Eni Harmayani
Gadjah Mada University

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

Immobilized Lipase - Catalyzed Fish Oil Hydrolysis in Organic Solvent Tyas Utami; Erryana Martati; Pudji Hastuti; Eni Harmayani
Indonesian Food and Nutrition Progress Vol 6, No 1 (1999)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.70

Abstract

Immobilized lipase from Mucor miehei catalyzed the hydrolysis of cod liver oil in the presence of hexane was studied. The research was conducted to observe the effect of hexane on degree of hydrolysis, glyceride profile and EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) contents. Enzymatic hydrolyses were carried out in a waterbath shaker at 50°C for 48 hours. Reaction mixtures contained of 1 g cod liver oil, hexane, Tris HCl buffer pH 7.5, and 10% (w/w oil) immobilized enzyme. Addition of various amount of hexane decreased the degree of hydrolysis and changed glyceride profile. Hexane to buffer ratio of 1:9 and 9:1 yielded 77% and 64% hydrolyses respectively. The results showed that degree of hydrolysis was affected by the oil concentration in the solvent system, not by the oil concentration in the overall system. It was observed that after hydrolysis, EPA dan DHA contents in the forms of glycreride and free fatty acid were higher in the addition of hexane than that of in the absence of hexane
Supplementation of Indigenous Probiotic Bacteria into Yoghurt Lily Arsanti Lestari; Eni Harmayani; Y. Marsono
Indonesian Food and Nutrition Progress Vol 10, No 1 (2003)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.94

Abstract

Traditional youhurt bacteria, lactobacillus bulgaricus and streptococcus thermohhilus, usually do not survive under acidic conditions and bile concentrations when they encountered in the gastrointestinal tract.Therefore for yughurt to be considered as aprobiotic productct, probiotic bacteria are incorporated as a dietary adjunct.The purpose of this research was to produce acceptable yougurt supplemented with indigenous probiotic bacteria.This research was divied into three parts i.e.addition of indigenous probiotic(Lactobacillus sp.Dad 13 and lactobacillus plantarum Mut7) in standart formula, sensory evalution of yoghurt on appearance, texture, flavor, and everall, and evalution of selected probiotic culture for its viability and capability to grow with yoghurt starter.Lactobascillus sp Dad 13 and lactocillus plantarum Mut 7)in standart formula, sensoriy evalution of youghurt on appearence, texture, flavor, and overall and evalution of selected probiotic culture for its viability and capability to grow with yoghurt starter.Lactobcillus sp dad 13 and lactobacillus plantarum Mut7 were selected as indigenous probiotic culture for its viability and capability to grow with yoghurt starter.Lacrobacillus sp Dad 13 and Lactobacillus plantarum Mut7 were selected as indigeneous probiotics such as acid and bile tolerant, capable of supressing enteric pathogenic bacteria, improving lactic acid bacteria population in colon and also have the ability to lower cholesterol levels.The result s showed that lactobacillus sp.dad 13 produced better flavor compared with yohurt supplemnted with L.plantarum Mut7 and traditional yoghurt without probiotic suplementation. However, suplementation of lactobacillus sp Dad 13 produced lower sensory score on appearance, but similar in texture compared with traditional yoghurt.Supplementation of lactobacillus sp Dad 13 produced sensorically acceptable youghurt and did not significantly different with traditional youghurtThe number of total lactic acid bacteria(LAB) increased during fermentation from 2.45x10 CFU/ml to 2.31 x 10 CFU/ml.Combination of L.bulgaricus and S.thermophilus starter cultures produced highest lactic acid production.However, supplementation of lactobacillus sp Dad 13 did not statically differenton latic acid production,pH and Lab count.Supplementation of probiotic bacteria is important in order to make yoghurt have a better functional food properties.