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SERAPAN SIANIDA (CN) PADA Mikania cordata (Burm.f) B.L. Robinson, Centrosema pubescens Bth DAN Leersia hexandra Swartz YANG DITANAM PADA MEDIA LIMBAH TAILING TERKONTAMINASI CN Syarif, Fauzia
Jurnal Teknologi Lingkungan Vol. 10 No. 1 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Some plant species growing in the contaminated areas, indicated high toleranceand potentially affective in accumulating pollutants in their roots and above groundportions. These plants can be utilized as hyperaccumulators for cleaning up thecontaminated sites. Study on heavy metal and CN contamination and potentialplant species for accumulator is urgently needed in order to understand the problemsand to obtain suitable technology for the solution. This research aims to examineCN accumulator plants growing in CN contaminated tailing to find a possible solutionof cleaning up by using green technology of phytoremediation. Phytoremediation isdefined as clean up of pollutants primarily mediated by photosynthetic plants. Thisstudy aims to characterized plants that grow under extreme contaminated media ofgold mined tailing and to analyse their potencies as hyperaccumulators. Mikaniacordata (Burm.f) B.L.Robinson,Centrosema pubescens Bth and Leersia hexandraSwartz which proven tolerant and dominant in the contaminated site were examinedin this research. The plants were grown in tailing waste media added by 0 ppm CN,2.5 ppm CN, 5 ppm CN dan 7.5 ppm CN using complete randomized design with 5replicates. The results showed that the plants were capable of growing under thehighest level of CN. Among three species, Mikania cordata showed the highestbiomass production followed by Centrosema pubescens and Leersia hexandra. TotalCN accumulation varied between species, the highest was reached in 2.5 ppm CNtreatment i.e. 22.48 mg/kg in Leersia hexandra, followed by Centrosema pubescens(18.92 mg/kg) and Mikania cordata (12.03 mg/kg). The highest CN content was0.085 mg in Mikania cordata treated with 7.5 ppm CN. High ratio of shoot to root CN(>1) was expected in hyperaccumulator plants to indicate that CN was more distributedin the above ground portions than in the roots. In this study the highest shoo to rootCN ratio was showed in Mikania cordata i.e.11.75

RESPON PUPUK DAUN NPK TERHADAP PERTUMBUHAN Nepenthes ventrata DAN Nepenthes neglecta Mansur, Muhammad; ., Saefudin; Syarif, Fauzia
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Leaf NPK fertilizer experiments on pitcher plant (Nepenthes neglecta and ventrata) conductedÃ‚Â in May 2008 to March 2009 in a greenhouse, Botany Division, Research Center for Biology-LIPI, Cibinong Science Center, Cibinong. The objective is to study the effect of leaves NPKÃ‚Â fertilizer on the growth of the two Nepenthes species tested. Experiments using Random Complete Factorial Design with two factors, namely the two Nepenthes species and threedoses of fertilizer each treatment repeated six times. Growth parameters measured include;Ã‚Â long shoot leaves, plant height, number of leaves, number of mature pitchers and growthÃ‚Â pitchers. Results showed that the dose of NPK fertilizer provided (2 and 4 ml / lt) have no effectÃ‚Â on plant height and number of leaves, but little effect on the number of mature pitchers thanthe control plants. The statistical tests also showed no interaction between the two NepenthesÃ‚Â species with a given dose of fertilizer on the growth of plants. Minimum-maximum temperatureÃ‚Â recorded in the greenhouse during the experiment was 22.20C and 38.50C. Meanwhile, airÃ‚Â humidity between 43,6-64,7%.Key words: Leaf NPK fertilizer, plant growth, pitcher plant

POTENSI SALVINIA MOLESTA D.S. MITCHELL, LIMNOCHARIS FLAVA (L.) BUCHENAU DAN MONOCHORIA VAGINALIS (BURM.F.) PRESL UNTUK FITOEKSTRAKSI MERKURI DI SAWAH YANG TERCEMAR MERKURI AKIBAT KEGIATAN PENAMBANG EMAS TANPA IZIN (PETI) Juhaeti, Titi; Hidayati, Nuril; Syarif, Fauzia; Hidayat, Syamsul
Jurnal Teknologi Lingkungan Vol. 11 No. 2 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

The research were carried out to study the potency of Salvinia molesta D.S. MitchellÃ‚Â (Kiambang), Limnocharis flava (L.) Buchenau (Genjer) and Monochoria vaginalisÃ‚Â (Burm.f.) Presl (Eceng) for Hg phytoextraction on paddy field contaminated with HgÃ‚Â from illegal mining. The plants were grown on contaminated media from paddy field inÃ‚Â Pongkor (68.269 ppm Hg) added by NPK fertilizer (0, 3 and 6 g/pot) and ammoniumÃ‚Â thiosulphate chelating agent (0, 20 ppm). The result showed that the growth of kiambang,eceng, genjer and padi are significantly different. Chelating agent did not significantlyÃ‚Â affect the plant growth, meanwhile fertilizer significantly affected the plant growth. Thetreatments resulted in different Hg concentration in the plants. Fertilizer increased plantÃ‚Â biomass and so plant Hg content since Hg contents is a function of total biomass andÃ‚Â Hg concentration. It can be concluded that based on the criteria of accumulator plant,kiambang is the most potentially Hg accumulator followed by genjer and eceng.Key words: Phytoextraction, Hg, Salvinia molesta, Limnocharis flava, Monochoriavaginalis

PEMANFAATAN SALVINIA MOLESTA D.S. MITCHELL, AKUMULATOR MERKURI DI SAWAH TERCEMAR LIMBAH PENAMBANGAN EMAS Hidayati, Nuril; Syarif, Fauzia; Juhaeti, Titi
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Mercury is one of important contaminants in mine lands. One approach to remediaterisks from this metal pollutant is phytoextraction using hyperaccumulator plants. Theseremarkable plant species accumulate appreciable high concentrations of metals thando normal plants when the normal plants suffer yield reduction from metal phytotoxicity.Some plant species growing in gold mine contaminated areas indicated high toleranceand potentially effective in accumulating mercury in their roots and above groundportions. Salvinia molesta is one of them. This plant could be utilized as hyperaccumulatorfor cleaning up mercury contaminated sites. This research aim to study phytoextractionof mercury by Salvinia molesta and the effectiveness of mercury degradator bacteria onplant tolerance and mercury phytoextraction. In this study Salvinia molesta was grown inmercury contaminated liquid gold mine waste, added with Mercury (II) Chloride (HgCl2)0 ppm Hg, 10 ppm Hg, 30 ppm Hg and 50 ppm Hg. Mercury degradator bacteria wasapplied in the media. The results showed that Salvinia molesta was able to survive evenin media with high level of mercury concentration (50 ppm), although the number ofplant survival tend to decrease with the increase of mercury concentration. Variables ofsurface coverage and live plants decreased with the increase of mercury concentrationin the media. There was a close correlation between plant growth variables and mercuryconcentration in the media. The effects of mercury toxicity on plants seems to decreasein bacteria treated plants. It was indicated by the higher percentage of surface coverageand plant survival in bacteria treated plants than that of untreated plants up to 30 ppm Hg.Recovery from toxicity was shown in bacteria treated plants up to 30 ppm Hg. Mercuryaccumulation in plants tent to increase with the increase of mercury concentration inmedia. It can be concluded that plant performance was better and mercury concentrationdecreased in bacteria treated plants.

Fitoekstraksi Sianida pada Centrosema pubescens Benth yang Tumbuh di Limbah Tailing Terkontaminasi Sianida Syarif, Fauzia; Hidayati, Nuril; Juhaeti, Titi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Contamination of mined soil and water affects not only to agriculture system but alsofood chains and epidemiological problems. As soil metal can not be biodegraded,remediation of soil heavy metal risks has been a difficult and expensive goal. Presentlythere are several different strategies available for the clean up and restoration ofcontaminated soils. One approach to minimize risks from some toxic pollutants isphytoextraction using hyperaccumulator plants, known as phytoremediation. In thisresearch Centrosema pubescens was studied to examine its potency ashyperaccumulator against cyanide. Although cyanide is not categorized as heavymetal, its presence is considered as one of important toxic pollutants in theenvironments. Detoxification of cyanide (CN) contaminated soils and waters withplants seems to be a feasible option. Centrosema pubescens which proven tolerant anddominant in the CN contaminated environment was used in this research. The plantswere grown in tailing waste media added by 0, 10, 20 and 30 ppm CN. Different pHlevels were applied, i.e.+ 6 and +5. The results showed that the plants were capable ofgrowing under high level of CN. The results indicated that Centrosema pubescens canbe considered as high tolerance and potentially effective in accumulating CN, i.e up to3.564 ppm in roots and up to 3.564 ppm in shoot with concentration ratio (shoot/root)up to 1.13. The plants also indicated high tolerance by producing high biomass underhigh level of CN, i.e up to 36.517 g (under 20 ppm CN).

PERTUMBUHAN DAN PRODUKSI TANAMAN JEWAWUT [SETARIA ITALICA (L.) P. BEAUV.] PADA PERLAKUAN DOSIS RADIASI DENGAN PENGURANGAN FREKUENSI PENYIRAMAN UNTUK SELEKSI TOLERAN KEKERINGAN Hidayati, Nuril; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3811

ABSTRACTFoxtail millet [Setaria italica (L.) Beauv.] is one of potential cereal plants that can be developed as a source of carbohydrates in marginal and dry lands. The purpose of this study was to examine the tolerance to drought of foxtail millet accession of Buru Merah as a result of gamma radiation treatment. In this study, the seeds of the Buru Merah accession were treated with gamma ray at doses of 0, 25, 50, 100 and 200 Gy. The established growing plants were then treated with different intervals of watering, which were every day (as control), 2 days, 4 days, and 6 days which were arranged in factorial randomized block design with three replications. The observed variables were increase plant height, increase of leaf number, leaf chlorophyll content, plant water potential and yields. The results revealed that the radiation treatment did not show any significant difference in the production of plant biomass. With radiation treatments of 0, 25, 50 and 100 Gy. and a two-day watering interval the plants can still maintain its water potential above -3 MPa and showing optimum growth. However radiation treatment resulted a significant difference in panicle production, especially between the radiation dose of 200 Gy. and others. The combination of 0-100 Gy. radiation treatment and 2-day watering interval produced plants with optimum panicle production.Keywords: drought, growth, millet, production, radiation, tolerant

POTENSI HIPERTOLERANSI CALOPOGONIUM MUCUNOIDES, CENTROSEMA PUBESCENS DAN CAJANUS CAJAN YANG TUMBUH PADA LIMBAH PENAMBANGAN EMAS TERKONTAMINASI SIANIDA DAN MERKURI Syarif, Fauzia; Hidayati, Nuril; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 4, No 4 (2007): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i4.3251

ABSTRACTPotency of Hypertolerance of Calopogonium mucunoides, Centrosema pubescensand Cajanus cajan . Grown on Gold Mine waste Media Contaminated by Cyanideand Mercury. Cleaning up contaminated environment by using green technology ofphytoremediation is urgently needed in the future. These plants will be utilized ashyperaccumulators for cleaning up the contaminated sites. The results of plant screeningshowed that some plant species, which grow in the contaminated areas, indicated hightolerance and potentially effective in accumulating cyanide and mercury in their rootsand above ground portions. Those plants are Calopogonium mucunoides and Centrosemapubescens. This research aims to examine the potency of three candidate plants i.eCalopogonium mucunoides, Centrosemapubescens and Cajanus cajan, that were grownunder three different media i.e tailing waste of PT ANTAM contaminated by cyanide,public mine or PET1 waste that were contaminated by mercury and non contaminated topsoil as a control. The results showed that all of the species were able to grow undercontaminated media and showed a considerable tolerance by indicated high cyanide andmercury accumulation in their shoot and root. Among those three plants, Centrosemapubescens showed the highest level of cyanide and mercury accumulation i.e 3.29 mg I-' CN (in the shoot), 34.72 mg I-' Hg (in the shoot) and 17.47 mg lk' Hg (in the root),followed by Calopogonium mucunoides i.e 14,97 mg I-' CN (in the root). Concentrationratio of CN and Hg accumulation in shoot/root of Centrosemapubescens was >I indicatingthat this plant, according to the definition can beconsidered as a hyperaccumulator.Key Words: Calopogonium mucunoides, Centrosemapubescens, Cajanus cajan, mercury,cyanide, waste

EFEKTIVITAS EDTA (ETHYLENEDIAMINETETRACETIC ACID) DALAM MENINGKATKAN AKUMULASI TIMBAL PADA SACCHARUM SPONTANEUM YANG 'IHMBUH DI LIMBAH PENAMBANGAN EMAS Hidayati, Nuril; Juhaeti, Titi; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i1.3274

ABSTRACTEffectivity of EDTA (EthylenediaminetetraceticAcid) in Increasing Lead Accumulationof Saccharum spontaneum Grown Under Gold Mine Waste. Lead (Pb) isknown as one of a major metal contaminant in mine tailing. This metal has low solubility,and in many cases, is not readily available. In most soils capable of supporting plantgrow, the soluble Pb2+ levels are relatively low and will not promote substantial uptake bythe plant. In addition, many plants retain Pb2+ in their roots with only minimal trabsportto the aboveground plant portions. Therefore, it is important to find ways to enhance thebioavailability of Pb2+ or to find specific plants that can better translocate the Pb2+ intoharvestable portions for phytoremediation. The success of Pb phytoremediation is tohave significant Pb availability as well as a large quantity of plant biomass with high rateof growth. This experiment was conducted to obtain both purposes. Saccharumspontaneum which proven tolerant and dominant in contaminated area as well as potentialin producing high biomass was used in this research.The plants were grown in wastemedia added by 100 ppm, 200 ppm and 300 ppm of Pb. Ethylenediaminetetracetine Acid(EDTA) was applied to increase Pb availability and plant uptake as well as translocationto the aboveground portions. The results showed that the plant still capable of growingunder the highest level of Pb. EDTA increase Pb availability and plant uptake. Pb accumulationin the aboveground biomass of EDTA treated plants were relatively higher thanuntreated plants.Key words: Timbal, EDTA, phytoremediation

RESPON FISIOLOGIS DAN PERTUMBUHAN KAKAO (THEOBROMA CACAO), KOPI (COFFEA ARABICA), KARET (HEVEA BRASILIENSIS) DAN CENGKIH (SYZYGIUM AROMATICUM ) FASE BIBIT TERHADAP NAUNGAN DAN PEMUPUKAN Hidayati, Nuril; Juhaeti, Titi; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2154

Plants show different physiological responses to light intensities. Study on optimum light intensities for different plants is important, especially for commercial plant commodities, such as industrial plantation plants. This research aims to study the influence of shading and fertilization treatments on plant growth and the activity of some physiological process of four industrial trees namely Cacao (Theobroma cacao), Coffee (Coffea arabica ),Â Rubber (Hevea brasiliensis) danÂ Clove (Syzygium aromaticum). This research was conducted at Cibinong Science Center LIPI. Two treatment factors applied were three levels of shading : 0%, 55%, 75% and three levels of N ferlitizer: 0 g/plant, 5 g/plant and 10 g/plant arranged in Randomized Block Design with five replicates. Result of experiment revealed that all plant species showed the best growth performance and optimum physiological activities under 55% of shade level in combination with 10 g of N fertilization. The highest CO2 was 13.07 Âµmolm-2s-1 Â (55%Â shad). The highest values of transpiration and stomatal conductance were 7.56 molm-2s-1 dan 0.73 molm-2s-1 (55% shade). The highest carbohydrate content was 22.49% (under 0% shade) and the lowest was 12.74% (under 75% shade).Â Keywords: Physiological activity, growth, shading, fertilizationÂ

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