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All Journal Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology
Khairunnisa Sy
Cancer Chemoprevention Research Center Faculty of Pharmacy, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Computer Science & IT Medicine & Pharmacology

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Elettaria cardamomum Distillate Increases Cellular Immunity in Doxorubicin Treated Rats Raksamiharja, Rikat; Sy, Khairunnisa; S, Meirizky Zulharini; Novarina, Annisa; Sasmito, Ediati
Indonesian Journal of Cancer Chemoprevention Vol 3, No 3 (2012)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Doxorubicin is one of chemotherapeutic agent used for cancer therapy. However, doxorubicin usage causes some adverse effects, such as lymphocyte, CD4+ and CD8+ cells number.  Therefore,  the  co-chemotherapeutic  agent  is  required  to  reduce  the imunosuppression  effect.  Cardamom  (Elettaria  cardamomum)  contains  terpenoid  1,8  cineol. This  research  aimed  to  know  the  effect  of  Elettaria  cardamomum  distillate  (ECD)  in combination with doxorubicin on Sprague Dawley rat’s hematology profile and the amount of CD4+  and  CD8+  cells.  The  experiment  was  done  for  13  days  using  6  groups  of  rats:  I doxorubicin (dox) 15 mg/kg BW; II dox + ECD5 mg/kg BW; III dox + ECD 50 mg/kg BW; IV dox  +  ECD  100  mg/kg  BW;  V  ECD  100  mg/kg  BW;  VI  control  without  treatment.  The hematology  profile  and  the  amount  of  CD4+  and  CD8+  were  counted  before  and  after treatment  using  flowcytometer.  The  results  show  that  ECD  increases  the  amount  of lymphocyte, white blood, CD4+ and CD8+ cells in dose dependent manner in doxorubicin treated rats. Based on the data, it can be concluded that ECD is potential to be developed as immunostimulant agent for chemotherapy.Keywords: Cardamom (Elettaria cardamomum), immunostimulant, hematology, CD4+, CD8+
The transformation of dbr2 and p19 Genes into Artemisia annua L. by Agrobacterium tumefaciens Mediation: Metabolites Analysis Sy, Khairunnisa; Kristianti, Tati; Suhandono, Sony; Elfahmi, Elfahmi
Indonesian Journal of Pharmaceutical Science and Technology 2024: Suppl. 6, no. 3 (The 3rd Mandala Waluya International Conference on Pharmaceutical Science and
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v6i3.54535

Abstract

The low level of Artemisinin in Artemisia annua L. is associated with its biosynthetic pathway. Genetic engineering offers a potential solution to increase its metabolites. The research objectives were to transform the double bond reductase (dbr2), one of the critical genes in the biosynthesis of Artemisinin, and p19, an anti-silencing gene, into A. annua L. by the mediation of Agrobacterium tumefaciens and assess their impact on metabolites production. The dbr2 and p19 genes were verified by analyzing PCR products. Plasmids pCAMBIA1303-DBR2-P19 were transformed into A. tumefaciens and subsequently introduced into the leaves from tissue culture of A. annua L, using vacuum infiltration and assessed by GUS assay. UPLC-ESI-MS/MS measured artemisinic acid (AA), dihydroartemisinic acid (DHAA), and Artemisinin contents. The transformation in A. tumefaciens was successful using a freeze-thaw method. The tissue culture of A. annua L. has been infected by A. tumefaciens using vacuum infiltration. Based on the GUS histochemical assay, the gene has been successfully inserted in the leaves with an efficiency of 48.2%. The result from UPLC-ESI-MS/MS showed that the level of Artemisinin in the transformation sample with and without pCAMBIA-dbr2-p19 was detected but not quantifiable while in wild-type leaves were quantified at 0.008% in fresh weight. The AA and DHAA were not detectable but only in the wild type. The transformation was successful, but the quantification of DHAA and AA was unsuccessful because of the low quantity in the samples.
Co-Authors Annisa Novarina Ediati Sasmito Elfahmi Elfahmi, Elfahmi Meirizky Zulharini S Rikat Raksamiharja SONY SUHANDONO TATI KRISTIANTI