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All Journal Nexus Kedokteran Komunitas Nexus Biomedika Nexus Kedokteran Translasional
. Suyatmi
Faculty of Medicine, Sebelas Maret University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Pengaruh Pemberian Ekstrak Etanol Bit Merah (Beta vulgaris L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Pangestika, Debora Marga; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Red beet (Beta vulgaris L.) has various components such as betalain, flavonoid, phenolic, β-karoten, vitamin C and E, Fe, Zn, Cu, Mn, and Selenium. These components are potent to be antioxidant to protect liver from free radicals. The aims of this research were to evaluate hepatoprotective effect of ethanol extract of red beets on histologic damage of mice’s liver cells induced by paracetamol and the dose dependent of this extract. Methods: This was laboratory experimental research with the posttest only controlled group design. The subjects of this reaseach were 30 Swiss webster male mice (Mus musculus), 2-3 months old and ± 20 grams of weight, divided into 5 groups randomly. The groups were KK(-), KK(+), KP1, KP2, and KP3. Aquadest was administered to KK(-) and KK(+). Paracetamol was administered to KK(+), KP1, KP2, and KP3 on the day 12, 13 and 14. Red beet extracts were administered to KP1, KP2, and KP3 in dose 5.6 mg,  11.2 mg and 22.4 mg per 20 g BW of mice, successively. The hepatoprotective effects were assessed by counting the number of damage cells among 100 hepatocytes at central lobe zone of liver. Results: The result of One-Way ANOVA p = 0.000 (p < 0.05) showed a significant difference among each other group. The linear regression test p = 0.000 (p < 0.05) showed a correlation between increasing dosage and improving protective effect on mice’s liver cell damage. Conclusions: The ethanol extract of red beet had protective effect on histologic damage of mice’s liver cells induced by paracetamol. The increasing dosage of the red beet extract improved the protective effect to liver cells. Keywords : ethanol extract red beet, mice liver cells, paracetamol 
Efek Nefroprotektif Ekstrak Etanol Buah Jambu Biji Merah (Psidium guajava Linn) terhadap Kerusakan Histologis Ginjal Mencit yang Diinduksi Asetaminofen Priscilla, Prisca; Suyatmi, .; Sari, Yulia
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Red guava fruit (Psidium guajava Linn) contains various of antioxidants such as vitamins A, C, E, minerals Fe, Zn, Se, flavonoids, and lycopene, which they were thought to have nephroprotective effect. The objective of this research was to evaluate the preventive effect of red guava fruit ethanolic extract on tubular renal damage induced by acetaminophen on mice. Methods: Samples were twenty eight male mice, Swiss webster strain, 2-3 months old age and + 20 gram of each weight. Sample divided into 4 groups, each group consists of seven mice. The negative (KK(-)) and positive control group (KK(+)) mice were given aquadest for 14 days. The other group of mice were given red guava fruit ethanolic extract with the dose of 35 mg/20 g body weight of mice (KP1) and the last group of mice were given red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight (KP2) for 14 days. Acetaminophen was given to groups of KK(+), KP1, and KP2. The 15th day, mice were sacrificed and histological preparation were made to evaluate histological damage on rens. Renal histological features were assessed by counting the number of tubular epithelial undergoing pyknosis, karyorrhexis, and karyolysis. Data were analyzed using the One-Way ANOVA test (α = 0.05) and then were continued with Post Hoc Multiple Comparisons (LSD) test (α = 0.05). Results: The mean renal histological damage score was significantly higher on group KK(+)= 40.50+1.991, while the group of KK(-)= 8.93+1.720 as the less. The group of KP2 had fewer numbers of renal histological damage (17.96+1.621) compared to the KP1 group (25.36+1.929). The results of One-Way ANOVA and LSD test showed that there was significant difference between the four groups (p = 0.000). Conclusions: The ethanolic extract of red guava fruit showed nephroprotective effect to the renal histological damage of mice which was induced by acetaminophen. Red guava fruit ethanolic extract with the dose of 70 mg/20 g body weight of mice showed a higher nephroprotective effect than the dose of 35 mg/20 g body weight of mice. Keywords: ethanolic extract, red guava, acetaminophen, renal histological, mice.
Efek Nefroprotektif Ekstrak Daun Sirsak (Annona muricata L.) pada Mencit Model yang Diinduksi Parasetamol Hidayah, Nur; Suyatmi, .; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Soursop Leaf (Annona muricata L.) contains active compounds that have antioxidant effects consisting of flavonoid, alkaloid, tannin, triterpenoid and steroid. The aim of this research is to know the protective effect of Soursop Leaf Extract on the renal histological damage of  mice induced by paracetamol Methods: This study was experimental research with the post test only controlled group design. Samples were 30 male Swiss webster mice, 2-3 months old and ± 20 g of body weight. Samples were divided randomly into 5 groups, each group consisted of 6 mice. The normal group (KN) and negative control group (KKn) were given distilled water for 14 days. KP1, KP2, and KP3 were given soursop leaf extract in dose 5.6 mg, 11.2 mg, and 22.4 mg per g of body weight of mice, respectively, for 14 days. Paracetamol in dose 5.07 g per 20 g body weight of mice were given to KKn, KP1, KP2, and KP3 on the 12th ,13th ,14th days. On the 15th day mice were sacrificed and then renal organ were taken for histological preparation and stained with HE. Renal histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of 50 epithelial cells from proximal tubule of right and left renal organ. Data were analyzed using One-Way ANOVA test and continued with Post Hoc (LSD) test. Results: The means of necrotic cells for KN, KKn, KP1, KP2, and KP3 were 8.17, 38.58, 26.92, 21.08 and 11.67, respectively. The One-Way ANOVA test showed a significant difference of renal damage score in five groups with p = 0.000. The Post Hoc LSD test also showed significant difference among five groups, p = 0.000. Conclusion: Soursop leaf extract can prevent the histological damage of renal cells on mice induced by paracetamol. Keywords: Soursop leaf extract, paracetamol, renal histological damage
Pengaruh Pemberian Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Hepar Mencit (Mus musculus L.) yang Diinduksi Parasetamol Irawan, Alvian Oscar; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Kenikir leaf contains flavonoids, phenolic acid, saponin, carotenoids, vitamin A, vitamin C, Fe, Zn, and Cu. They are potential antioxidants being capable of protecting cells from free radical. The aim of this research is to evaluate the hepatoprotective effect of kenikir (Cosmos caudatus Kunth.) leaf’s ethanol extract on mice induced by paracetamol. Methods: This research was a laboratory-based experiment with the post-test only with control group design. The samples of 35 male Swiss webster mice, aged 2-3 months with ± 20 gram of weight were randomly divided into 5 groups. The groups were normal group (KN), negative control group (KK(-)), treatment group 1 (KP1), treatment group 2 (KP2), and treatment group 3 (KP3). Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg were administered daily for 14 days to KP1, KP2 and KP3, respectively. A toxic dosage of paracetamol was administered to KK(-), KP1, KP2, and KP3 on day 12th, 13th, and 14th. On day 15th, mice were sacrificed by neck dislocation and the liver were prepared and stained with HE for histology study. The degree of damage being due to the paracetamol was based on summation of pyknosis, karyorrhexis, karyolysis nucleus. Data were analyzed using One-Way ANOVA test (α = 0.05), followed by Post Hoc Multiple Comparisons (LSD) (α = 0.05) test. Results: The One-Way ANOVA test result was p = 0.000 (p < α). The statistical LSD test result was p = 0.000 (p < α), which showed significant differences in the hepatic damage between KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3 group. Conclutions: The administration of kenikir leaf’s extract is able to prevent the damage in mice’s liver cells induced by paracetamol. Keyword: ethanol extract, kenikir (Cosmos caudatus Kunth.) leaf, paracetamol, liver cells, mice 
Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Wibowo, Lina Kristanti; Suyatmi, .; Sari, Yulia
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, β-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mices kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (α = 0.05) and Post Hoc Multiple Comparisons test (α = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mices kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mices kidney, Parasetamol 
Efek Hepatoprotektif Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Kumala, Riris Arizka Wahyu; Suyatmi, .; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Soursop leaves (Annona muricata L.) contains alkaloid, flavonoid, saponin, triterpenoid, and steroid that are potent antioxidant to protect the liver from free radicals. The aim of this research was to know the hepatoprotective effect of soursop leaves extract toward liver histological damage of mice (Mus musculus) induced by paracetamol. Methods: This was an experimental laboratory research with the post test only controlled group design. Samples were 30 male Swiss Webster mice with 2-3 months old and ± 20g of weight taken by incidental sampling. They were divided randomly into 5 groups, namely kelompok kontrol negatif (KKn), kelompok kontrol (KK), kelompok perlakuan 1 (KP1), kelompok perlakuan 2 (KP2), and kelompok perlakuan 3 (KP3). KKn and KK were given aquadest for 14 days. Soursop leaves extract were given to KP1, KP2, KP3 in dose 5.6 mg, 11.2 mg, and 22.4 mg/20gbw of mice respectively for 14 days. Paracetamol in dose 5.07 g/20gbw were given to KK, KP1, KP2, and KP3 on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for histological preparation with paraffin block method and HE staining. Liver histological features were assessed based on quantifying of pyknosis, karhyorhexis, and karyolysis of each 100 cells on the 3rd zone. Data were analyzed using the One-Way ANOVA test (α = 0,05) and continued with Post Hoc (LSD) test (α = 0,05). Results: The results of One-Way ANOVA test showed a significant difference of liver damage score among groups, *p = 0.000. Results of LSD test showed significant difference between KKn-KK, KKn-KP1, KKn-KP2, KKn-KP3, KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, KP2-KP3 with *p = 0.000 (p < 0.05). Conclusion: Soursop leaves extract (Annona muricata L.) can prevent liver histological damage of mice (Mus musculus) induced by paracetamol.Keywords: Soursop leaves extract, paracetamol, histological damage of liver cell
Recurrrent Respiratory Infection (RRI) Frequency Influence the Nutritional Status of Children at Jebres Village Surakarta Sienviolincia, Denata; Suhanantyo, .; Suyatmi, .
Nexus Kedokteran Komunitas Vol 6, No 2 (2017): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: One of the things that directly affect the nutritional status is an infection. Acute Respiratory Infections often occur in children aged under 5 years old and often repeated. This research aims to determine the relationship of Reccurrent Respiratory Infection (RRI) frequency and the nutritional status of children under five years old. Methods : This research was analytical observational with case control design. This research was conducted at several posyandu of Jebres Village, Jebres Subdistrict, Surakarta, on November 2015. Sampling was done by purposive sampling technique. Sixty seven samples was classified into frequently RRI (30 children) designed as case group and infrequently RRI (37 children) as control group. The nutritional status was determined by W/A and H/A index interpreted into z-score. Data of RRI frequency obtained from questionnaires filled out by the mother. The data obtained were processed using SPSS for Windows 18.0 and analyzed by independent t test to analyze the difference of nutrional status at significance level of ? = 0.05. Results : Children who rarely affected by RRI has average z-score -0.61 SD for W/A and -0.86 SD for H/A. While children who often exposed RRI has average z-score -1.12 SD for W/A and -1.49 SD for H/A. The result of this research showed that there was significant difference of nutrional status between case and control group. RRI frequency with W/A index (p = 0,017) and RRI frequency with H/A index (p = 0,008). Conclusion : the significant difference of nutritional status between groups indicating  a relationship between Reccurrent Respiratory Infection (RRI) frequency and the nutritional status of children at Jebres Village Surakarta. Key words : Reccurrent Respiratory Infection (RRI), nutritional status
Co-Authors . Suhanantyo A.A. Ketut Agung Cahyawan W Alvian Oscar Irawan, Alvian Oscar Debora Marga Pangestika Lina Kristanti Wibowo, Lina Kristanti Prisca Priscilla Ratih Puspita Febrinasari Riris Arizka Wahyu Kumala, Riris Arizka Wahyu Sienviolincia, Denata Tamaulina Br Sembiring