. Penelitian telah dilaksanakan pada bulan Maret sampai Juni tahun 2017 di desa Sambirejo, Kecamatan Sukorejo, Kabupaten Sragen. Sebanyak empat puluh varietas padi yang berasal Balai Besar Penelitian Tanaman Padi, Sukamandi, Subang Jawa Barat diuji dalam penelitian ini. Penanaman dilakukan secara pindah tanam dengan menggunakan bibit berumur 15 Hari Setelah Sebar (HSS) sebanyak 1bibit/lubang tanam pada plot berukuran 2 m x 2 m dengan dengan jarak tanam 20 cm x 20 cm. Penelitian ditata dengan menggunakan Rancangan Acak Kelompok Lengkap dengan empat ulangan. Teknik budidaya dilakukan secara organik dengan petunjuk pengelolaan tanaman terpadu. Pengamatan dilakukan terhadap karakter keragaan agronomis dan hasil. Hasil analisis varian menunjukkan terdapat perbedaan antar varietas yang diuji pada semua karakter agronomis dan hasil. Varietas yang memberikan respon yang baik pada lahan organik dengan sistem SRI di lokasi penelitian adalah Varietas Membramo, Sintanur, Inpari 13, Inpari 32 dan Inpari 5 Merawu sedangkan varietas yang memberikan respon kurang baik pada penelitian adalah varietas Batutegi dan Lusi.
Karakterisasi Morfologi 51 Varietas Unggul Baru Padi (Oryza sativa L.) di Kabupaten Subang Simamora, Praneva Annisa; Sugiono, Darso; Widyodaru S, Nurcahyo; Susanto, Untung
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3731

Abstract

Rice (Oryza sativa L.) is a very important commodity. The nutritional content in rice makes rice a very important commodity to meet food needs. One of the efforts that can be made to increase rice productivity is to create new superior rice varieties that have high yields. This study aims to examine and determine the varied morphological characteristics of the new superior varieties of rice, and determine how close the kinship relationship is between 51 new superior varieties of rice (Oryza sativa L.) and determine the advantages of each variety. This research was conducted at the Experimental Garden of the Indonesian Rice Research Center located in Sukamandijaya Village, Ciasem District, Subang Regency, West Java. The time for conducting the research was carried out in September 2021-March 2022. The research method used was a descriptive method, with 51 treatments of new superior rice varieties. Each treatment was repeated 3 times so that there were 153 experimental units. Each observational data on morphological characters of 51 superior rice varieties (Oryza sativa L.) was analyzed by clustering using the UPGMA method with NTSYS 2.02i and 2.11a software. The results showed that the results of the cluster analysis formed 6 main groups at a similarity coefficient value of 0.72 (72%). There are 7 closest kinship relationships and have the farthest kinship in inpari 1 and Inpago 9 varieties. Keywords : Characterization, Morphology, New superior varieties, Clustering, Rice
Hubungan Kekerabatan Beberapa Varietas Unggul Terpilih Tanaman Padi (Oryza sativa L.) Berdasarkan Marka Morfologi Rahma Dini, Nanda Aulia; Azizah, Elia; Samaullah, MY; Susanto, Untung
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3672

Abstract

Differences and similarities in morphological characters in rice plants can be distinguished through characterization activities. Morphological characterization needs to be carried out to identify the superior characters that appear, especially characters related to the growth and development of rice plants which can increase the yield potential of rice. The results of the morphological characterization can be used to determine the close kinship and genetic distance between plants. This study aims to determine the kinship relationship and genetic distance formed in several selected superior varieties of rice plants. The research was conducted at the Experimental Garden of the Sukamandi Rice Research Center. The research method used is descriptive experimental method with a treatment of 44 varieties of rice, repeated 3 times. Observational data were analyzed using Ntsys 2.02 and 2.11 software with the UPGMA method. The results showed that several selected high yielding varieties had similarities in several morphological characters such as leaf width, leaf angle, leaf neck color, leaf midrib color, leaf tongue color and leaf tongue shape. The resulting similarity coefficient is 0.75 and seven groups with close kinship are formed. The genetic distance formed in several selected superior varieties of rice plants has a narrow diversity with a value of 0.25. The results for both parameters indicate that the resulting genetic diversity is small. This cannot be used as a cross parent but can be used as genetic material which is re-analyzed to increase its diversity.
A Study of Genetic Variability 30 Rice Genotypes (Oryza sativa L.) Using Some SSR (Simple Sequence Repeat) Markers Adrift Zn: Studi Variabilitas Genetik 30 Genotipe Padi (Oryza Sativa L.) Menggunakan Beberapa Marka SSR (Simple Sequence Repeat) Terpaut Zn Pangaribuan, Tiwi Rumondang; Syafi’i, Muhammad; Azizah, Elia; Susanto, Untung; Pramudyawardani, Furry; Prastika, Desi
Jurnal Pertanian Tropik Vol. 9 No. 2 (2022): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (732.993 KB) | DOI: 10.32734/jopt.v9i2.8830

Abstract

Rice is one of the important commodities in indonesia. Analysis of rice genetic diversity is necesarry for the succes of the local rice variety program. This study aims to identify the genetic variability and kinship patterns of 30 rice genotypes (Oryza sativa L.) using six zinc-linked SSR (Simple Sequence Repeat) markers. The research was conducted in September - December 2021 at the DNA Laboratory of the Sukamandi Rice Plant Research Center. A total of 30 local rice genotypes with diverse Zinc content have been analyzed using laboratory experiments. The results showed that there were different allele variations (2 – 8) among the genotypes tested with an average number of alleles of 4.5, while the average value of Polymorphism Information Content (PIC) amounted to 0.48 (0.20 - 0.70). 4 SSR markers have PIC values of > 0.5 (RM162, RM38, RM30, and RM80) which show that the markers are informative for the study of rice genetic diversity with Zinc content variety with an average gene diversity value of 0.53. The results of the phylogenetic analysis showed that the 30 genotypes clustered into five clusters with a similar coefficient of 0.68.
Variabilitas Genetik pada Beberapa Varietas Unggul Baru Padi (Oryza sativa L.) Berdasarkan Penanda Morfologi Biji Ardani, Hanna Qotrunnada; Syafii, Muhammad; Azizah, Elia; Susanto, Untung
JURNAL AGROPLASMA Vol 11, No 2 (2024): JURNAL AGROPLASMA VOLUME 11 NO 2 TAHUN 2024
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v11i2.5906

Abstract

Rice (Oryza sativa L.) is known to have a very diverse genetic diversity. The target of rice variety improvement is to produce new varieties that have superior traits in accordance with the development objectives in each typological region. This study aims to determine the level of similarity formed in a number of new superior varieties. The method used is descriptive experimental, with 51 treatments, namely new superior varieties, repeated 3 times so that there are 132 experimental units. Each agronomic character observation data was analyzed by clustering using the UPGMA method. The analysis used NTSYS 2.02i and 2.11a software. The results showed that the genetic variability of 51 new superior varieties of rice (Oryza sativa L.) has a similarity coefficient value of 0.81 based on clustering analysis. This shows a high level of similarity of genetic variability, however, the clustering results form 5 main groups with genetic distances ranging from 0.0000 - 0.5593.  Keywords: genetic variability, seed morphology, new improved varieties, clustering.
DAYA HASIL GALUR-GALUR PADI SAWAH GENERASI LANJUT PADA KONDISI TERCEKAM KEKERINGAN Pramudyawardani, Estria F.; Wening, Rina H.; Susanto, Untung
Agrin Vol 21, No 2 (2017): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2017.21.2.370

Abstract

Kekeringan pada lahan sawah merupakan masalah yang kerap kali menjadi ancaman pada budidaya padi.Penggunaan varietas yang tepat dapat mengurangi resiko kehilangan hasil yang terlalu besar. Penelitian inibertujuan untuk mengetahui keragaan hasil 220 galur padi sawah generasi lanjut di lahan kering dibandingkandengan lahan optimal. Percobaan dilaksanakan pada musim kemarau di kebun percobaan BB Padi Sukamandi,menggunakan rancangan augmented 5 blok dengan 4 varietas pembanding (INPARI 10, INPARI 13, SituBagendit, dan Limboto). Perlakuan lahan optimal sesuai dengan cara budidaya yang disarankan, sedangkan lahankering hanya diairi hingga 4 minggu setelah tanam. Luas plot yang digunakan 1 m x 5 m per galur. Pengamatandilakukan terhadap umur berbunga 10% dan 50% (hss), tinggi tanaman (cm), jumlah anakan dan malai per rumpun,bobot seribu butir (gram), dan hasil per plot (kg) dikonversikan menjadi t/ha GKG. Pengamatan terhadap kondisiair tanah, kelengasan tanah, serta skoring gejala kekeringan ditambahkan untuk lahan kering. Sebanyak 23 galurmemperoleh hasil lebih baik dari Situ Bagendit (2,32 t/ha GKG) di lahan optimal dan 17 galur lebih baik dariLimboto (1,05 t/ha GKG) di lahan kering. Galur BP15704b-14 dan A 62-1 (BP10764f-10-2) teridentifikasi tumbuhbaik di kedua lahan. Tujuh galur dengan produktivitas setara Limboto mempunyai mekanisme ketahanan terhadapkekeringan. Kondisi kekeringan menurunkan tinggi tanaman, bobot seribu butir, dan hasil.Kata kunci: daya hasil, galur padi, lahan kering, kekeringanABSTRACTDrought often pose a threat to rice cultivation because water is an essential requirement in rice growth.Drought tolerant varieties are expected to mitigate the risks of drought stress. This study aims to determine theperformance of 220 advance lines in the dry land as compared with the optimum land. Experiment conducted atSukamandi esperimental station during dry season. Experiment was arranged in augmented 5 blocks with 4 checkvarieties (INPARI 10, INPARI 13, Bagendit, and Limboto). The optimum land treatment irrigated based on therecommended cultivation, while the dry land treatment irrigated until 4 weeks after planting only. The plot areawas 1 m x 5 m per lines. Observations was done for: days to 10% and 50% flowering (DAS), plant height (cm),number of tillers and panicles per hill, thousand grain weight (g), and yield per plot (kg) converted to t/ha. Additionobservations on dry land treatment was done for groundwater, soil moisture, and scoring drought symptom. Theresult showed 23 lines had higher yield than Situ Bagendit (2.32 t/ha) in optimum land, while 17 lines had higheryield than Limboto (1.05 t/ha) in dry land. BP15704b-14 and A 62-1 (BP10764f-10-2) was identified had stableyield on both treatment. Seven lines which similar with Limboto productivity was estimated to have resistancemechanisms for drought stress. Drought stress decline plant height, grain weight, and yield.Key words: potential yield, dry land, drought
Co-Authors Ardani, Hanna Qotrunnada Elia Azizah, Elia Farida Damayanti Lukman, Pradita N. Muhammad Syafii Nono Carsono Pangaribuan, Tiwi Rumondang Pramudyawardani, Estria F. Pramudyawardani, Furry Prastika, Desi Rahma Dini, Nanda Aulia Rina H. Wening, Rina H. Samaullah, MY Sari, Santika Simamora, Praneva Annisa Sugiono, Darso Syafi’i, Muhammad umi barokah Widyodaru S, Nurcahyo